Imbalance of Regulatory and Cytotoxic SARS-CoV-2-Reactive CD4+ T Cells in COVID-19
Crown Copyright © 2020. Published by Elsevier Inc. All rights reserved..
The contribution of CD4+ T cells to protective or pathogenic immune responses to SARS-CoV-2 infection remains unknown. Here, we present single-cell transcriptomic analysis of >100,000 viral antigen-reactive CD4+ T cells from 40 COVID-19 patients. In hospitalized patients compared to non-hospitalized patients, we found increased proportions of cytotoxic follicular helper cells and cytotoxic T helper (TH) cells (CD4-CTLs) responding to SARS-CoV-2 and reduced proportion of SARS-CoV-2-reactive regulatory T cells (TREG). Importantly, in hospitalized COVID-19 patients, a strong cytotoxic TFH response was observed early in the illness, which correlated negatively with antibody levels to SARS-CoV-2 spike protein. Polyfunctional TH1 and TH17 cell subsets were underrepresented in the repertoire of SARS-CoV-2-reactive CD4+ T cells compared to influenza-reactive CD4+ T cells. Together, our analyses provide insights into the gene expression patterns of SARS-CoV-2-reactive CD4+ T cells in distinct disease severities.
| Errataetall: | |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:183 |
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| Enthalten in: |
Cell - 183(2020), 5 vom: 25. Nov., Seite 1340-1353.e16 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Meckiff, Benjamin J [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 11.12.2020 Date Revised 10.02.2024 published: Print-Electronic UpdateOf: bioRxiv. 2020 Jun 13;:. - PMID 32587963 Citation Status MEDLINE |
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| doi: |
10.1016/j.cell.2020.10.001 |
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| PPN (Katalog-ID): |
NLM316648876 |
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| 245 | 1 | 0 | |a Imbalance of Regulatory and Cytotoxic SARS-CoV-2-Reactive CD4+ T Cells in COVID-19 |
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| 500 | |a Date Revised 10.02.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a UpdateOf: bioRxiv. 2020 Jun 13;:. - PMID 32587963 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Crown Copyright © 2020. Published by Elsevier Inc. All rights reserved. | ||
| 520 | |a The contribution of CD4+ T cells to protective or pathogenic immune responses to SARS-CoV-2 infection remains unknown. Here, we present single-cell transcriptomic analysis of >100,000 viral antigen-reactive CD4+ T cells from 40 COVID-19 patients. In hospitalized patients compared to non-hospitalized patients, we found increased proportions of cytotoxic follicular helper cells and cytotoxic T helper (TH) cells (CD4-CTLs) responding to SARS-CoV-2 and reduced proportion of SARS-CoV-2-reactive regulatory T cells (TREG). Importantly, in hospitalized COVID-19 patients, a strong cytotoxic TFH response was observed early in the illness, which correlated negatively with antibody levels to SARS-CoV-2 spike protein. Polyfunctional TH1 and TH17 cell subsets were underrepresented in the repertoire of SARS-CoV-2-reactive CD4+ T cells compared to influenza-reactive CD4+ T cells. Together, our analyses provide insights into the gene expression patterns of SARS-CoV-2-reactive CD4+ T cells in distinct disease severities | ||
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| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
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| 700 | 1 | |a Chee, Serena J |e verfasserin |4 aut | |
| 700 | 1 | |a Kusnadi, Anthony |e verfasserin |4 aut | |
| 700 | 1 | |a Simon, Hayley |e verfasserin |4 aut | |
| 700 | 1 | |a Eschweiler, Simon |e verfasserin |4 aut | |
| 700 | 1 | |a Grifoni, Alba |e verfasserin |4 aut | |
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| 700 | 1 | |a Weiskopf, Daniela |e verfasserin |4 aut | |
| 700 | 1 | |a Sette, Alessandro |e verfasserin |4 aut | |
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| 700 | 1 | |a Seumois, Grégory |e verfasserin |4 aut | |
| 700 | 1 | |a Ottensmeier, Christian H |e verfasserin |4 aut | |
| 700 | 1 | |a Vijayanand, Pandurangan |e verfasserin |4 aut | |
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