Role of mir-33a, mir-203b, mir361-3p, and mir-424 in hepatocellular carcinoma
This work is licensed under a Creative Commons Attribution 4.0 International License..
Background/aim: Hepatocellular carcinoma (HCC) is one of the most aggressive cancer types. MicroRNAs (miRNAs) are small noncoding regulatory RNAs that function posttranscriptionally. miRNA deregulation was observed in the development and progression of HCC. In this study, we aimed to investigate the expression levels of four miRNAs (mir-33a, mir-203b, mir361-3p, and mir-424) in HCC patients in comparison to healthy individuals.
Materials and methods: Venous blood samples were collected from both HCC patients and healthy individuals. In order to determine the relative expression levels of mir-33a, mir-203b, mir361-3p, and hsa-mir-424 in HCC patients, probe-based quantitative real time PCR (qRT-PCR) was performed. The cycle threshold (Ct) results were analyzed according to the 2−∆∆Ct method and statistical analyses were performed by SPSS Statistics version 15 for Windows.
Results: qRT-PCR analysis revealed that the expression levels of mir-33a (fold change: 7.3 and P < 0.001), mir-203b (fold change: 4.6 and P < 0.001), and mir361-3p (fold change: 5.1 and P < 0.001)were downregulated compared to healthy individuals and mir-424 did not show any significant change between HCC patients and controls.
Conclusion: Our results indicated that mir-33a, mir-203b, and mir-361-3p may significantly contribute to tumor pathogenesis in HCC and have potential to be used as a noninvasive biomarker for cancer therapy.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:51 |
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| Enthalten in: |
Turkish journal of medical sciences - 51(2021), 2 vom: 30. Apr., Seite 638-643 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Yalçınkaya, Burhanettin [VerfasserIn] |
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| Links: |
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| Themen: |
HCC |
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| Anmerkungen: |
Date Completed 10.12.2021 Date Revised 14.12.2021 published: Electronic Citation Status MEDLINE |
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| doi: |
10.3906/sag-2004-214 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM316671207 |
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| 500 | |a Date Completed 10.12.2021 | ||
| 500 | |a Date Revised 14.12.2021 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a This work is licensed under a Creative Commons Attribution 4.0 International License. | ||
| 520 | |a Background/aim: Hepatocellular carcinoma (HCC) is one of the most aggressive cancer types. MicroRNAs (miRNAs) are small noncoding regulatory RNAs that function posttranscriptionally. miRNA deregulation was observed in the development and progression of HCC. In this study, we aimed to investigate the expression levels of four miRNAs (mir-33a, mir-203b, mir361-3p, and mir-424) in HCC patients in comparison to healthy individuals | ||
| 520 | |a Materials and methods: Venous blood samples were collected from both HCC patients and healthy individuals. In order to determine the relative expression levels of mir-33a, mir-203b, mir361-3p, and hsa-mir-424 in HCC patients, probe-based quantitative real time PCR (qRT-PCR) was performed. The cycle threshold (Ct) results were analyzed according to the 2−∆∆Ct method and statistical analyses were performed by SPSS Statistics version 15 for Windows | ||
| 520 | |a Results: qRT-PCR analysis revealed that the expression levels of mir-33a (fold change: 7.3 and P < 0.001), mir-203b (fold change: 4.6 and P < 0.001), and mir361-3p (fold change: 5.1 and P < 0.001)were downregulated compared to healthy individuals and mir-424 did not show any significant change between HCC patients and controls | ||
| 520 | |a Conclusion: Our results indicated that mir-33a, mir-203b, and mir-361-3p may significantly contribute to tumor pathogenesis in HCC and have potential to be used as a noninvasive biomarker for cancer therapy | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a HCC | |
| 650 | 4 | |a Mir-33a | |
| 650 | 4 | |a mir-203b | |
| 650 | 4 | |a mir-424 | |
| 650 | 4 | |a mir361-3p | |
| 650 | 4 | |a qRT-PCR | |
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| 650 | 7 | |a MIRN361 microRNA, human |2 NLM | |
| 650 | 7 | |a MIRN424 microrna, human |2 NLM | |
| 650 | 7 | |a MicroRNAs |2 NLM | |
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| 700 | 1 | |a Taştekin, Didem |e verfasserin |4 aut | |
| 700 | 1 | |a Pençe, Sadrettin |e verfasserin |4 aut | |
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