Quinovic acid purified from medicinal plant Fagonia indica mediates anticancer effects via death receptor 5
Plants are major source for discovery and development of anticancer drugs. Several plant-based anticancer drugs are currently in clinical use. Fagonia indica is a plant of medicinal value in the South Asian countries. Using mass spectrometry and NMR spectroscopy, several compounds were purified from the F. indica extract. We have used one of the purified compounds quinovic acid (QA) and found that QA strongly suppressed the growth and viability of human breast and lung cancer cells. QA did not inhibit growth and viability of non-tumorigenic breast cells. QA mediated its anticancer effects by inducing cell death. QA-induced cell death was associated with biochemical features of apoptosis such as activation of caspases 3 and 8 as well as PARP cleavage. QA also upregulated mRNA and protein levels of death receptor 5 (DR5). Further investigation revealed that QA did not alter DR5 gene promoter activity, but enhanced DR5 mRNA and protein stabilities. DR5 is one of the major components of the extrinsic pathway of apoptosis. Accordingly, Apo2L/TRAIL, the DR5 ligand, potentiated the anticancer effects of QA. Our results indicate that QA mediates its anticancer effects, at least in part, by engaging DR5-depentent pathway to induce apoptosis. Based on our results, we propose that QA in combination with Apo2L/TRAIL can be further investigated as a novel therapeutic approach for breast and lung cancers.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2020 |
|---|---|
| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:474 |
|---|---|
| Enthalten in: |
Molecular and cellular biochemistry - 474(2020), 1-2 vom: 30. Nov., Seite 159-169 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Khayam, Asma Umer [VerfasserIn] |
|---|
| Links: |
|---|
| Anmerkungen: |
Date Completed 17.05.2021 Date Revised 17.05.2021 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1007/s11010-020-03841-4 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM313100527 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM313100527 | ||
| 003 | DE-627 | ||
| 005 | 20231225145836.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2020 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s11010-020-03841-4 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1043.xml |
| 035 | |a (DE-627)NLM313100527 | ||
| 035 | |a (NLM)32734538 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Khayam, Asma Umer |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Quinovic acid purified from medicinal plant Fagonia indica mediates anticancer effects via death receptor 5 |
| 264 | 1 | |c 2020 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 17.05.2021 | ||
| 500 | |a Date Revised 17.05.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Plants are major source for discovery and development of anticancer drugs. Several plant-based anticancer drugs are currently in clinical use. Fagonia indica is a plant of medicinal value in the South Asian countries. Using mass spectrometry and NMR spectroscopy, several compounds were purified from the F. indica extract. We have used one of the purified compounds quinovic acid (QA) and found that QA strongly suppressed the growth and viability of human breast and lung cancer cells. QA did not inhibit growth and viability of non-tumorigenic breast cells. QA mediated its anticancer effects by inducing cell death. QA-induced cell death was associated with biochemical features of apoptosis such as activation of caspases 3 and 8 as well as PARP cleavage. QA also upregulated mRNA and protein levels of death receptor 5 (DR5). Further investigation revealed that QA did not alter DR5 gene promoter activity, but enhanced DR5 mRNA and protein stabilities. DR5 is one of the major components of the extrinsic pathway of apoptosis. Accordingly, Apo2L/TRAIL, the DR5 ligand, potentiated the anticancer effects of QA. Our results indicate that QA mediates its anticancer effects, at least in part, by engaging DR5-depentent pathway to induce apoptosis. Based on our results, we propose that QA in combination with Apo2L/TRAIL can be further investigated as a novel therapeutic approach for breast and lung cancers | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Apo2L/TRAIL | |
| 650 | 4 | |a Breast cancer | |
| 650 | 4 | |a DR5 | |
| 650 | 4 | |a Lung cancer | |
| 650 | 4 | |a Natural product | |
| 650 | 4 | |a Quinovic acid | |
| 650 | 7 | |a Antineoplastic Agents, Phytogenic |2 NLM | |
| 650 | 7 | |a Plant Extracts |2 NLM | |
| 650 | 7 | |a Receptors, TNF-Related Apoptosis-Inducing Ligand |2 NLM | |
| 650 | 7 | |a TNFRSF10B protein, human |2 NLM | |
| 650 | 7 | |a Triterpenes |2 NLM | |
| 650 | 7 | |a quinovic acid |2 NLM | |
| 650 | 7 | |a 9JP167T0ZN |2 NLM | |
| 700 | 1 | |a Patel, Harsh |e verfasserin |4 aut | |
| 700 | 1 | |a Faiola, Nicholas A |e verfasserin |4 aut | |
| 700 | 1 | |a Figueroa Milla, Andre E |e verfasserin |4 aut | |
| 700 | 1 | |a Dilshad, Erum |e verfasserin |4 aut | |
| 700 | 1 | |a Mirza, Bushra |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Ying |e verfasserin |4 aut | |
| 700 | 1 | |a Sheikh, M Saeed |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Molecular and cellular biochemistry |d 1973 |g 474(2020), 1-2 vom: 30. Nov., Seite 159-169 |w (DE-627)NLM000008133 |x 1573-4919 |7 nnns |
| 773 | 1 | 8 | |g volume:474 |g year:2020 |g number:1-2 |g day:30 |g month:11 |g pages:159-169 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1007/s11010-020-03841-4 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 474 |j 2020 |e 1-2 |b 30 |c 11 |h 159-169 | ||