Knockdown EIF3C Suppresses Cell Proliferation and Increases Apoptosis in Pancreatic Cancer Cell
© The Author(s) 2020..
Increasing evidence shows that eukaryotic initiation factor subunit (EIF3C) plays a crucial role in development of tumors. However, the underlying roles of EIF3Cin the development of pancreatic cancer (PC) remain unknown. In this study, we examined the expression of EIF3C in PC tissues, their adjacent normal tissues and 3 cell lines (SW1990, PANC-1 and AsPC-1). Moreover, the EIF3C-shRNA lentivirus was constructed to suppress EIF3C expression. Following this, the cell colony formation assay was employed to evaluate proliferation ability of PC cells. Meanwhile, the cell cycle and apoptotic assays were also performed by flow cytometry. We found that level of EIF3C in PC tissues was significantly increased compared with that in adjacent normal tissues. Furthermore, the knockdown of EIF3C can significantly reduce cell proliferation, block cell cycle in G2/M and induce apoptosis in both SW1990 and PANC-1 cells. Our findings suggest that EIF3C plays a crucial role in the progression of PC and may be a potential target in the treatment of PC.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2020 |
|---|---|
| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:18 |
|---|---|
| Enthalten in: |
Dose-response : a publication of International Hormesis Society - 18(2020), 3 vom: 24. Juli, Seite 1559325820950061 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Jiao, Heng [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Eukaryotic initiation factor subunit |
|---|
| Anmerkungen: |
Date Revised 28.09.2020 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
|---|
| doi: |
10.1177/1559325820950061 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM315442034 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM315442034 | ||
| 003 | DE-627 | ||
| 005 | 20231225154925.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2020 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1177/1559325820950061 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1051.xml |
| 035 | |a (DE-627)NLM315442034 | ||
| 035 | |a (NLM)32973416 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Jiao, Heng |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Knockdown EIF3C Suppresses Cell Proliferation and Increases Apoptosis in Pancreatic Cancer Cell |
| 264 | 1 | |c 2020 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Revised 28.09.2020 | ||
| 500 | |a published: Electronic-eCollection | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a © The Author(s) 2020. | ||
| 520 | |a Increasing evidence shows that eukaryotic initiation factor subunit (EIF3C) plays a crucial role in development of tumors. However, the underlying roles of EIF3Cin the development of pancreatic cancer (PC) remain unknown. In this study, we examined the expression of EIF3C in PC tissues, their adjacent normal tissues and 3 cell lines (SW1990, PANC-1 and AsPC-1). Moreover, the EIF3C-shRNA lentivirus was constructed to suppress EIF3C expression. Following this, the cell colony formation assay was employed to evaluate proliferation ability of PC cells. Meanwhile, the cell cycle and apoptotic assays were also performed by flow cytometry. We found that level of EIF3C in PC tissues was significantly increased compared with that in adjacent normal tissues. Furthermore, the knockdown of EIF3C can significantly reduce cell proliferation, block cell cycle in G2/M and induce apoptosis in both SW1990 and PANC-1 cells. Our findings suggest that EIF3C plays a crucial role in the progression of PC and may be a potential target in the treatment of PC | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a PANC-1 cells | |
| 650 | 4 | |a SW1990 cells | |
| 650 | 4 | |a eukaryotic initiation factor subunit | |
| 650 | 4 | |a pancreatic adenocarcinoma | |
| 700 | 1 | |a Zeng, Lingxiao |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Shengsheng |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Jianpeng |e verfasserin |4 aut | |
| 700 | 1 | |a Lou, Wenhui |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Dose-response : a publication of International Hormesis Society |d 2006 |g 18(2020), 3 vom: 24. Juli, Seite 1559325820950061 |w (DE-627)NLM181105713 |x 1559-3258 |7 nnns |
| 773 | 1 | 8 | |g volume:18 |g year:2020 |g number:3 |g day:24 |g month:07 |g pages:1559325820950061 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1177/1559325820950061 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 18 |j 2020 |e 3 |b 24 |c 07 |h 1559325820950061 | ||