Enhanced migration of breast and lung cancer cells deficient for cN-II and CD73 via COX-2/PGE2/AKT axis regulation
PURPOSE: Purine metabolism involves various intracellular and extracellular enzymes, including cN-II and CD73 that dephosphorylate intracellular and extracellular nucleoside monophosphates into their corresponding nucleosides. We conducted a study to better understand the biological roles of these enzymes in breast and lung cancer cells.
METHODS: We modified cN-II and/or CD73 expression in human breast cancer cells (MDA-MB-231), human lung cancer cells (NCI-H292) and murine breast cancer cells (4T1) using the CRISPR/Cas9 technique, and evaluated their impact on various cellular parameters such as proliferation, migration, invasion, intracellular nucleotide pools and nucleotide metabolism-related gene expression under extracellular nucleotide stress conditions.
RESULTS: Intracellular nucleotide contents were found to be altered in the modified cancer cell models both at their basal levels and after exposure to adenosine or AMP. Altered cN-II and CD73 levels were also found to be associated with cell migration and invasion alterations, involving TIMP-2, MMP-2 and MMP-9 expression, as well as alterations in the COX-2/PGE2/AKT pathway.
CONCLUSION: Our results highlight new cell-specific roles of cN-II and CD73 in cancer cell biology and provide insight into their interactions with different intracellular pathways.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:44 |
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| Enthalten in: |
Cellular oncology (Dordrecht) - 44(2021), 1 vom: 24. Feb., Seite 151-165 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Cadassou, Octavia [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 23.09.2021 Date Revised 23.09.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1007/s13402-020-00558-w |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM315411473 |
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| 100 | 1 | |a Cadassou, Octavia |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Enhanced migration of breast and lung cancer cells deficient for cN-II and CD73 via COX-2/PGE2/AKT axis regulation |
| 264 | 1 | |c 2021 | |
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| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
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| 500 | |a Date Completed 23.09.2021 | ||
| 500 | |a Date Revised 23.09.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a PURPOSE: Purine metabolism involves various intracellular and extracellular enzymes, including cN-II and CD73 that dephosphorylate intracellular and extracellular nucleoside monophosphates into their corresponding nucleosides. We conducted a study to better understand the biological roles of these enzymes in breast and lung cancer cells | ||
| 520 | |a METHODS: We modified cN-II and/or CD73 expression in human breast cancer cells (MDA-MB-231), human lung cancer cells (NCI-H292) and murine breast cancer cells (4T1) using the CRISPR/Cas9 technique, and evaluated their impact on various cellular parameters such as proliferation, migration, invasion, intracellular nucleotide pools and nucleotide metabolism-related gene expression under extracellular nucleotide stress conditions | ||
| 520 | |a RESULTS: Intracellular nucleotide contents were found to be altered in the modified cancer cell models both at their basal levels and after exposure to adenosine or AMP. Altered cN-II and CD73 levels were also found to be associated with cell migration and invasion alterations, involving TIMP-2, MMP-2 and MMP-9 expression, as well as alterations in the COX-2/PGE2/AKT pathway | ||
| 520 | |a CONCLUSION: Our results highlight new cell-specific roles of cN-II and CD73 in cancer cell biology and provide insight into their interactions with different intracellular pathways | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Breast cancer | |
| 650 | 4 | |a CD73 | |
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| 650 | 4 | |a Cancer cell biology | |
| 650 | 4 | |a Lung cancer | |
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| 700 | 1 | |a Machon, Christelle |e verfasserin |4 aut | |
| 700 | 1 | |a Gudefin, Laura |e verfasserin |4 aut | |
| 700 | 1 | |a Armanet, Célia |e verfasserin |4 aut | |
| 700 | 1 | |a Chettab, Kamel |e verfasserin |4 aut | |
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| 700 | 1 | |a Tozzi, Maria Grazia |e verfasserin |4 aut | |
| 700 | 1 | |a Dumontet, Charles |e verfasserin |4 aut | |
| 700 | 1 | |a Cros-Perrial, Emeline |e verfasserin |4 aut | |
| 700 | 1 | |a Jordheim, Lars Petter |e verfasserin |4 aut | |
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