Structural insights into mRNA reading frame regulation by tRNA modification and slippery codon-anticodon pairing
© 2020, Hoffer et al..
Modifications in the tRNA anticodon loop, adjacent to the three-nucleotide anticodon, influence translation fidelity by stabilizing the tRNA to allow for accurate reading of the mRNA genetic code. One example is the N1-methylguanosine modification at guanine nucleotide 37 (m1G37) located in the anticodon loop andimmediately adjacent to the anticodon nucleotides 34, 35, 36. The absence of m1G37 in tRNAPro causes +1 frameshifting on polynucleotide, slippery codons. Here, we report structures of the bacterial ribosome containing tRNAPro bound to either cognate or slippery codons to determine how the m1G37 modification prevents mRNA frameshifting. The structures reveal that certain codon-anticodon contexts and the lack of m1G37 destabilize interactions of tRNAPro with the P site of the ribosome, causing large conformational changes typically only seen during EF-G-mediated translocation of the mRNA-tRNA pairs. These studies provide molecular insights into how m1G37 stabilizes the interactions of tRNAPro with the ribosome in the context of a slippery mRNA codon.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2020 |
|---|---|
| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:9 |
|---|---|
| Enthalten in: |
eLife - 9(2020) vom: 05. Okt. |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Hoffer, Eric D [VerfasserIn] |
|---|
| Links: |
|---|
| Anmerkungen: |
Date Completed 22.02.2021 Date Revised 22.02.2021 published: Electronic Citation Status MEDLINE |
|---|
| doi: |
10.7554/eLife.51898 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM315871482 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM315871482 | ||
| 003 | DE-627 | ||
| 005 | 20231225155841.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2020 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.7554/eLife.51898 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1052.xml |
| 035 | |a (DE-627)NLM315871482 | ||
| 035 | |a (NLM)33016876 | ||
| 035 | |a (PII)e51898 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Hoffer, Eric D |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Structural insights into mRNA reading frame regulation by tRNA modification and slippery codon-anticodon pairing |
| 264 | 1 | |c 2020 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 22.02.2021 | ||
| 500 | |a Date Revised 22.02.2021 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2020, Hoffer et al. | ||
| 520 | |a Modifications in the tRNA anticodon loop, adjacent to the three-nucleotide anticodon, influence translation fidelity by stabilizing the tRNA to allow for accurate reading of the mRNA genetic code. One example is the N1-methylguanosine modification at guanine nucleotide 37 (m1G37) located in the anticodon loop andimmediately adjacent to the anticodon nucleotides 34, 35, 36. The absence of m1G37 in tRNAPro causes +1 frameshifting on polynucleotide, slippery codons. Here, we report structures of the bacterial ribosome containing tRNAPro bound to either cognate or slippery codons to determine how the m1G37 modification prevents mRNA frameshifting. The structures reveal that certain codon-anticodon contexts and the lack of m1G37 destabilize interactions of tRNAPro with the P site of the ribosome, causing large conformational changes typically only seen during EF-G-mediated translocation of the mRNA-tRNA pairs. These studies provide molecular insights into how m1G37 stabilizes the interactions of tRNAPro with the ribosome in the context of a slippery mRNA codon | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Research Support, U.S. Gov't, Non-P.H.S. | |
| 650 | 4 | |a E. coli | |
| 650 | 4 | |a biochemistry | |
| 650 | 4 | |a chemical biology | |
| 650 | 4 | |a fidelity | |
| 650 | 4 | |a frameshift | |
| 650 | 4 | |a mRNA | |
| 650 | 4 | |a molecular biophysics | |
| 650 | 4 | |a near-cognate | |
| 650 | 4 | |a ribosome | |
| 650 | 4 | |a structural biology | |
| 650 | 4 | |a tRNA | |
| 650 | 7 | |a Anticodon |2 NLM | |
| 650 | 7 | |a Codon |2 NLM | |
| 650 | 7 | |a RNA, Messenger |2 NLM | |
| 650 | 7 | |a RNA, Transfer |2 NLM | |
| 650 | 7 | |a 9014-25-9 |2 NLM | |
| 700 | 1 | |a Hong, Samuel |e verfasserin |4 aut | |
| 700 | 1 | |a Sunita, S |e verfasserin |4 aut | |
| 700 | 1 | |a Maehigashi, Tatsuya |e verfasserin |4 aut | |
| 700 | 1 | |a Gonzalez, Ruben L |c Jnr |e verfasserin |4 aut | |
| 700 | 1 | |a Whitford, Paul C |e verfasserin |4 aut | |
| 700 | 1 | |a Dunham, Christine M |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t eLife |d 2012 |g 9(2020) vom: 05. Okt. |w (DE-627)NLM221831460 |x 2050-084X |7 nnns |
| 773 | 1 | 8 | |g volume:9 |g year:2020 |g day:05 |g month:10 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.7554/eLife.51898 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 9 |j 2020 |b 05 |c 10 | ||