A thermophilic chitinase 1602 from the marine bacterium Microbulbifer sp. BN3 and its high-level expression in Pichia pastoris
© 2020 International Union of Biochemistry and Molecular Biology, Inc..
Chitinases play an important role in many industrial processes, including the preparation of oligosaccharides with potential applications. In the present study, a 1,713 bp gene of Chi1602, derived from a marine bacterium Microbulbifer sp. BN3, encoding a GH18 family chitinase, was expressed at high levels in Pichia pastoris. Distinct from most of the marine chitinases, the recombinant chitinase 1602 exhibited maximal activity at 60 °C and over a broad pH range between 5.0 and 9.0, and was stable at 50 °C and over the pH range 4.0-9.0. The hydrolytic products derived from colloidal chitins comprised mainly (GlcNAc)2 and GlcNAc, indicating that rChi1602 is a GH18 processive chitinase in conformity with its hypothetical structure. However, rChi1602 showed traces of β-N-acetylglucosaminidase activity on substrates such as powder chitin, chitosan, and ethylene glycol chitin. The thermophilic rChi1602, which manifests adaptation to a wide pH range and can be expressed at a high level in P. pastoris, is advantageous for applications in industrial processes.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:68 |
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| Enthalten in: |
Biotechnology and applied biochemistry - 68(2021), 5 vom: 10. Okt., Seite 1076-1085 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Li, Ren Kuan [VerfasserIn] |
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| Links: |
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| Themen: |
Chitinases |
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| Anmerkungen: |
Date Completed 29.12.2021 Date Revised 29.12.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1002/bab.2027 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM314962700 |
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| 245 | 1 | 2 | |a A thermophilic chitinase 1602 from the marine bacterium Microbulbifer sp. BN3 and its high-level expression in Pichia pastoris |
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| 500 | |a Date Revised 29.12.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2020 International Union of Biochemistry and Molecular Biology, Inc. | ||
| 520 | |a Chitinases play an important role in many industrial processes, including the preparation of oligosaccharides with potential applications. In the present study, a 1,713 bp gene of Chi1602, derived from a marine bacterium Microbulbifer sp. BN3, encoding a GH18 family chitinase, was expressed at high levels in Pichia pastoris. Distinct from most of the marine chitinases, the recombinant chitinase 1602 exhibited maximal activity at 60 °C and over a broad pH range between 5.0 and 9.0, and was stable at 50 °C and over the pH range 4.0-9.0. The hydrolytic products derived from colloidal chitins comprised mainly (GlcNAc)2 and GlcNAc, indicating that rChi1602 is a GH18 processive chitinase in conformity with its hypothetical structure. However, rChi1602 showed traces of β-N-acetylglucosaminidase activity on substrates such as powder chitin, chitosan, and ethylene glycol chitin. The thermophilic rChi1602, which manifests adaptation to a wide pH range and can be expressed at a high level in P. pastoris, is advantageous for applications in industrial processes | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Microbulbifer sp.BN3 | |
| 650 | 4 | |a Pichia pastoris | |
| 650 | 4 | |a rChi1602 | |
| 650 | 4 | |a thermophilic chitinase | |
| 650 | 7 | |a Chitinases |2 NLM | |
| 650 | 7 | |a EC 3.2.1.14 |2 NLM | |
| 700 | 1 | |a Hu, Ya Juan |e verfasserin |4 aut | |
| 700 | 1 | |a He, Yu Jie |e verfasserin |4 aut | |
| 700 | 1 | |a Ng, Tzi Bun |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Zhi Min |e verfasserin |4 aut | |
| 700 | 1 | |a Ye, Xiu Yun |e verfasserin |4 aut | |
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