Immobilization of hemoglobin on MnCO3 sphere-loaded Au nanoparticles as highly efficient sensing platform towards hydrogen peroxide
In this paper, we report the synthesis of MnCO3-Au hybrid microspheres and their application on the electrochemical biosensing of hydrogen peroxide (H2O2) based on the immobilization of hemoglobin (Hb). The characterization of MnCO3-Au microspheres revealed that an abundance of Au nanoparticles (AuNPs) has been absorbed on the surface of the spherical MnCO3 by the electrostatic assembly. The combined unique properties of MnCO3-Au microspheres are beneficial for the realization of the direct electron transfer of Hb. Hb immobilized on the microspheres maintained its biological activity, showing a surface-controlled process with the heterogeneous electron transfer rate constant (k s) of 2.63 s-1. The fabricated biosensor displayed an excellent performance for the electrocatalytic reduction of H2O2. The linear range for the determination of H2O2 was from 0.06-40.0 μM with a detection limit of 0.015 µM (S/N = 3). The biosensor also exhibited high selectivity, good repeatability and long-term stability, which offers great potential for H2O2 detection in real sample analysis.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:32 |
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| Enthalten in: |
Nanotechnology - 32(2021), 2 vom: 08. Jan., Seite 025503 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Xu, Linyi [VerfasserIn] |
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| Links: |
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| Themen: |
059QF0KO0R |
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| Anmerkungen: |
Date Completed 04.06.2021 Date Revised 18.01.2022 published: Print Citation Status MEDLINE |
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| doi: |
10.1088/1361-6528/abb8a5 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM315041544 |
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| 520 | |a In this paper, we report the synthesis of MnCO3-Au hybrid microspheres and their application on the electrochemical biosensing of hydrogen peroxide (H2O2) based on the immobilization of hemoglobin (Hb). The characterization of MnCO3-Au microspheres revealed that an abundance of Au nanoparticles (AuNPs) has been absorbed on the surface of the spherical MnCO3 by the electrostatic assembly. The combined unique properties of MnCO3-Au microspheres are beneficial for the realization of the direct electron transfer of Hb. Hb immobilized on the microspheres maintained its biological activity, showing a surface-controlled process with the heterogeneous electron transfer rate constant (k s) of 2.63 s-1. The fabricated biosensor displayed an excellent performance for the electrocatalytic reduction of H2O2. The linear range for the determination of H2O2 was from 0.06-40.0 μM with a detection limit of 0.015 µM (S/N = 3). The biosensor also exhibited high selectivity, good repeatability and long-term stability, which offers great potential for H2O2 detection in real sample analysis | ||
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| 650 | 7 | |a Immobilized Proteins |2 NLM | |
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| 700 | 1 | |a Shi, Weishan |e verfasserin |4 aut | |
| 700 | 1 | |a Bao, Ning |e verfasserin |4 aut | |
| 700 | 1 | |a Yu, Chunmei |e verfasserin |4 aut | |
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