Kinetic studies of galectin-10 release from eosinophils exposed to proliferating T cells
© 2020 British Society for Immunology..
Galectin-10 is involved in the T cell suppressive activity of regulatory T cells and eosinophils alike. We have identified a subpopulation of T cell suppressive eosinophils that express CD16 on the surface and contain more galectin-10 compared with conventional CD16-negative eosinophils. Our main goal was to determine how the intracellular protein galectin-10 is released from eosinophils when exposed to proliferating T cells and if such release could be inhibited. Confocal microscopy and imaging flow cytometry were used to study the release of galectin-10 from eosinophils incubated with polyclonally activated T cells. T cell proliferation was monitored by measurement of the incorporation of [3 H]-thymidine. Initially, galectin-10-containing synapses formed between eosinophils and T cells. Subsequently, the plasma membrane of eosinophils began to disintegrate and cap-like accumulations of galectin-10 budded on the eosinophil cell surface. Lastly, eosinophil extracellular traps composed of nuclear DNA and galectin-10 were freed. It was solely the CD16-expressing suppressive eosinophils that formed synapses and eosinophil extracellular traps containing galectin-10. Dissolution of the extracellular traps by DNase I partly abrogated the T cell suppression exerted by eosinophils. Extracellular trap formation has mainly been associated with anti-bacterial defense, but we show a new putative function of these cellular formations, as mediators of T cell suppression by enabling the release of galectin-10 from eosinophils.
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2021 |
---|---|
Erschienen: |
2021 |
Enthalten in: |
Zur Gesamtaufnahme - volume:203 |
---|---|
Enthalten in: |
Clinical and experimental immunology - 203(2021), 2 vom: 28. Feb., Seite 230-243 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Lingblom, C [VerfasserIn] |
---|
Links: |
---|
Themen: |
Eosinophils |
---|
Anmerkungen: |
Date Completed 16.08.2021 Date Revised 02.02.2022 published: Print-Electronic Citation Status MEDLINE |
---|
doi: |
10.1111/cei.13540 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
NLM316493295 |
---|
LEADER | 01000naa a22002652 4500 | ||
---|---|---|---|
001 | NLM316493295 | ||
003 | DE-627 | ||
005 | 20231225161212.0 | ||
007 | cr uuu---uuuuu | ||
008 | 231225s2021 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.1111/cei.13540 |2 doi | |
028 | 5 | 2 | |a pubmed24n1054.xml |
035 | |a (DE-627)NLM316493295 | ||
035 | |a (NLM)33080067 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Lingblom, C |e verfasserin |4 aut | |
245 | 1 | 0 | |a Kinetic studies of galectin-10 release from eosinophils exposed to proliferating T cells |
264 | 1 | |c 2021 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Completed 16.08.2021 | ||
500 | |a Date Revised 02.02.2022 | ||
500 | |a published: Print-Electronic | ||
500 | |a Citation Status MEDLINE | ||
520 | |a © 2020 British Society for Immunology. | ||
520 | |a Galectin-10 is involved in the T cell suppressive activity of regulatory T cells and eosinophils alike. We have identified a subpopulation of T cell suppressive eosinophils that express CD16 on the surface and contain more galectin-10 compared with conventional CD16-negative eosinophils. Our main goal was to determine how the intracellular protein galectin-10 is released from eosinophils when exposed to proliferating T cells and if such release could be inhibited. Confocal microscopy and imaging flow cytometry were used to study the release of galectin-10 from eosinophils incubated with polyclonally activated T cells. T cell proliferation was monitored by measurement of the incorporation of [3 H]-thymidine. Initially, galectin-10-containing synapses formed between eosinophils and T cells. Subsequently, the plasma membrane of eosinophils began to disintegrate and cap-like accumulations of galectin-10 budded on the eosinophil cell surface. Lastly, eosinophil extracellular traps composed of nuclear DNA and galectin-10 were freed. It was solely the CD16-expressing suppressive eosinophils that formed synapses and eosinophil extracellular traps containing galectin-10. Dissolution of the extracellular traps by DNase I partly abrogated the T cell suppression exerted by eosinophils. Extracellular trap formation has mainly been associated with anti-bacterial defense, but we show a new putative function of these cellular formations, as mediators of T cell suppression by enabling the release of galectin-10 from eosinophils | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Research Support, Non-U.S. Gov't | |
650 | 4 | |a eosinophils | |
650 | 4 | |a extracellular traps | |
650 | 4 | |a galectin-10 | |
650 | 4 | |a human | |
650 | 4 | |a immune synapses | |
650 | 7 | |a Galectins |2 NLM | |
650 | 7 | |a Receptors, IgG |2 NLM | |
700 | 1 | |a Andersson, K |e verfasserin |4 aut | |
700 | 1 | |a Wennerås, C |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Clinical and experimental immunology |d 1966 |g 203(2021), 2 vom: 28. Feb., Seite 230-243 |w (DE-627)NLM00001527X |x 1365-2249 |7 nnns |
773 | 1 | 8 | |g volume:203 |g year:2021 |g number:2 |g day:28 |g month:02 |g pages:230-243 |
856 | 4 | 0 | |u http://dx.doi.org/10.1111/cei.13540 |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 203 |j 2021 |e 2 |b 28 |c 02 |h 230-243 |