Detection of SARS-CoV-2 in conjunctival secretions from patients without ocular symptoms
PURPOSE: To evaluate the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in conjunctival secretions from patients without ocular symptoms.
METHODS: Conjunctival swabs were prospectively collected from laboratory-confirmed Coronavirus disease 2019 (COVID-19) patients without ocular symptoms for reverse transcription-polymerase chain reaction (RT-PCR) and viral culture.
RESULTS: A total of 158 conjunctival swabs were obtained from 49 laboratory-confirmed COVID-19 patients. The median duration of illness when the first conjunctival swab was obtained was 10 days (range 2-27 days). Four conjunctival swabs from four different patients (4/49, 8.2%) were positive for SARS-CoV-2 RNA by RT-PCR. The Ct values ranged from 32.7 to 37.7 (mean 35.4). Viral cultures were negative for all four RT-PCR-positive conjunctival swabs.
CONCLUSION: Conjunctival secretions of a minority of COVID-19 patients without ocular symptoms may contain relatively low levels of SARS-CoV-2 RNA, but their infectiousness remains undetermined. Appropriate infection control measures should be implemented during ophthalmological assessment of COVID-19 patients to prevent potential nosocomial transmission of SARS-CoV-2.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:49 |
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| Enthalten in: |
Infection - 49(2021), 2 vom: 17. Apr., Seite 257-265 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Li, Xin [VerfasserIn] |
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| Links: |
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| Themen: |
Asymptomatic |
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| Anmerkungen: |
Date Completed 06.04.2021 Date Revised 06.04.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1007/s15010-020-01524-2 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM315163194 |
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| 100 | 1 | |a Li, Xin |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Detection of SARS-CoV-2 in conjunctival secretions from patients without ocular symptoms |
| 264 | 1 | |c 2021 | |
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| 500 | |a Date Completed 06.04.2021 | ||
| 500 | |a Date Revised 06.04.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a PURPOSE: To evaluate the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in conjunctival secretions from patients without ocular symptoms | ||
| 520 | |a METHODS: Conjunctival swabs were prospectively collected from laboratory-confirmed Coronavirus disease 2019 (COVID-19) patients without ocular symptoms for reverse transcription-polymerase chain reaction (RT-PCR) and viral culture | ||
| 520 | |a RESULTS: A total of 158 conjunctival swabs were obtained from 49 laboratory-confirmed COVID-19 patients. The median duration of illness when the first conjunctival swab was obtained was 10 days (range 2-27 days). Four conjunctival swabs from four different patients (4/49, 8.2%) were positive for SARS-CoV-2 RNA by RT-PCR. The Ct values ranged from 32.7 to 37.7 (mean 35.4). Viral cultures were negative for all four RT-PCR-positive conjunctival swabs | ||
| 520 | |a CONCLUSION: Conjunctival secretions of a minority of COVID-19 patients without ocular symptoms may contain relatively low levels of SARS-CoV-2 RNA, but their infectiousness remains undetermined. Appropriate infection control measures should be implemented during ophthalmological assessment of COVID-19 patients to prevent potential nosocomial transmission of SARS-CoV-2 | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a Conjunctiva | |
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| 700 | 1 | |a Li, Kenneth Kai-Wang |e verfasserin |4 aut | |
| 700 | 1 | |a Tso, Eugene Yuk-Keung |e verfasserin |4 aut | |
| 700 | 1 | |a Yip, Cyril Chik-Yan |e verfasserin |4 aut | |
| 700 | 1 | |a Sridhar, Siddharth |e verfasserin |4 aut | |
| 700 | 1 | |a Chung, Tom Wai-Hin |e verfasserin |4 aut | |
| 700 | 1 | |a Chiu, Kelvin Hei-Yeung |e verfasserin |4 aut | |
| 700 | 1 | |a Hung, Derek Ling-Lung |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Alan Ka-Lun |e verfasserin |4 aut | |
| 700 | 1 | |a Chau, Sandy Ka-Yee |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Raymond |e verfasserin |4 aut | |
| 700 | 1 | |a Lung, Kwok-Cheung |e verfasserin |4 aut | |
| 700 | 1 | |a Tam, Anthony Raymond |e verfasserin |4 aut | |
| 700 | 1 | |a Cheng, Vincent Chi-Chung |e verfasserin |4 aut | |
| 700 | 1 | |a To, Kelvin Kai-Wang |e verfasserin |4 aut | |
| 700 | 1 | |a Chan, Kwok-Hung |e verfasserin |4 aut | |
| 700 | 1 | |a Hung, Ivan Fan-Ngai |e verfasserin |4 aut | |
| 700 | 1 | |a Yuen, Kwok-Yung |e verfasserin |4 aut | |
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