A Comparison of Cell Activation, Exhaustion, and Expression of HIV Coreceptors and Restriction Factors in HIV-1- and HIV-2-Infected Nonprogressors
Human immunodeficiency viruses induce rare attenuated diseases due either to HIV-1 in the exceptional long-term nonprogressors (LTNPs) or to HIV-2 in West Africa. To better understand characteristics of these two disease types we performed a multiplex comparative analysis of cell activation, exhaustion, and expression of coreceptors and restriction factors in CD4 T cells susceptible to harbor those viruses. We analyzed by flow cytometry the expression of HLA-DR, PD1, CCR5, CXCR6, SAMHD1, Blimp-1, and TRIM5α on CD4 T cell subsets from 10 HIV-1+ LTNPs and 14 HIV-2+ (12 nonprogressors and 2 progressors) of the ANRS CO-15 and CO-5 cohorts, respectively, and 12 HIV- healthy donors (HD). The V3 loop of the HIV-1 envelope from 6 HIV-1+ LTNPs was sequenced to determine the CXCR6-binding capacity. Proportions of HLA-DR+ and PD1+ cells were higher in memory CD4 T subsets from HIV-1 LTNPs compared with HIV-2 and HD. Similar findings were observed for CCR5+ cells although limited to central-memory CD4 T cell (TCM) and follicular helper T cell subsets, whereas all major subsets from HIV-1 LTNPs contained less CXCR6+ cells compared with HIV-2. All six V3 loop sequences from HIV-1 LTNPs contained a proline at position 326. Proportions of SAMHD1+ cells were higher in all resting CD4 T subsets from HIV-1 LTNPs compared with the other groups, whereas Blimp-1+ and Trim5α+ cells did not differ. The CD4 T cell subsets from HIV-1 LTNPs differ from those of HIV-2-infected subjects by higher levels of activation, exhaustion, and SAMHD1 expression that can reflect the distinct patterns of host/virus relationships.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2021 |
|---|---|
| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:37 |
|---|---|
| Enthalten in: |
AIDS research and human retroviruses - 37(2021), 3 vom: 04. März, Seite 214-223 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Diallo, Mariama Sadjo [VerfasserIn] |
|---|
| Links: |
|---|
| Anmerkungen: |
Date Completed 18.08.2021 Date Revised 31.05.2022 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1089/AID.2020.0084 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM316203629 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM316203629 | ||
| 003 | DE-627 | ||
| 005 | 20231225160557.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2021 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1089/AID.2020.0084 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1053.xml |
| 035 | |a (DE-627)NLM316203629 | ||
| 035 | |a (NLM)33050708 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Diallo, Mariama Sadjo |e verfasserin |4 aut | |
| 245 | 1 | 2 | |a A Comparison of Cell Activation, Exhaustion, and Expression of HIV Coreceptors and Restriction Factors in HIV-1- and HIV-2-Infected Nonprogressors |
| 264 | 1 | |c 2021 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 18.08.2021 | ||
| 500 | |a Date Revised 31.05.2022 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Human immunodeficiency viruses induce rare attenuated diseases due either to HIV-1 in the exceptional long-term nonprogressors (LTNPs) or to HIV-2 in West Africa. To better understand characteristics of these two disease types we performed a multiplex comparative analysis of cell activation, exhaustion, and expression of coreceptors and restriction factors in CD4 T cells susceptible to harbor those viruses. We analyzed by flow cytometry the expression of HLA-DR, PD1, CCR5, CXCR6, SAMHD1, Blimp-1, and TRIM5α on CD4 T cell subsets from 10 HIV-1+ LTNPs and 14 HIV-2+ (12 nonprogressors and 2 progressors) of the ANRS CO-15 and CO-5 cohorts, respectively, and 12 HIV- healthy donors (HD). The V3 loop of the HIV-1 envelope from 