Lensless, reflection-based dark-field microscopy (RDFM) on a CMOS chip
© 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement..
We present for the first time a lens-free, oblique illumination imaging platform for on-sensor dark- field microscopy and shadow-based 3D object measurements. It consists of an LED point source that illuminates a 5-megapixel, 1.4 µm pixel size, back-illuminated CMOS sensor at angles between 0° and 90°. Analytes (polystyrene beads, microorganisms, and cells) were placed and imaged directly onto the sensor. The spatial resolution of this imaging system is limited by the pixel size (∼1.4 µm) over the whole area of the sensor (3.6×2.73 mm). We demonstrated two imaging modalities: (i) shadow imaging for estimation of 3D object dimensions (on polystyrene beads and microorganisms) when the illumination angle is between 0° and 85°, and (ii) dark-field imaging, at >85° illumination angles. In dark-field mode, a 3-4 times drop in background intensity and contrast reversal similar to traditional dark-field imaging was observed, due to larger reflection intensities at those angles. With this modality, we were able to detect and analyze morphological features of bacteria and single-celled algae clusters.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:11 |
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| Enthalten in: |
Biomedical optics express - 11(2020), 9 vom: 01. Sept., Seite 4942-4959 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Imanbekova, Meruyert [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Revised 06.10.2020 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1364/BOE.394615 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM315848766 |
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| 245 | 1 | 0 | |a Lensless, reflection-based dark-field microscopy (RDFM) on a CMOS chip |
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| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a © 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement. | ||
| 520 | |a We present for the first time a lens-free, oblique illumination imaging platform for on-sensor dark- field microscopy and shadow-based 3D object measurements. It consists of an LED point source that illuminates a 5-megapixel, 1.4 µm pixel size, back-illuminated CMOS sensor at angles between 0° and 90°. Analytes (polystyrene beads, microorganisms, and cells) were placed and imaged directly onto the sensor. The spatial resolution of this imaging system is limited by the pixel size (∼1.4 µm) over the whole area of the sensor (3.6×2.73 mm). We demonstrated two imaging modalities: (i) shadow imaging for estimation of 3D object dimensions (on polystyrene beads and microorganisms) when the illumination angle is between 0° and 85°, and (ii) dark-field imaging, at >85° illumination angles. In dark-field mode, a 3-4 times drop in background intensity and contrast reversal similar to traditional dark-field imaging was observed, due to larger reflection intensities at those angles. With this modality, we were able to detect and analyze morphological features of bacteria and single-celled algae clusters | ||
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| 700 | 1 | |a Perumal, Ayyappasamy Sudalaiyadum |e verfasserin |4 aut | |
| 700 | 1 | |a Kheireddine, Sara |e verfasserin |4 aut | |
| 700 | 1 | |a Nicolau, Dan V |e verfasserin |4 aut | |
| 700 | 1 | |a Wachsmann-Hogiu, Sebastian |e verfasserin |4 aut | |
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