Wolffia globosa-Mankai Plant-Based Protein Contains Bioactive Vitamin B12 and Is Well Absorbed in Humans
BACKGROUND: Rare plants that contain corrinoid compounds mostly comprise cobalamin analogues, which may compete with cobalamin (vitamin B12 (B12)) metabolism. We examined the presence of B12 in a cultivated strain of an aquatic plant: Wolffia globosa (Mankai), and predicted functional pathways using gut-bioreactor, and the effects of long-term Mankai consumption as a partial meat substitute, on serum B12 concentrations.
METHODS: We used microbiological assay, liquid-chromatography/electrospray-ionization-tandem-mass-spectrometry (LC-MS/MS), and anoxic bioreactors for the B12 experiments. We explored the effect of a green Mediterranean/low-meat diet, containing 100 g of frozen Mankai shake/day, on serum B12 levels during the 18-month DIRECT-PLUS (ID:NCT03020186) weight-loss trial, compared with control and Mediterranean diet groups.
RESULTS: The B12 content of Mankai was consistent at different seasons (p = 0.76). Several cobalamin congeners (Hydroxocobalamin(OH-B12); 5-deoxyadenosylcobalamin(Ado-B12); methylcobalamin(Me-B12); cyanocobalamin(CN-B12)) were identified in Mankai extracts, whereas no pseudo B12 was detected. A higher abundance of 16S-rRNA gene amplicon sequences associated with a genome containing a KEGG ortholog involved in microbial B12 metabolism were observed, compared with control bioreactors that lacked Mankai. Following the DIRECT-PLUS intervention (n = 294 participants; retention-rate = 89%; baseline B12 = 420.5 ± 187.8 pg/mL), serum B12 increased by 5.2% in control, 9.9% in Mediterranean, and 15.4% in Mankai-containing green Mediterranean/low-meat diets (p = 0.025 between extreme groups).
CONCLUSIONS: Mankai plant contains bioactive B12 compounds and could serve as a B12 plant-based food source.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2020 |
|---|---|
| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:12 |
|---|---|
| Enthalten in: |
Nutrients - 12(2020), 10 vom: 08. Okt. |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Sela, Ilan [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Flexitarians |
|---|
| Anmerkungen: |
Date Completed 11.05.2021 Date Revised 11.05.2021 published: Electronic Citation Status MEDLINE |
|---|
| doi: |
10.3390/nu12103067 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM316195855 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM316195855 | ||
| 003 | DE-627 | ||
| 005 | 20231225160547.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2020 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.3390/nu12103067 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1053.xml |
| 035 | |a (DE-627)NLM316195855 | ||
| 035 | |a (NLM)33049929 | ||
| 035 | |a (PII)E3067 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Sela, Ilan |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Wolffia globosa-Mankai Plant-Based Protein Contains Bioactive Vitamin B12 and Is Well Absorbed in Humans |
| 264 | 1 | |c 2020 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 11.05.2021 | ||
| 500 | |a Date Revised 11.05.2021 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a BACKGROUND: Rare plants that contain corrinoid compounds mostly comprise cobalamin analogues, which may compete with cobalamin (vitamin B12 (B12)) metabolism. We examined the presence of B12 in a cultivated strain of an aquatic plant: Wolffia globosa (Mankai), and predicted functional pathways using gut-bioreactor, and the effects of long-term Mankai consumption as a partial meat substitute, on serum B12 concentrations | ||
| 520 | |a METHODS: We used microbiological assay, liquid-chromatography/electrospray-ionization-tandem-mass-spectrometry (LC-MS/MS), and anoxic bioreactors for the B12 experiments. We explored the effect of a green Mediterranean/low-meat diet, containing 100 g of frozen Mankai shake/day, on serum B12 levels during the 18-month DIRECT-PLUS (ID:NCT03020186) weight-loss trial, compared with control and Mediterranean diet groups | ||
| 520 | |a RESULTS: The B12 content of Mankai was consistent at different seasons (p = 0.76). Several cobalamin congeners (Hydroxocobalamin(OH-B12); 5-deoxyadenosylcobalamin(Ado-B12); methylcobalamin(Me-B12); cyanocobalamin(CN-B12)) were identified in Mankai extracts, whereas no pseudo B12 was detected. A higher abundance of 16S-rRNA gene amplicon sequences associated with a genome containing a KEGG ortholog involved in microbial B12 metabolism were observed, compared with control bioreactors that lacked Mankai. Following the DIRECT-PLUS intervention (n = 294 participants; retention-rate = 89%; baseline B12 = 420.5 ± 187.8 pg/mL), serum B12 increased by 5.2% in control, 9.9% in Mediterranean, and 15.4% in Mankai-containing green Mediterranean/low-meat diets (p = 0.025 between extreme groups) | ||
| 520 | |a CONCLUSIONS: Mankai plant contains bioactive B12 compounds and could serve as a B12 plant-based food source | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Randomized Controlled Trial | |
| 650 | 4 | |a Wolffia globosa | |
| 650 | 4 | |a flexitarians | |
| 650 | 4 | |a plant-based nutrition | |
| 650 | 4 | |a sustainability | |
| 650 | 4 | |a vitamin B12 | |
| 650 | 4 | |a weight loss | |
| 650 | 7 | |a Plant Extracts |2 NLM | |
| 650 | 7 | |a Plant Proteins |2 NLM | |
| 650 | 7 | |a Vitamin B 12 |2 NLM | |
| 650 | 7 | |a P6YC3EG204 |2 NLM | |
| 700 | 1 | |a Yaskolka Meir, Anat |e verfasserin |4 aut | |
| 700 | 1 | |a Brandis, Alexander |e verfasserin |4 aut | |
| 700 | 1 | |a Krajmalnik-Brown, Rosa |e verfasserin |4 aut | |
| 700 | 1 | |a Zeibich, Lydia |e verfasserin |4 aut | |
| 700 | 1 | |a Chang, Debbie |e verfasserin |4 aut | |
| 700 | 1 | |a Dirks, Blake |e verfasserin |4 aut | |
| 700 | 1 | |a Tsaban, Gal |e verfasserin |4 aut | |
| 700 | 1 | |a Kaplan, Alon |e verfasserin |4 aut | |
| 700 | 1 | |a Rinott, Ehud |e verfasserin |4 aut | |
| 700 | 1 | |a Zelicha, Hila |e verfasserin |4 aut | |
| 700 | 1 | |a Arinos, Shira |e verfasserin |4 aut | |
| 700 | 1 | |a Ceglarek, Uta |e verfasserin |4 aut | |
| 700 | 1 | |a Isermann, Berend |e verfasserin |4 aut | |
| 700 | 1 | |a Lapidot, Miri |e verfasserin |4 aut | |
| 700 | 1 | |a Green, Ralph |e verfasserin |4 aut | |
| 700 | 1 | |a Shai, Iris |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Nutrients |d 2009 |g 12(2020), 10 vom: 08. Okt. |w (DE-627)NLM206140738 |x 2072-6643 |7 nnns |
| 773 | 1 | 8 | |g volume:12 |g year:2020 |g number:10 |g day:08 |g month:10 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.3390/nu12103067 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 12 |j 2020 |e 10 |b 08 |c 10 | ||