Single particle detection of protein molecules using dark-field microscopy to avoid signals from nonspecific adsorption
Copyright © 2020 Elsevier B.V. All rights reserved..
A massively parallel single particle sensing method based on core-satellite formation of Au nanoparticles was introduced for the detection of interleukin 6 (IL-6). This method exploits the fact that the localized plasmon resonance (LSPR) of the plasmonic nanoparticles will change as a result of core-satellite formation, resulting in a change in the observed color. In this method, the hue (color) value of thousands of 67 nm Au nanoparticles immobilized on a glass coverslip surface is analyzed by a Matlab code before and after the addition of reporter nanoparticles containing IL-6 as target protein. The average hue shift as the result of core-satellite formation is used as the basis to detect small amount of proteins. This method enjoys two major advantages. First it is able to analyze the hue values of thousands of nanoparticles in parallel in less than a minute. Secondly the method is able to circumvent the effect of non-specific adsorption, a major issue in the field of biosensing.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:169 |
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| Enthalten in: |
Biosensors & bioelectronics - 169(2020) vom: 01. Dez., Seite 112612 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Markhali, Bijan P [VerfasserIn] |
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| Links: |
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| Themen: |
7440-57-5 |
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| Anmerkungen: |
Date Completed 14.05.2021 Date Revised 14.05.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.bios.2020.112612 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM315478454 |
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| 500 | |a Date Revised 14.05.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2020 Elsevier B.V. All rights reserved. | ||
| 520 | |a A massively parallel single particle sensing method based on core-satellite formation of Au nanoparticles was introduced for the detection of interleukin 6 (IL-6). This method exploits the fact that the localized plasmon resonance (LSPR) of the plasmonic nanoparticles will change as a result of core-satellite formation, resulting in a change in the observed color. In this method, the hue (color) value of thousands of 67 nm Au nanoparticles immobilized on a glass coverslip surface is analyzed by a Matlab code before and after the addition of reporter nanoparticles containing IL-6 as target protein. The average hue shift as the result of core-satellite formation is used as the basis to detect small amount of proteins. This method enjoys two major advantages. First it is able to analyze the hue values of thousands of nanoparticles in parallel in less than a minute. Secondly the method is able to circumvent the effect of non-specific adsorption, a major issue in the field of biosensing | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Antibody | |
| 650 | 4 | |a Dark-field microscopy | |
| 650 | 4 | |a Ensemble detection | |
| 650 | 4 | |a Nonspecific adsorption | |
| 650 | 4 | |a Protein | |
| 650 | 4 | |a Single particle sensing | |
| 650 | 7 | |a Gold |2 NLM | |
| 650 | 7 | |a 7440-57-5 |2 NLM | |
| 700 | 1 | |a Sriram, Manish |e verfasserin |4 aut | |
| 700 | 1 | |a Bennett, Danielle T |e verfasserin |4 aut | |
| 700 | 1 | |a Khiabani, Parisa S |e verfasserin |4 aut | |
| 700 | 1 | |a Hoque, Sharmin |e verfasserin |4 aut | |
| 700 | 1 | |a Tilley, Richard D |e verfasserin |4 aut | |
| 700 | 1 | |a Bakthavathsalam, Padmavathy |e verfasserin |4 aut | |
| 700 | 1 | |a Gooding, J Justin |e verfasserin |4 aut | |
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