Bone-derived dECM/alginate bioink for fabricating a 3D cell-laden mesh structure for bone tissue engineering
Copyright © 2020 Elsevier Ltd. All rights reserved..
Alginate bioink has been widely employed to fabricate 3D cell-laden structures because of its low toxicity, appropriate biocompatibility, and easy/fast cross-linking ability. However, the low bioactivity of the hydrogel is a main shortcoming, so that physical or chemical modification with bioactive components is a promising strategy to efficiently increase the biological activity of alginate hydrogel. The present study proposes a new method to obtain bioactive alginate-based bioink by supplementing it with methacrylated (Ma)-decellularized extracellular matrix (dECM) derived from bone tissues. We demonstrate that the appropriate processing conditions and concentration of Ma-dECM in the bioink offer not only reasonable printability for fabricating 3D cell-laden structures, but also meaningful cell viability of the printed cell-laden construct. Moreover, the biologically improved microenvironment of alginate-based cell-laden structures formed using our method demonstrated a substantial effect on the osteogenic differentiation of the human adipose derived stem cells that were laden in the bioink.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:250 |
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| Enthalten in: |
Carbohydrate polymers - 250(2020) vom: 15. Dez., Seite 116914 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Lee, Jiun [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 01.04.2021 Date Revised 01.04.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.carbpol.2020.116914 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM316194905 |
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| 520 | |a Alginate bioink has been widely employed to fabricate 3D cell-laden structures because of its low toxicity, appropriate biocompatibility, and easy/fast cross-linking ability. However, the low bioactivity of the hydrogel is a main shortcoming, so that physical or chemical modification with bioactive components is a promising strategy to efficiently increase the biological activity of alginate hydrogel. The present study proposes a new method to obtain bioactive alginate-based bioink by supplementing it with methacrylated (Ma)-decellularized extracellular matrix (dECM) derived from bone tissues. We demonstrate that the appropriate processing conditions and concentration of Ma-dECM in the bioink offer not only reasonable printability for fabricating 3D cell-laden structures, but also meaningful cell viability of the printed cell-laden construct. Moreover, the biologically improved microenvironment of alginate-based cell-laden structures formed using our method demonstrated a substantial effect on the osteogenic differentiation of the human adipose derived stem cells that were laden in the bioink | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a 2-Hydroxy-4′-(2-hydroxyethoxy)-2-methylpropiophenone (Irgacure 2959: PubChem CID: 86266) | |
| 650 | 4 | |a Alginate | |
| 650 | 4 | |a bio-printing | |
| 650 | 4 | |a bone tissue engineering | |
| 650 | 4 | |a calcium chloride (PubChem CID: 5284359) | |
| 650 | 4 | |a methacrylated dECM | |
| 650 | 4 | |a methacrylic anhydride (PubChem CID: 12974) | |
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