Alu-repeat polymorphism in the tissue plasminogen activator (t-PA) gene, seminal t-PA concentration, and male fertility impairment : A case-control study
Copyright © 2020 Tahtamouni et al..
BACKGROUND: Tissue plasminogen activator (t-PA) is a protein involved in the fibrinolytic system that catalyzes the conversion of plasminogen into the active plasmin. The activity of t-PA is controlled by plasminogen activator inhibitor-1. t-PA has crucial functions during spermatogenesis. One polymorphism was reported for t-PA gene, either the presence of a 300-bp Alu-repeat (Alu + ) or its absence (Alu - ).
OBJECTIVE: The current work aimed at studying the association between Alu polymorphism in the t-PA gene and male infertility.
MATERIALS AND METHODS: Using polymerase chain reaction on genomic DNA isolated from the blood of 79 participants, a region polymorphic for Alu element insertion in t-PA gene was amplified. In addition, total t-PA concentration, plasminogen activator inhibitor-1 /t-PA complex concentration, and t-PA activity in seminal plasma were measured by enzyme-linked immunosorbent assay.
RESULTS: The results indicate that the percentage of infertile participants (n = 50) who were homozygous for t-PA Alu insertion (Alu + / + ), heterozygous Alu + / - or homozygous for t-PA Alu deletion (Alu - / - ) did not change significantly (p = 0.43, 0.81, and 0.85, respectively) when compared with the control participants (n = 29). On the other hand, a significant decrease (p = 0.0001) of t-PA total concentration in seminal plasma was observed in the infertile group in comparison with the control group. However, the results indicate that there is no association between the t-PA Alu different genotypes and the total t-PA seminal concentration in the infertile group when compared to the control group (p = 0.63).
CONCLUSION: Data obtained from the current study does not support an association between t-PA Alu polymorphism and t-PA seminal concentration or male infertility.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:18 |
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| Enthalten in: |
International journal of reproductive biomedicine - 18(2020), 8 vom: 21. Aug., Seite 571-578 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Tahtamouni, Lubna Hamid [VerfasserIn] |
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| Links: |
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| Themen: |
Alu element |
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| Anmerkungen: |
Date Revised 16.04.2022 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
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| doi: |
10.18502/ijrm.v13i8.7496 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM315026693 |
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| 100 | 1 | |a Tahtamouni, Lubna Hamid |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Alu-repeat polymorphism in the tissue plasminogen activator (t-PA) gene, seminal t-PA concentration, and male fertility impairment |b A case-control study |
| 264 | 1 | |c 2020 | |
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| 500 | |a Date Revised 16.04.2022 | ||
| 500 | |a published: Electronic-eCollection | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a Copyright © 2020 Tahtamouni et al. | ||
| 520 | |a BACKGROUND: Tissue plasminogen activator (t-PA) is a protein involved in the fibrinolytic system that catalyzes the conversion of plasminogen into the active plasmin. The activity of t-PA is controlled by plasminogen activator inhibitor-1. t-PA has crucial functions during spermatogenesis. One polymorphism was reported for t-PA gene, either the presence of a 300-bp Alu-repeat (Alu + ) or its absence (Alu - ) | ||
| 520 | |a OBJECTIVE: The current work aimed at studying the association between Alu polymorphism in the t-PA gene and male infertility | ||
| 520 | |a MATERIALS AND METHODS: Using polymerase chain reaction on genomic DNA isolated from the blood of 79 participants, a region polymorphic for Alu element insertion in t-PA gene was amplified. In addition, total t-PA concentration, plasminogen activator inhibitor-1 /t-PA complex concentration, and t-PA activity in seminal plasma were measured by enzyme-linked immunosorbent assay | ||
| 520 | |a RESULTS: The results indicate that the percentage of infertile participants (n = 50) who were homozygous for t-PA Alu insertion (Alu + / + ), heterozygous Alu + / - or homozygous for t-PA Alu deletion (Alu - / - ) did not change significantly (p = 0.43, 0.81, and 0.85, respectively) when compared with the control participants (n = 29). On the other hand, a significant decrease (p = 0.0001) of t-PA total concentration in seminal plasma was observed in the infertile group in comparison with the control group. However, the results indicate that there is no association between the t-PA Alu different genotypes and the total t-PA seminal concentration in the infertile group when compared to the control group (p = 0.63) | ||
| 520 | |a CONCLUSION: Data obtained from the current study does not support an association between t-PA Alu polymorphism and t-PA seminal concentration or male infertility | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Male infertility | |
| 650 | 4 | |a Semen | |
| 650 | 4 | |a Spermatogenesis | |
| 650 | 4 | |a t-PA. | |
| 650 | 4 | |a Alu element | |
| 700 | 1 | |a Hamdan, Mahmoud Nael |e verfasserin |4 aut | |
| 700 | 1 | |a Al-Mazaydeh, Zainab Ali |e verfasserin |4 aut | |
| 700 | 1 | |a Bawadi, Randa Mahmoud |e verfasserin |4 aut | |
| 700 | 1 | |a Rammaha, Majdoleen Sobhi |e verfasserin |4 aut | |
| 700 | 1 | |a Zghoul, Ahmad Mohammad |e verfasserin |4 aut | |
| 700 | 1 | |a Ahram, Mamoun Ahmad |e verfasserin |4 aut | |
| 700 | 1 | |a Yasin, Salem Refat |e verfasserin |4 aut | |
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