Frame-Hydrogel Methodology for Engineering Highly Functional Cardiac Tissue Constructs
Engineered cardiac tissues hold tremendous promise for in vitro drug discovery, studies of heart development and disease, and therapeutic applications. Here, we describe a versatile "frame-hydrogel" methodology to generate engineered cardiac tissues with highly mature functional properties. This methodology has been successfully utilized with a variety of cell sources (neonatal rat ventricular myocytes, human and mouse pluripotent stem cell-derived cardiomyocytes) to generate tissues with diverse 3D geometries (patch, bundle, network) and levels of structural and functional anisotropy. Maturation of such engineered cardiac tissues is rapidly achieved without the need for exogenous electrical or mechanical stimulation or use of complex bioreactors, with tissues routinely reaching conduction velocities and specific forces of 25 cm/s and 20 mN/mm2, respectively, and forces per input cardiomyocyte of up to 12 nN. This method is reproducible and readily scalable to generate small tissues ideal for in vitro testing as well as tissues with large, clinically relevant dimensions.
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2021 |
---|---|
Erschienen: |
2021 |
Enthalten in: |
Zur Gesamtaufnahme - volume:2158 |
---|---|
Enthalten in: |
Methods in molecular biology (Clifton, N.J.) - 2158(2021) vom: 28., Seite 171-186 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Helfer, Abbigail [VerfasserIn] |
---|
Links: |
---|
Anmerkungen: |
Date Completed 12.03.2021 Date Revised 02.01.2022 published: Print Citation Status MEDLINE |
---|
doi: |
10.1007/978-1-0716-0668-1_13 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
NLM314305777 |
---|
LEADER | 01000naa a22002652 4500 | ||
---|---|---|---|
001 | NLM314305777 | ||
003 | DE-627 | ||
005 | 20231225152438.0 | ||
007 | cr uuu---uuuuu | ||
008 | 231225s2021 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.1007/978-1-0716-0668-1_13 |2 doi | |
028 | 5 | 2 | |a pubmed24n1047.xml |
035 | |a (DE-627)NLM314305777 | ||
035 | |a (NLM)32857373 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Helfer, Abbigail |e verfasserin |4 aut | |
245 | 1 | 0 | |a Frame-Hydrogel Methodology for Engineering Highly Functional Cardiac Tissue Constructs |
264 | 1 | |c 2021 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Completed 12.03.2021 | ||
500 | |a Date Revised 02.01.2022 | ||
500 | |a published: Print | ||
500 | |a Citation Status MEDLINE | ||
520 | |a Engineered cardiac tissues hold tremendous promise for in vitro drug discovery, studies of heart development and disease, and therapeutic applications. Here, we describe a versatile "frame-hydrogel" methodology to generate engineered cardiac tissues with highly mature functional properties. This methodology has been successfully utilized with a variety of cell sources (neonatal rat ventricular myocytes, human and mouse pluripotent stem cell-derived cardiomyocytes) to generate tissues with diverse 3D geometries (patch, bundle, network) and levels of structural and functional anisotropy. Maturation of such engineered cardiac tissues is rapidly achieved without the need for exogenous electrical or mechanical stimulation or use of complex bioreactors, with tissues routinely reaching conduction velocities and specific forces of 25 cm/s and 20 mN/mm2, respectively, and forces per input cardiomyocyte of up to 12 nN. This method is reproducible and readily scalable to generate small tissues ideal for in vitro testing as well as tissues with large, clinically relevant dimensions | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Research Support, N.I.H., Extramural | |
650 | 4 | |a Research Support, Non-U.S. Gov't | |
650 | 4 | |a Research Support, U.S. Gov't, Non-P.H.S. | |
650 | 4 | |a Cardiac bundle | |
650 | 4 | |a Cardiac patch | |
650 | 4 | |a Cardiomyocytes | |
650 | 4 | |a Engineered cardiac tissues | |
650 | 4 | |a Human pluripotent stem cells | |
650 | 4 | |a Hydrogel | |
650 | 4 | |a Tissue engineering | |
650 | 7 | |a Hydrogels |2 NLM | |
700 | 1 | |a Bursac, Nenad |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Methods in molecular biology (Clifton, N.J.) |d 1984 |g 2158(2021) vom: 28., Seite 171-186 |w (DE-627)NLM074849794 |x 1940-6029 |7 nnns |
773 | 1 | 8 | |g volume:2158 |g year:2021 |g day:28 |g pages:171-186 |
856 | 4 | 0 | |u http://dx.doi.org/10.1007/978-1-0716-0668-1_13 |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 2158 |j 2021 |b 28 |h 171-186 |