Chloride intracellular channel 4 is dysregulated in endometrium of women with infertility and alters receptivity
Copyright © 2020 Elsevier Inc. All rights reserved..
The endometrium remodels in each menstrual cycle to become receptive in preparation for embryo implantation which occurs in the mid-secretory phase of the cycle. Failure of blastocyst adhesion and implantation cause infertility. We compared chloride intracellular channel 4 (CLIC4) expression in human endometrium from women with normal fertility and primary unexplained infertility in the mid-secretory/receptive phase of the menstrual cycle. CLIC4 localised to both the epithelial and stromal regions of the endometrium of fertile tissues across the cycle. CLIC4 expression was significantly reduced in the luminal and glandular epithelium and remained unchanged in the stromal region of mid-secretory infertile endometrium compared to fertile endometrium. siRNA knockdown of CLIC4 significantly compromised adhesive capacity of Ishikawa cells (endometrial epithelial cell line). This reduced adhesion and CLIC4 expression was associated with elevated SGK1, p53, SIRT1, BCL2 and MCL1 gene expression in the Ishikawa cells. CLIC4 expression was increased in primary human endometrial stromal cells during decidualization, however, siRNA knockdown of CLIC4 did not affect decidualization. Our data provide evidence that CLIC4 may regulate receptivity and facilitate blastocyst attachment initiating implantation. Reduced CLIC4 levels may be causative of implantation failure in women.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2020 |
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Erschienen: |
2020 |
Enthalten in: |
Zur Gesamtaufnahme - volume:531 |
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Enthalten in: |
Biochemical and biophysical research communications - 531(2020), 4 vom: 22. Okt., Seite 490-496 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Ling, Cheuk Kwan [VerfasserIn] |
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Links: |
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Themen: |
CLIC4 |
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Anmerkungen: |
Date Completed 19.03.2021 Date Revised 19.03.2021 published: Print-Electronic Citation Status MEDLINE |
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doi: |
10.1016/j.bbrc.2020.07.046 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM313820554 |
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520 | |a Copyright © 2020 Elsevier Inc. All rights reserved. | ||
520 | |a The endometrium remodels in each menstrual cycle to become receptive in preparation for embryo implantation which occurs in the mid-secretory phase of the cycle. Failure of blastocyst adhesion and implantation cause infertility. We compared chloride intracellular channel 4 (CLIC4) expression in human endometrium from women with normal fertility and primary unexplained infertility in the mid-secretory/receptive phase of the menstrual cycle. CLIC4 localised to both the epithelial and stromal regions of the endometrium of fertile tissues across the cycle. CLIC4 expression was significantly reduced in the luminal and glandular epithelium and remained unchanged in the stromal region of mid-secretory infertile endometrium compared to fertile endometrium. siRNA knockdown of CLIC4 significantly compromised adhesive capacity of Ishikawa cells (endometrial epithelial cell line). This reduced adhesion and CLIC4 expression was associated with elevated SGK1, p53, SIRT1, BCL2 and MCL1 gene expression in the Ishikawa cells. CLIC4 expression was increased in primary human endometrial stromal cells during decidualization, however, siRNA knockdown of CLIC4 did not affect decidualization. Our data provide evidence that CLIC4 may regulate receptivity and facilitate blastocyst attachment initiating implantation. Reduced CLIC4 levels may be causative of implantation failure in women | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Research Support, Non-U.S. Gov't | |
650 | 4 | |a CLIC4 | |
650 | 4 | |a Cell adhesion | |
650 | 4 | |a Embryo implantation | |
650 | 4 | |a Endometrial receptivity | |
650 | 4 | |a Infertility | |
650 | 4 | |a Uterus | |
650 | 7 | |a CLIC4 protein, human |2 NLM | |
650 | 7 | |a Chloride Channels |2 NLM | |
700 | 1 | |a Santos, Leilani L |e verfasserin |4 aut | |
700 | 1 | |a Zhou, Wei |e verfasserin |4 aut | |
700 | 1 | |a Dimitriadis, Evdokia |e verfasserin |4 aut | |
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