Topical lignocaine anaesthesia for oropharyngeal sampling for COVID-19
OBJECTIVES: To ascertain if topical lignocaine application in oropharynx prior to swab sampling to test for COVID-19 improves a patient's comfort and to assess its effect on the swab sample taken to conduct the RT-PCR.
METHODS: Adult patients testing positive on the RT-PCR COVID-19 test were sampled again within 48 h after administering topical oropharyngeal anaesthesia. Patients were asked to rate their discomfort on a visual analog scale (VAS) for both sample A and B. A qualitative real-time RT-PCR for detection of SARS-CoV-2 RNA, was performed, and the cycle threshold value (Ct), used as a surrogate marker for the viral load, was measured for the sample taken without lignocaine (sample A) and the sample taken post-lignocaine application (sample B). The difference in Ct values of both the groups was checked for any statistical significance using paired t-test. Wilcoxon signed rank test was used on VAS scores to determine any significant decrease in discomfort.
RESULTS: Forty patients were included in the study. Twenty-nine patients (72.5%) reported the procedure to be more comfortable post-lignocaine application. Median (IQR) discomfort on VAS decreased from 7 (1) to 5 (2) after lignocaine use, which was statistically significant (p < 0.05). Mean Ct value for sample A was 17.21 ± 5.25 and for sample B was 18.44 ± 4.8 (p > 0.05), indicating a non-significant effect of lignocaine on SARS-CoV-2 concentration in the sample.
CONCLUSION: Topical lignocaine, while improving the comfort of the procedure of oropharyngeal sampling for patient did not alter the SARS-CoV-2 viral load that was detected in nasal and oropharyngeal samples taken together.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2021 |
|---|---|
| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:278 |
|---|---|
| Enthalten in: |
European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery - 278(2021), 5 vom: 01. Mai, Seite 1669-1673 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Kanodia, Anupam [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
98PI200987 |
|---|
| Anmerkungen: |
Date Completed 23.04.2021 Date Revised 23.04.2021 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1007/s00405-020-06402-z |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM315717459 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM315717459 | ||
| 003 | DE-627 | ||
| 005 | 20231225155524.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2021 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s00405-020-06402-z |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1052.xml |
| 035 | |a (DE-627)NLM315717459 | ||
| 035 | |a (NLM)33001294 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Kanodia, Anupam |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Topical lignocaine anaesthesia for oropharyngeal sampling for COVID-19 |
| 264 | 1 | |c 2021 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 23.04.2021 | ||
| 500 | |a Date Revised 23.04.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a OBJECTIVES: To ascertain if topical lignocaine application in oropharynx prior to swab sampling to test for COVID-19 improves a patient's comfort and to assess its effect on the swab sample taken to conduct the RT-PCR | ||
| 520 | |a METHODS: Adult patients testing positive on the RT-PCR COVID-19 test were sampled again within 48 h after administering topical oropharyngeal anaesthesia. Patients were asked to rate their discomfort on a visual analog scale (VAS) for both sample A and B. A qualitative real-time RT-PCR for detection of SARS-CoV-2 RNA, was performed, and the cycle threshold value (Ct), used as a surrogate marker for the viral load, was measured for the sample taken without lignocaine (sample A) and the sample taken post-lignocaine application (sample B). The difference in Ct values of both the groups was checked for any statistical significance using paired t-test. Wilcoxon signed rank test was used on VAS scores to determine any significant decrease in discomfort | ||
| 520 | |a RESULTS: Forty patients were included in the study. Twenty-nine patients (72.5%) reported the procedure to be more comfortable post-lignocaine application. Median (IQR) discomfort on VAS decreased from 7 (1) to 5 (2) after lignocaine use, which was statistically significant (p < 0.05). Mean Ct value for sample A was 17.21 ± 5.25 and for sample B was 18.44 ± 4.8 (p > 0.05), indicating a non-significant effect of lignocaine on SARS-CoV-2 concentration in the sample | ||
| 520 | |a CONCLUSION: Topical lignocaine, while improving the comfort of the procedure of oropharyngeal sampling for patient did not alter the SARS-CoV-2 viral load that was detected in nasal and oropharyngeal samples taken together | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Lignocaine | |
| 650 | 4 | |a RT-PCR | |
| 650 | 4 | |a SARS coronavirus | |
| 650 | 7 | |a RNA, Viral |2 NLM | |
| 650 | 7 | |a Lidocaine |2 NLM | |
| 650 | 7 | |a 98PI200987 |2 NLM | |
| 700 | 1 | |a Srigyan, Deepankar |e verfasserin |4 aut | |
| 700 | 1 | |a Sikka, Kapil |e verfasserin |4 aut | |
| 700 | 1 | |a Choudhary, Aashish |e verfasserin |4 aut | |
| 700 | 1 | |a Choudekar, Avinash |e verfasserin |4 aut | |
| 700 | 1 | |a Mittal, Saurabh |e verfasserin |4 aut | |
| 700 | 1 | |a Bhopale, Shweta Arun |e verfasserin |4 aut | |
| 700 | 1 | |a Dar, Lalit |e verfasserin |4 aut | |
| 700 | 1 | |a Thakar, Alok |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery |d 1994 |g 278(2021), 5 vom: 01. Mai, Seite 1669-1673 |w (DE-627)NLM012637548 |x 1434-4726 |7 nnns |
| 773 | 1 | 8 | |g volume:278 |g year:2021 |g number:5 |g day:01 |g month:05 |g pages:1669-1673 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1007/s00405-020-06402-z |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 278 |j 2021 |e 5 |b 01 |c 05 |h 1669-1673 | ||