A CYC/TB1-type TCP transcription factor controls spikelet meristem identity in barley
© The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com..
Barley possesses a branchless, spike-shaped inflorescence where determinate spikelets attach directly to the main axis, but the developmental mechanism of spikelet identity remains largely unknown. Here we report the functional analysis of the barley gene BRANCHED AND INDETERMINATE SPIKELET 1 (BDI1), which encodes a TCP transcription factor and plays a crucial role in determining barley inflorescence architecture and spikelet development. The bdi1 mutant exhibited indeterminate spikelet meristems that continued to grow and differentiate after producing a floret meristem; some spikelet meristems at the base of the spike formed two fully developed seeds or converted to branched spikelets, producing a branched inflorescence. Map-based cloning analysis showed that this mutant has a deletion of ~600 kb on chromosome 5H containing three putative genes. Expression analysis and virus-induced gene silencing confirmed that the causative gene, BDI1, encodes a CYC/TB1-type TCP transcription factor and is highly conserved in both wild and cultivated barley. Transcriptome and regulatory network analysis demonstrated that BDI1 may integrate regulation of gene transcription cell wall modification and known trehalose-6-phosphate homeostasis to control spikelet development. Together, our findings reveal that BDI1 represents a key regulator of inflorescence architecture and meristem determinacy in cereal crop plants.
| Errataetall: |
CommentIn: J Exp Bot. 2020 Dec 31;71(22):6869-6871. - PMID 33382898 |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:71 |
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| Enthalten in: |
Journal of experimental botany - 71(2020), 22 vom: 31. Dez., Seite 7118-7131 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Shang, Yi [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 14.05.2021 Date Revised 14.05.2021 published: Print CommentIn: J Exp Bot. 2020 Dec 31;71(22):6869-6871. - PMID 33382898 Citation Status MEDLINE |
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| doi: |
10.1093/jxb/eraa416 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM314881433 |
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| 100 | 1 | |a Shang, Yi |e verfasserin |4 aut | |
| 245 | 1 | 2 | |a A CYC/TB1-type TCP transcription factor controls spikelet meristem identity in barley |
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| 500 | |a Date Revised 14.05.2021 | ||
| 500 | |a published: Print | ||
| 500 | |a CommentIn: J Exp Bot. 2020 Dec 31;71(22):6869-6871. - PMID 33382898 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com. | ||
| 520 | |a Barley possesses a branchless, spike-shaped inflorescence where determinate spikelets attach directly to the main axis, but the developmental mechanism of spikelet identity remains largely unknown. Here we report the functional analysis of the barley gene BRANCHED AND INDETERMINATE SPIKELET 1 (BDI1), which encodes a TCP transcription factor and plays a crucial role in determining barley inflorescence architecture and spikelet development. The bdi1 mutant exhibited indeterminate spikelet meristems that continued to grow and differentiate after producing a floret meristem; some spikelet meristems at the base of the spike formed two fully developed seeds or converted to branched spikelets, producing a branched inflorescence. Map-based cloning analysis showed that this mutant has a deletion of ~600 kb on chromosome 5H containing three putative genes. Expression analysis and virus-induced gene silencing confirmed that the causative gene, BDI1, encodes a CYC/TB1-type TCP transcription factor and is highly conserved in both wild and cultivated barley. Transcriptome and regulatory network analysis demonstrated that BDI1 may integrate regulation of gene transcription cell wall modification and known trehalose-6-phosphate homeostasis to control spikelet development. Together, our findings reveal that BDI1 represents a key regulator of inflorescence architecture and meristem determinacy in cereal crop plants | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a BRANCHED AND INDETERMINATE SPIKELET 1 (BDI1) | |
| 650 | 4 | |a Barley | |
| 650 | 4 | |a TCP transcription factor | |
| 650 | 4 | |a deletion | |
| 650 | 4 | |a determinacy | |
| 650 | 4 | |a inflorescence architecture | |
| 650 | 4 | |a map-based cloning | |
| 650 | 4 | |a spikelet meristem | |
| 650 | 4 | |a transcriptome sequencing (RNA-Seq) | |
| 650 | 4 | |a virus-induced gene silencing (VIGS) | |
| 650 | 7 | |a Plant Proteins |2 NLM | |
| 650 | 7 | |a Transcription Factors |2 NLM | |
| 700 | 1 | |a Yuan, Lu |e verfasserin |4 aut | |
| 700 | 1 | |a Di, Zhaocan |e verfasserin |4 aut | |
| 700 | 1 | |a Jia, Yong |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Zhenlan |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Sujuan |e verfasserin |4 aut | |
| 700 | 1 | |a Xing, Liping |e verfasserin |4 aut | |
| 700 | 1 | |a Qi, Zengjun |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Xiaoyun |e verfasserin |4 aut | |
| 700 | 1 | |a Zhu, Jinghuan |e verfasserin |4 aut | |
| 700 | 1 | |a Hua, Wei |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Xiaojian |e verfasserin |4 aut | |
| 700 | 1 | |a Zhu, Minqiu |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Gang |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Chengdao |e verfasserin |4 aut | |
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