Clustering, morphology, and treatment resistance of Bacillus globigii spores recovered from a pilot-scale activated sludge system
Published by Elsevier Ltd..
This study examines the organization and morphology of Bacillus globigii (BG) spores, a common surrogate for Bacillus anthracis, which were seeded and then recovered at various times from several points within a conventional, pilot-scale activated sludge system. Recovered BG spores were enumerated, microscopically examined, and tested for resistance to chemical (i.e. 5% H2O2 for 8 min), thermal (80 °C for 30 min), and ultraviolet light (8 W, 254 nm UV for 1 min) inactivation. Spores exposed to activated sludge germinated, sporulated, and exhibited unique multilayer clustering patterns and statistically significant changes (p < 0.005) in dimensional morphology. Spores collected in the later experimental stages (i.e., during weeks 6 and 7) were significantly more resistant (p ≤ 0.05) to inactivation than those collected on the first day of testing. These results have direct consequences for sludge treatment requirements at wastewater treatment plants that receive spore-containing waste streams.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:260 |
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| Enthalten in: |
Chemosphere - 260(2020) vom: 15. Dez., Seite 127591 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Xing, Yun [VerfasserIn] |
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| Links: |
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| Themen: |
Activated sludge |
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| Anmerkungen: |
Date Completed 15.10.2020 Date Revised 03.12.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.chemosphere.2020.127591 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a This study examines the organization and morphology of Bacillus globigii (BG) spores, a common surrogate for Bacillus anthracis, which were seeded and then recovered at various times from several points within a conventional, pilot-scale activated sludge system. Recovered BG spores were enumerated, microscopically examined, and tested for resistance to chemical (i.e. 5% H2O2 for 8 min), thermal (80 °C for 30 min), and ultraviolet light (8 W, 254 nm UV for 1 min) inactivation. Spores exposed to activated sludge germinated, sporulated, and exhibited unique multilayer clustering patterns and statistically significant changes (p < 0.005) in dimensional morphology. Spores collected in the later experimental stages (i.e., during weeks 6 and 7) were significantly more resistant (p ≤ 0.05) to inactivation than those collected on the first day of testing. These results have direct consequences for sludge treatment requirements at wastewater treatment plants that receive spore-containing waste streams | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Activated sludge | |
| 650 | 4 | |a Atomic force microscopy | |
| 650 | 4 | |a Bacillus globigii | |
| 650 | 4 | |a Inactivation | |
| 650 | 4 | |a Spore resistance | |
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