Protein Determination with Molecularly Imprinted Polymer Recognition Combined with Birefringence Liquid Crystal Detection
Liquid crystal-based sensors offer the advantage of high sensitivity at a low cost. However, they often lack selectivity altogether or require costly and unstable biomaterials to impart this selectivity. To incur this selectivity, we herein integrated a molecularly imprinted polymer (MIP) film recognition unit with a liquid crystal (LC) in an optical cell transducer. We tested the resulting chemosensor for protein determination. We examined two different LCs, each with a different optical birefringence. That way, we revealed the influence of that parameter on the sensitivity of the (human serum albumin)-templated (MIP-HSA) LC chemosensor. The response of this chemosensor with the (MIP-HSA)-recognizing film was linear from 2.2 to 15.2 µM HSA, with a limit of detection of 2.2 µM. These values are sufficient to use the devised chemosensor for HSA determination in biological samples. Importantly, the imprinting factor (IF) of this chemosensor was appreciable, reaching IF = 3.7. This IF value indicated the predominant binding of the HSA through specific rather than nonspecific interactions with the MIP.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:20 |
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| Enthalten in: |
Sensors (Basel, Switzerland) - 20(2020), 17 vom: 20. Aug. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Cieplak, Maciej [VerfasserIn] |
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| Links: |
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| Themen: |
Human serum albumin |
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| Anmerkungen: |
Date Completed 26.03.2021 Date Revised 26.03.2021 published: Electronic Citation Status MEDLINE |
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| doi: |
10.3390/s20174692 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM31398994X |
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| 500 | |a Date Revised 26.03.2021 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Liquid crystal-based sensors offer the advantage of high sensitivity at a low cost. However, they often lack selectivity altogether or require costly and unstable biomaterials to impart this selectivity. To incur this selectivity, we herein integrated a molecularly imprinted polymer (MIP) film recognition unit with a liquid crystal (LC) in an optical cell transducer. We tested the resulting chemosensor for protein determination. We examined two different LCs, each with a different optical birefringence. That way, we revealed the influence of that parameter on the sensitivity of the (human serum albumin)-templated (MIP-HSA) LC chemosensor. The response of this chemosensor with the (MIP-HSA)-recognizing film was linear from 2.2 to 15.2 µM HSA, with a limit of detection of 2.2 µM. These values are sufficient to use the devised chemosensor for HSA determination in biological samples. Importantly, the imprinting factor (IF) of this chemosensor was appreciable, reaching IF = 3.7. This IF value indicated the predominant binding of the HSA through specific rather than nonspecific interactions with the MIP | ||
| 650 | 4 | |a Letter | |
| 650 | 4 | |a human serum albumin | |
| 650 | 4 | |a liquid crystal | |
| 650 | 4 | |a molecularly imprinted polymer | |
| 650 | 7 | |a Molecularly Imprinted Polymers |2 NLM | |
| 650 | 7 | |a Proteins |2 NLM | |
| 650 | 7 | |a Serum Albumin, Human |2 NLM | |
| 650 | 7 | |a ZIF514RVZR |2 NLM | |
| 700 | 1 | |a Węgłowski, Rafał |e verfasserin |4 aut | |
| 700 | 1 | |a Iskierko, Zofia |e verfasserin |4 aut | |
| 700 | 1 | |a Węgłowska, Dorota |e verfasserin |4 aut | |
| 700 | 1 | |a Sharma, Piyush S |e verfasserin |4 aut | |
| 700 | 1 | |a Noworyta, Krzysztof R |e verfasserin |4 aut | |
| 700 | 1 | |a D'Souza, Francis |e verfasserin |4 aut | |
| 700 | 1 | |a Kutner, Wlodzimierz |e verfasserin |4 aut | |
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| 856 | 4 | 0 | |u http://dx.doi.org/10.3390/s20174692 |3 Volltext |
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