Simultaneous Viral Whole-Genome Sequencing and Differential Expression Profiling in Respiratory Syncytial Virus Infection of Infants
© The Author(s) 2020. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissionsoup.com..
Targeted metagenomics using strand-specific libraries with target enrichment is a sensitive, generalized approach to pathogen sequencing and transcriptome profiling. Using this method, we recovered 13 (76%) complete human respiratory syncytial virus (RSV) genomes from 17 clinical respiratory samples, reconstructed the phylogeny of the infecting viruses, and detected differential gene expression between 2 RSV subgroups, specifically, a lower expression of the P gene and a higher expression of the M2 gene in RSV-A than in RSV-B. This methodology can help to relate viral genetics to clinical phenotype and facilitate ongoing population-level RSV surveillance and vaccine development. Clinical Trials Registration. NCT03627572 and NCT03756766.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:222 |
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| Enthalten in: |
The Journal of infectious diseases - 222(2020), Suppl 7 vom: 07. Okt., Seite S666-S671 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Lin, Gu-Lung [VerfasserIn] |
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| Links: |
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| Themen: |
Differential gene expression |
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| Anmerkungen: |
Date Completed 15.03.2021 Date Revised 17.03.2021 published: Print ClinicalTrials.gov: NCT03627572, NCT03756766 Citation Status MEDLINE |
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| doi: |
10.1093/infdis/jiaa448 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM312781407 |
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| 245 | 1 | 0 | |a Simultaneous Viral Whole-Genome Sequencing and Differential Expression Profiling in Respiratory Syncytial Virus Infection of Infants |
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| 500 | |a Date Revised 17.03.2021 | ||
| 500 | |a published: Print | ||
| 500 | |a ClinicalTrials.gov: NCT03627572, NCT03756766 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © The Author(s) 2020. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissionsoup.com. | ||
| 520 | |a Targeted metagenomics using strand-specific libraries with target enrichment is a sensitive, generalized approach to pathogen sequencing and transcriptome profiling. Using this method, we recovered 13 (76%) complete human respiratory syncytial virus (RSV) genomes from 17 clinical respiratory samples, reconstructed the phylogeny of the infecting viruses, and detected differential gene expression between 2 RSV subgroups, specifically, a lower expression of the P gene and a higher expression of the M2 gene in RSV-A than in RSV-B. This methodology can help to relate viral genetics to clinical phenotype and facilitate ongoing population-level RSV surveillance and vaccine development. Clinical Trials Registration. NCT03627572 and NCT03756766 | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a RNA sequencing | |
| 650 | 4 | |a differential gene expression | |
| 650 | 4 | |a respiratory syncytial virus | |
| 650 | 4 | |a transcriptome profiling | |
| 650 | 4 | |a whole-genome sequencing | |
| 700 | 1 | |a Golubchik, Tanya |e verfasserin |4 aut | |
| 700 | 1 | |a Drysdale, Simon |e verfasserin |4 aut | |
| 700 | 1 | |a O'Connor, Daniel |e verfasserin |4 aut | |
| 700 | 1 | |a Jefferies, Kimberley |e verfasserin |4 aut | |
| 700 | 1 | |a Brown, Anthony |e verfasserin |4 aut | |
| 700 | 1 | |a de Cesare, Mariateresa |e verfasserin |4 aut | |
| 700 | 1 | |a Bonsall, David |e verfasserin |4 aut | |
| 700 | 1 | |a Ansari, M Azim |e verfasserin |4 aut | |
| 700 | 1 | |a Aerssens, Jeroen |e verfasserin |4 aut | |
| 700 | 1 | |a Bont, Louis |e verfasserin |4 aut | |
| 700 | 1 | |a Openshaw, Peter |e verfasserin |4 aut | |
| 700 | 1 | |a Martinón-Torres, Federico |e verfasserin |4 aut | |
| 700 | 1 | |a Bowden, Rory |e verfasserin |4 aut | |
| 700 | 1 | |a Pollard, Andrew J |e verfasserin |4 aut | |
| 700 | 0 | |a RESCEU Investigators |e verfasserin |4 aut | |
| 700 | 1 | |a Nair, Harish |e investigator |4 oth | |
| 700 | 1 | |a Campbell, Harry |e investigator |4 oth | |
| 700 | 1 | |a Cunningham, Steve |e investigator |4 oth | |
| 700 | 1 | |a Beutels, Philippe |e investigator |4 oth | |
| 700 | 1 | |a Bont, Louis |e investigator |4 oth | |
| 700 | 1 | |a Wildenbeest, Joanne |e investigator |4 oth | |
| 700 | 1 | |a Pollard, Andrew |e investigator |4 oth | |
| 700 | 1 | |a Butler, Christopher |e investigator |4 oth | |
| 700 | 1 | |a Snape, Matthew |e investigator |4 oth | |
| 700 | 1 | |a Drysdale, Simon |e investigator |4 oth | |
| 700 | 1 | |a Lin, Gu-Lung |e investigator |4 oth | |
| 700 | 1 | |a O'Connor, Daniel |e investigator |4 oth | |
| 700 | 1 | |a Clutterbuck, Elizabeth |e investigator |4 oth | |
| 700 | 1 | |a Jefferies, Kimberley |e investigator |4 oth | |
| 700 | 1 | |a McGinley, Joseph |e investigator |4 oth | |
| 700 | 1 | |a Openshaw, Peter |e investigator |4 oth | |
| 700 | 1 | |a Thwaites, Ryan |e investigator |4 oth | |
| 700 | 1 | |a Wiseman, Dexter |e investigator |4 oth | |
| 700 | 1 | |a Martinon-Torres, Federico |e investigator |4 oth | |
| 700 | 1 | |a Gómez-Carballa, Alberto |e investigator |4 oth | |
| 700 | 1 | |a Heikkinen, Terho |e investigator |4 oth | |
| 700 | 1 | |a Meijer, Adam |e investigator |4 oth | |
| 700 | 1 | |a Fischer, Thea Kølsen |e investigator |4 oth | |
| 700 | 1 | |a van den Berge, Maarten |e investigator |4 oth | |
| 700 | 1 | |a Giaquinto, Carlo |e investigator |4 oth | |
| 700 | 1 | |a Abram, Michael |e investigator |4 oth | |
| 700 | 1 | |a Swanson, Kena |e investigator |4 oth | |
| 700 | 1 | |a Leach, Amanda |e investigator |4 oth | |
| 700 | 1 | |a Demont, Clarisse |e investigator |4 oth | |
| 700 | 1 | |a Gallichan, Scott |e investigator |4 oth | |
| 700 | 1 | |a Aerssens, Jeroen |e investigator |4 oth | |
| 700 | 1 | |a Öner, Deniz |e investigator |4 oth | |
| 700 | 1 | |a Rosen, Brian |e investigator |4 oth | |
| 700 | 1 | |a Molero, Eva |e investigator |4 oth | |
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