After Experimental Trypanosoma cruzi Infection, Dying Hepatic CD3+TCRαβ+B220+ T Lymphocytes Are Rescued from Death by Peripheral T Cells and Become Activated
The unusual phenotype of CD3+ T lymphocyte expressing B220, a marker originally attributed to B lymphocytes, was first observed in the liver of Fas/Fas-L-deficient mice as a marker of apoptotic T lymphocytes. However, other CD3+B220+ T lymphocyte populations were later described in the periphery as functional cytotoxic or regulatory cells, for example. Then, in this work, we studied whether hepatic CD3+B220+ T lymphocytes could play a role in experimental Trypanosoma cruzi infection. In control and infected mice, we observed two subpopulations that could be discerned based on CD117 expression, which were conventional apoptotic CD3+B220+(CD117-) and thymus-independent CD3+B220+CD117+ T lymphocytes. Regardless of CD117 expression, most B220+ T lymphocytes were 7AAD+, confirming this molecule as a marker of dying T cells. However, after infection, we found that around 15% of the CD3+B220+CD117+ hepatic population became B220 and 7AAD negative, turned into CD90.2+, and upregulated the expression of CD44, CD49d, and CD11a, a phenotype consistent with activated T lymphocytes. Moreover, we observed that the hepatic CD3+B220+CD117+ population was rescued from death by previously activated peripheral T lymphocytes. Our results extend the comprehension of the hepatic CD3+B220+ T lymphocyte subpopulations and illustrate the complex interactions that occur in the liver.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:9 |
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| Enthalten in: |
Pathogens (Basel, Switzerland) - 9(2020), 9 vom: 31. Aug. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Vacani-Martins, Natalia [VerfasserIn] |
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| Links: |
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| Themen: |
CD3+B220+ T lymphocytes |
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| Anmerkungen: |
Date Revised 30.10.2020 published: Electronic Citation Status PubMed-not-MEDLINE |
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| doi: |
10.3390/pathogens9090717 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM314510397 |
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| 245 | 1 | 0 | |a After Experimental Trypanosoma cruzi Infection, Dying Hepatic CD3+TCRαβ+B220+ T Lymphocytes Are Rescued from Death by Peripheral T Cells and Become Activated |
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| 520 | |a The unusual phenotype of CD3+ T lymphocyte expressing B220, a marker originally attributed to B lymphocytes, was first observed in the liver of Fas/Fas-L-deficient mice as a marker of apoptotic T lymphocytes. However, other CD3+B220+ T lymphocyte populations were later described in the periphery as functional cytotoxic or regulatory cells, for example. Then, in this work, we studied whether hepatic CD3+B220+ T lymphocytes could play a role in experimental Trypanosoma cruzi infection. In control and infected mice, we observed two subpopulations that could be discerned based on CD117 expression, which were conventional apoptotic CD3+B220+(CD117-) and thymus-independent CD3+B220+CD117+ T lymphocytes. Regardless of CD117 expression, most B220+ T lymphocytes were 7AAD+, confirming this molecule as a marker of dying T cells. However, after infection, we found that around 15% of the CD3+B220+CD117+ hepatic population became B220 and 7AAD negative, turned into CD90.2+, and upregulated the expression of CD44, CD49d, and CD11a, a phenotype consistent with activated T lymphocytes. Moreover, we observed that the hepatic CD3+B220+CD117+ population was rescued from death by previously activated peripheral T lymphocytes. Our results extend the comprehension of the hepatic CD3+B220+ T lymphocyte subpopulations and illustrate the complex interactions that occur in the liver | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a CD3+B220+ T lymphocytes | |
| 650 | 4 | |a Trypanosoma cruzi infection | |
| 650 | 4 | |a cell death | |
| 650 | 4 | |a hepatic T lymphocytes | |
| 650 | 4 | |a liver immune response | |
| 700 | 1 | |a Meuser-Batista, Marcelo |e verfasserin |4 aut | |
| 700 | 1 | |a Moreira, Otacilio C |e verfasserin |4 aut | |
| 700 | 1 | |a Cascabulho, Cynthia Machado |e verfasserin |4 aut | |
| 700 | 1 | |a Gois Beghini, Daniela |e verfasserin |4 aut | |
| 700 | 1 | |a Horita, Samuel Iwao |e verfasserin |4 aut | |
| 700 | 1 | |a Batista, Marcos Meuser |e verfasserin |4 aut | |
| 700 | 1 | |a Freitas, Fernando Cleber |e verfasserin |4 aut | |
| 700 | 1 | |a Guimarães, Juliana Rodrigues |e verfasserin |4 aut | |
| 700 | 1 | |a Henriques-Pons, Andrea |e verfasserin |4 aut | |
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