Qualitative and Quantitative Analysis of Ukrainian Iris Species : A Fresh Look on Their Antioxidant Content and Biological Activities
The major groups of antioxidant compounds (isoflavonoids, xanthones, hydroxycinnamic acids) in the rhizome methanol extracts of four Ukrainian Iris sp. (Iris pallida, Iris hungarica, Iris sibirica, and Iris variegata) were qualitatively and quantitatively analyzed using HPLC-DAD and UPLC-MS/MS. Gallic acid, caffeic acid, mangiferin, tectoridin, irigenin, iristectorigenin B, irisolidone, 5,6-dihydroxy-7,8,3',5'-tetramethoxyisoflavone, irisolidone-7-O-β-d-glucopyranoside, germanaism B, and nigricin were recognized by comparing their UV/MS spectra, chromatographic retention time (tR) with those of standard reference compounds. I. hungarica and I. variegata showed the highest total amount of phenolic compounds. Germanaism B was the most abundant component in the rhizomes of I. variegata (7.089 ± 0.032 mg/g) and I. hungarica (6.285 ± 0.030 mg/g). The compound analyses showed good calibration curve linearity (r2 > 0.999) and low detection and quantifications limit. These results validated the method for its use in the simultaneous quantitative evaluation of phenolic compounds in the studied Iris sp. I. hungarica and I. variegata rhizomes exhibited antioxidant activity, as demonstrated by the HPLC-ABTS system and NRF2 expression assay and anti-inflammatory activity on respiratory burst in human neutrophils. Moreover, the extracts showed anti-allergic and cytotoxic effects against cancer cells. Anti-coronavirus 229E and lipid formation activities were also evaluated. In summary, potent antioxidant marker compounds were identified in the examined Iris sp.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:25 |
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| Enthalten in: |
Molecules (Basel, Switzerland) - 25(2020), 19 vom: 08. Okt. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Mykhailenko, Olha [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 26.10.2020 Date Revised 29.10.2020 published: Electronic Citation Status MEDLINE |
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| doi: |
10.3390/molecules25194588 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM31619719X |
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| 100 | 1 | |a Mykhailenko, Olha |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Qualitative and Quantitative Analysis of Ukrainian Iris Species |b A Fresh Look on Their Antioxidant Content and Biological Activities |
| 264 | 1 | |c 2020 | |
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| 500 | |a Date Completed 26.10.2020 | ||
| 500 | |a Date Revised 29.10.2020 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a The major groups of antioxidant compounds (isoflavonoids, xanthones, hydroxycinnamic acids) in the rhizome methanol extracts of four Ukrainian Iris sp. (Iris pallida, Iris hungarica, Iris sibirica, and Iris variegata) were qualitatively and quantitatively analyzed using HPLC-DAD and UPLC-MS/MS. Gallic acid, caffeic acid, mangiferin, tectoridin, irigenin, iristectorigenin B, irisolidone, 5,6-dihydroxy-7,8,3',5'-tetramethoxyisoflavone, irisolidone-7-O-β-d-glucopyranoside, germanaism B, and nigricin were recognized by comparing their UV/MS spectra, chromatographic retention time (tR) with those of standard reference compounds. I. hungarica and I. variegata showed the highest total amount of phenolic compounds. Germanaism B was the most abundant component in the rhizomes of I. variegata (7.089 ± 0.032 mg/g) and I. hungarica (6.285 ± 0.030 mg/g). The compound analyses showed good calibration curve linearity (r2 > 0.999) and low detection and quantifications limit. These results validated the method for its use in the simultaneous quantitative evaluation of phenolic compounds in the studied Iris sp. I. hungarica and I. variegata rhizomes exhibited antioxidant activity, as demonstrated by the HPLC-ABTS system and NRF2 expression assay and anti-inflammatory activity on respiratory burst in human neutrophils. Moreover, the extracts showed anti-allergic and cytotoxic effects against cancer cells. Anti-coronavirus 229E and lipid formation activities were also evaluated. In summary, potent antioxidant marker compounds were identified in the examined Iris sp | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a HPLC-ABTS | |
| 650 | 4 | |a HPLC-DAD | |
| 650 | 4 | |a Iris rhizomes | |
| 650 | 4 | |a UPLC–MS/MS | |
| 650 | 4 | |a anti-allergic | |
| 650 | 4 | |a anti-inflammatory | |
| 650 | 4 | |a antioxidant | |
| 650 | 4 | |a coronavirus 229E | |
| 650 | 4 | |a cytotoxic | |
| 650 | 4 | |a phenolic compounds | |
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| 650 | 7 | |a Antineoplastic Agents |2 NLM | |
| 650 | 7 | |a Antioxidants |2 NLM | |
| 650 | 7 | |a Antiviral Agents |2 NLM | |
| 650 | 7 | |a NF-E2-Related Factor 2 |2 NLM | |
| 650 | 7 | |a NFE2L2 protein, human |2 NLM | |
| 650 | 7 | |a Plant Extracts |2 NLM | |
| 700 | 1 | |a Korinek, Michal |e verfasserin |4 aut | |
| 700 | 1 | |a Ivanauskas, Liudas |e verfasserin |4 aut | |
| 700 | 1 | |a Bezruk, Ivan |e verfasserin |4 aut | |
| 700 | 1 | |a Myhal, Artem |e verfasserin |4 aut | |
| 700 | 1 | |a Petrikaitė, Vilma |e verfasserin |4 aut | |
| 700 | 1 | |a El-Shazly, Mohamed |e verfasserin |4 aut | |
| 700 | 1 | |a Lin, Guan-Hua |e verfasserin |4 aut | |
| 700 | 1 | |a Lin, Chia-Yi |e verfasserin |4 aut | |
| 700 | 1 | |a Yen, Chia-Hung |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Bing-Hung |e verfasserin |4 aut | |
| 700 | 1 | |a Georgiyants, Victoriya |e verfasserin |4 aut | |
| 700 | 1 | |a Hwang, Tsong-Long |e verfasserin |4 aut | |
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