6 HIV-1+ LTNPs was sequenced to determine the CXCR6-binding capacity. Proportions of HLA-DR+ and PD1+ cells were higher in memory CD4 T subsets from HIV-1 LTNPs compared with HIV-2 and HD. Similar findings were observed for CCR5+ cells although limited to central-memory CD4 T cell (TCM) and follicular helper T cell subsets, whereas all major subsets from HIV-1 LTNPs contained less CXCR6+ cells compared with HIV-2. All six V3 loop sequences from HIV-1 LTNPs contained a proline at position 326. Proportions of SAMHD1+ cells were higher in all resting CD4 T subsets from HIV-1 LTNPs compared with the other groups, whereas Blimp-1+ and Trim5α+ cells did not differ. The CD4 T cell subsets from HIV-1 LTNPs differ from those of HIV-2-infected subjects by higher levels of activation, exhaustion, and SAMHD1 expression that can reflect the distinct patterns of host/virus relationships | ||
| 650 | 4 | |a Comparative Study | |
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a CD4 T cells | |
| 650 | 4 | |a HIV | |
| 650 | 4 | |a coreceptors | |
| 650 | 4 | |a restriction factors | |
| 650 | 7 | |a Antiviral Restriction Factors |2 NLM | |
| 650 | 7 | |a Tripartite Motif Proteins |2 NLM | |
| 650 | 7 | |a TRIM5 protein, human |2 NLM | |
| 650 | 7 | |a EC 2.3.2.27 |2 NLM | |
| 650 | 7 | |a Ubiquitin-Protein Ligases |2 NLM | |
| 650 | 7 | |a EC 2.3.2.27 |2 NLM | |
| 700 | 1 | |a Samri, Assia |e verfasserin |4 aut | |
| 700 | 1 | |a Charpentier, Charlotte |e verfasserin |4 aut | |
| 700 | 1 | |a Bertine, Mélanie |e verfasserin |4 aut | |
| 700 | 1 | |a Cheynier, Rémi |e verfasserin |4 aut | |
| 700 | 1 | |a Thiébaut, Rodolphe |e verfasserin |4 aut | |
| 700 | 1 | |a Matheron, Sophie |e verfasserin |4 aut | |
| 700 | 1 | |a Collin, Fidéline |e verfasserin |4 aut | |
| 700 | 1 | |a Braibant, Martine |e verfasserin |4 aut | |
| 700 | 1 | |a Candotti, Daniel |e verfasserin |4 aut | |
| 700 | 1 | |a Brun-Vézinet, Françoise |e verfasserin |4 aut | |
| 700 | 1 | |a Autran, Brigitte |e verfasserin |4 aut | |
| 700 | 0 | |a ANRS CO5 IMMUNOVIR-2 and ANRS CO15 ALT Study Groups |e verfasserin |4 aut | |
| 700 | 1 | |a Appay, Victor |e investigator |4 oth | |
| 700 | 1 | |a Autran, Brigitte |e investigator |4 oth | |
| 700 | 1 | |a Brun-Vezinet, Françoise |e investigator |4 oth | |
| 700 | 1 | |a Chaghil, Nathalie |e investigator |4 oth | |
| 700 | 1 | |a Descamps, Diane |e investigator |4 oth | |
| 700 | 1 | |a Hosmalin, Anne |e investigator |4 oth | |
| 700 | 1 | |a Pancino, Gianfranco |e investigator |4 oth | |
| 700 | 1 | |a Manel, Nicolas |e investigator |4 oth | |
| 700 | 1 | |a Marchand, Lucie |e investigator |4 oth | |
| 700 | 1 | |a Pedroza-Martins, Livia |e investigator |4 oth | |
| 700 | 1 | |a Sàez-Cirion, Asier |e investigator |4 oth | |
| 700 | 1 | |a Vieillard, Vincent |e investigator |4 oth | |
| 700 | 1 | |a Agut, Henri |e investigator |4 oth | |
| 700 | 1 | |a Clauvel, Jean-Pierre |e investigator |4 oth | |
| 700 | 1 | |a Costagliola, Dominique |e investigator |4 oth | |
| 700 | 1 | |a Debré, Patrice |e investigator |4 oth | |
| 700 | 1 | |a Theodorou, Ioannis |e investigator |4 oth | |
| 700 | 1 | |a Sicard, Didier |e investigator |4 oth | |
| 700 | 1 | |a Viard, Jean-Paul |e investigator |4 oth | |
| 700 | 1 | |a Barin, Francis |e investigator |4 oth | |
| 700 | 1 | |a Vieillard, Vincent |e investigator |4 oth | |
| 700 | 1 | |a Autran, Brigitte |e investigator |4 oth | |
| 773 | 0 | 8 | |i Enthalten in |t AIDS research and human retroviruses |d 1990 |g 37(2021), 3 vom: 04. März, Seite 214-223 |w (DE-627)NLM012611727 |x 1931-8405 |7 nnns |
| 773 | 1 | 8 | |g volume:37 |g year:2021 |g number:3 |g day:04 |g month:03 |g pages:214-223 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1089/AID.2020.0084 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 37 |j 2021 |e 3 |b 04 |c 03 |h 214-223 | ||