Protective Effects of Phellinus linteus Mycelium on the Development of Osteoarthritis after Monosodium Iodoacetate Injection
Copyright © 2020 Mi-Rae Shin et al..
OBJECTIVE: The aim of this study was to identify the protective effects of Phellinus linteus mycelium (PLM) and its possible mechanisms in a model of monosodium iodoacetate- (MIA-) induced osteoarthritis (OA).
METHODS: Intra-articular injection of MIA was injected to 50 μL with 80 mg/mL using a 0.3 mL insulin syringe into the right knee joint. Changes in hindpaw weight-bearing distribution between the right (osteoarthritic) and left (contralateral control) legs were used as an index of joint discomfort. PLM (50, 100, and 200 mg/kg body weight) was orally administered once daily for 14 days from day 7 after MIA treatment. And then, various factors associated with inflammatory response and cartilage degeneration in cartilage tissues detected by western blotting.
RESULTS: PLM treatment showed a concentration-dependent elevation in change in hindpaw weight-bearing distribution (HWBD). PLM200 demonstrated the capacity to significantly increase HWBD, indicating that the change in weight-bearing distribution means the reduction of spontaneous pain. Our results indicate that PLM suppressed the inflammatory factors via NF-κB signaling pathway induced by p38 phosporlyation. Moreover, PLM200 exhibited a significant reduction of ROS produced by the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase. PLM100 and PLM200 inhibited the levels of matrix metalloproteinase (MMP)-1, one of proteinase that degrades extracellular matrix (ECM).
CONCLUSIONS: Taken together, our results indicated that PLM has a strong chondroprotective effect through the suppression both ROS production and inflammation.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:2020 |
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| Enthalten in: |
Evidence-based complementary and alternative medicine : eCAM - 2020(2020) vom: 15., Seite 7240858 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Shin, Mi-Rae [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Revised 16.04.2022 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1155/2020/7240858 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM314808051 |
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| 245 | 1 | 0 | |a Protective Effects of Phellinus linteus Mycelium on the Development of Osteoarthritis after Monosodium Iodoacetate Injection |
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| 500 | |a Date Revised 16.04.2022 | ||
| 500 | |a published: Electronic-eCollection | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a Copyright © 2020 Mi-Rae Shin et al. | ||
| 520 | |a OBJECTIVE: The aim of this study was to identify the protective effects of Phellinus linteus mycelium (PLM) and its possible mechanisms in a model of monosodium iodoacetate- (MIA-) induced osteoarthritis (OA) | ||
| 520 | |a METHODS: Intra-articular injection of MIA was injected to 50 μL with 80 mg/mL using a 0.3 mL insulin syringe into the right knee joint. Changes in hindpaw weight-bearing distribution between the right (osteoarthritic) and left (contralateral control) legs were used as an index of joint discomfort. PLM (50, 100, and 200 mg/kg body weight) was orally administered once daily for 14 days from day 7 after MIA treatment. And then, various factors associated with inflammatory response and cartilage degeneration in cartilage tissues detected by western blotting | ||
| 520 | |a RESULTS: PLM treatment showed a concentration-dependent elevation in change in hindpaw weight-bearing distribution (HWBD). PLM200 demonstrated the capacity to significantly increase HWBD, indicating that the change in weight-bearing distribution means the reduction of spontaneous pain. Our results indicate that PLM suppressed the inflammatory factors via NF-κB signaling pathway induced by p38 phosporlyation. Moreover, PLM200 exhibited a significant reduction of ROS produced by the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase. PLM100 and PLM200 inhibited the levels of matrix metalloproteinase (MMP)-1, one of proteinase that degrades extracellular matrix (ECM) | ||
| 520 | |a CONCLUSIONS: Taken together, our results indicated that PLM has a strong chondroprotective effect through the suppression both ROS production and inflammation | ||
| 650 | 4 | |a Journal Article | |
| 700 | 1 | |a Lee, Jin A |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Min Ju |e verfasserin |4 aut | |
| 700 | 1 | |a Park, Hae-Jin |e verfasserin |4 aut | |
| 700 | 1 | |a Park, Byeong Wook |e verfasserin |4 aut | |
| 700 | 1 | |a Seo, Seung Bo |e verfasserin |4 aut | |
| 700 | 1 | |a Roh, Seong-Soo |e verfasserin |4 aut | |
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