Capsular polysaccharide and lipopolysaccharide O type analysis of Klebsiella pneumoniae isolates by genotype in China
Klebsiella pneumoniae is a common pathogen associated with nosocomial infections and is characterised serologically by capsular polysaccharide (K) and lipopolysaccharide O antigens. We surveyed a total of 348 non-duplicate K. pneumoniae clinical isolates collected over a 1-year period in a tertiary care hospital, and determined their O and K serotypes by sequencing of the wbb Y and wzi gene loci, respectively. Isolates were also screened for antimicrobial resistance and hypervirulent phenotypes; 94 (27.0%) were identified as carbapenem-resistant (CRKP) and 110 (31.6%) as hypervirulent (hvKP). isolates fell into 58 K, and six O types, with 92.0% and 94.2% typeability, respectively. The predominant K types were K14K64 (16.38%), K1 (14.66%), K2 (8.05%) and K57 (5.46%), while O1 (46%), O2a (27.9%) and O3 (11.8%) were the most common. CRKP and hvKP strains had different serotype distributions with O2a:K14K64 (41.0%) being the most frequent among CRKP, and O1:K1 (26.4%) and O1:K2 (17.3%) among hvKP strains. Serotyping by gene sequencing proved to be a useful tool to inform the clinical epidemiology of K. pneumoniae infections and provides valuable data relevant to vaccine design.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:148 |
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| Enthalten in: |
Epidemiology and infection - 148(2020) vom: 12. Aug., Seite e191 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zhang, Z Y [VerfasserIn] |
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| Links: |
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| Themen: |
Antibiotic resistance |
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| Anmerkungen: |
Date Completed 30.10.2020 Date Revised 30.10.2020 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1017/S0950268820001788 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM313568898 |
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| 245 | 1 | 0 | |a Capsular polysaccharide and lipopolysaccharide O type analysis of Klebsiella pneumoniae isolates by genotype in China |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Klebsiella pneumoniae is a common pathogen associated with nosocomial infections and is characterised serologically by capsular polysaccharide (K) and lipopolysaccharide O antigens. We surveyed a total of 348 non-duplicate K. pneumoniae clinical isolates collected over a 1-year period in a tertiary care hospital, and determined their O and K serotypes by sequencing of the wbb Y and wzi gene loci, respectively. Isolates were also screened for antimicrobial resistance and hypervirulent phenotypes; 94 (27.0%) were identified as carbapenem-resistant (CRKP) and 110 (31.6%) as hypervirulent (hvKP). isolates fell into 58 K, and six O types, with 92.0% and 94.2% typeability, respectively. The predominant K types were K14K64 (16.38%), K1 (14.66%), K2 (8.05%) and K57 (5.46%), while O1 (46%), O2a (27.9%) and O3 (11.8%) were the most common. CRKP and hvKP strains had different serotype distributions with O2a:K14K64 (41.0%) being the most frequent among CRKP, and O1:K1 (26.4%) and O1:K2 (17.3%) among hvKP strains. Serotyping by gene sequencing proved to be a useful tool to inform the clinical epidemiology of K. pneumoniae infections and provides valuable data relevant to vaccine design | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Antibiotic resistance | |
| 650 | 4 | |a Klebsiella pneumoniae | |
| 650 | 4 | |a capsular polysaccharide | |
| 650 | 4 | |a genotype | |
| 650 | 4 | |a lipopolysaccharide O type | |
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| 650 | 7 | |a Polysaccharides |2 NLM | |
| 700 | 1 | |a Qin, R |e verfasserin |4 aut | |
| 700 | 1 | |a Lu, Y H |e verfasserin |4 aut | |
| 700 | 1 | |a Shen, J |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, S Y |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, C Y |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Y Q |e verfasserin |4 aut | |
| 700 | 1 | |a Hu, F P |e verfasserin |4 aut | |
| 700 | 1 | |a He, P |e verfasserin |4 aut | |
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