High-intensity ultrasound irradiation promotes the release of extracellular vesicles from C2C12 myotubes
Copyright © 2020 Elsevier B.V. All rights reserved..
Skeletal muscle is an important secretory organ in mammals, producing myriad chemical mediators ("myokines") with distinct biological action in different tissues, including anti-inflammatory activity. Extracellular vesicles (EVs) have recently been identified as a mode of myokine transport from muscle, facilitating such anti-inflammatory activity. In this report, we have demonstrated that high-intensity ultrasound (US) strongly induces EV secretion from cultured myotubes without a reduction in cell viability. High-intensity US of 3.0 W/cm2 with 20% duty cycle increased the number of EVs by 2-fold compared to control at 6 h. This effect was specific to EVs in the 100-150 nm size range. Thus, high-intensity US is a novel modality for inducing myocellular EV release and may hold therapeutic value.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:110 |
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| Enthalten in: |
Ultrasonics - 110(2021) vom: 15. Feb., Seite 106243 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Maeshige, Noriaki [VerfasserIn] |
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| Links: |
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| Themen: |
Extracellular vesicles |
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| Anmerkungen: |
Date Completed 04.01.2021 Date Revised 04.01.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.ultras.2020.106243 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM315325860 |
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| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2020 Elsevier B.V. All rights reserved. | ||
| 520 | |a Skeletal muscle is an important secretory organ in mammals, producing myriad chemical mediators ("myokines") with distinct biological action in different tissues, including anti-inflammatory activity. Extracellular vesicles (EVs) have recently been identified as a mode of myokine transport from muscle, facilitating such anti-inflammatory activity. In this report, we have demonstrated that high-intensity ultrasound (US) strongly induces EV secretion from cultured myotubes without a reduction in cell viability. High-intensity US of 3.0 W/cm2 with 20% duty cycle increased the number of EVs by 2-fold compared to control at 6 h. This effect was specific to EVs in the 100-150 nm size range. Thus, high-intensity US is a novel modality for inducing myocellular EV release and may hold therapeutic value | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Extracellular vesicles | |
| 650 | 4 | |a High-intensity ultrasound | |
| 650 | 4 | |a Myotubes | |
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| 700 | 1 | |a Yuan, Zhi-Min |e verfasserin |4 aut | |
| 700 | 1 | |a Kondo, Hiroyo |e verfasserin |4 aut | |
| 700 | 1 | |a Fujino, Hidemi |e verfasserin |4 aut | |
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