Factor Xa and thrombin induce endothelial progenitor cell activation. The effect of direct oral anticoagulants

Factor Xa (FXa) and thrombin exert non-hemostatic cellular actions primarily mediated through protease-activated receptors (PARs). We investigated the effect of FXa and thrombin on human late-outgrowth endothelial cells (OECs), a type of endothelial progenitor cells (EPCs), and on human umbilical vein endothelial cells (HUVECs). The effect of direct oral anticoagulants (DOACs), rivaroxaban and dabigatran, was also studied. The membrane expression of intercellular adhesion molecule-1 (ICAM-1) and the secretion of monocyte chemoattractant protein-1 (MCP-1) were used as cell activation markers. FXa and thrombin increase the ICAM-1 expression and the MCP-1 secretion on both cells, being higher on OECs. Vorapaxar, a specific PAR-1 antagonist, completely inhibits FXa-induced activation of both cells and thrombin-induced HUVEC activation, but only partially thrombin-induced OEC activation. Furthermore, thrombin-receptor activating peptide; TRAP-6, only partially activates OECs. OECs do not membrane-express PAR-4, therefore it may not be involved on thrombin-induced OEC activation. Rivaroxaban and dabigatran inhibit OEC and HUVEC activation by FXa and thrombin, respectively. Rivaroxaban enhances thrombin-induced OEC and HUVEC activation, which is completely inhibited by vorapaxar. The inhibition of OEC and HUVEC activation by vorapaxar and DOACs may represent a new pleiotropic effect of these drugs. The pathophysiological and clinical significance of our findings need to be established.

Medienart:

E-Artikel

Erscheinungsjahr:

2021

Erschienen:

2021

Enthalten in:

Zur Gesamtaufnahme - volume:32

Enthalten in:

Platelets - 32(2021), 6 vom: 18. Aug., Seite 807-814

Sprache:

Englisch

Beteiligte Personen:

Papadaki, Styliani [VerfasserIn]
Sidiropoulou, Sofia [VerfasserIn]
Moschonas, Iraklis C [VerfasserIn]
Tselepis, Alexandros D [VerfasserIn]

Links:

Volltext

Themen:

Anticoagulants
Dabigatran
EC 3.4.21.5
EC 3.4.21.6
Endothelial progenitor cells
Factor Xa
Journal Article
Rivaroxaban
Thrombin
Vorapaxar

Anmerkungen:

Date Completed 28.12.2021

Date Revised 28.12.2021

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1080/09537104.2020.1802413

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM313376557