Genetic heterogeneity of canine bufaviruses
© 2020 Blackwell Verlag GmbH..
Canine bufavirus (CBuV) is a protoparvovirus, genetically related to human and non-human primate bufaviruses and distantly related to canine parvovirus type 2 (CPV-2). CBuV was initially identified from young dogs with respiratory signs but subsequent studies revealed that this virus is also a common component of the canine enteric virome. In this survey, by assessing archival and recent collections of dogs faecal samples, CBuV DNA was detected with a higher prevalence rate (8.8%) in animals with enteritis than in control animals (5.0%), although this difference was not statistically significant. The rate of co-infections with other enteric viruses in diarrhoeic dogs was high (84.6%), mostly in association with canine parvovirus CPV-2 (90.1%). The complete ORF2 gene was determined in five samples, and the nearly full-length genome was reconstructed for three strains, 62/2017/ITA, 9AS/2005/ITA and 35/2018/ITA. Upon sequence comparison, the viruses appeared highly conserved in the NS1 (97.2%-97.9% nt and 97.5%-98.1% aa identities). In the complete VP2 coding region, three strains were similar to the prototype viruses (99.7-99.8 nt and 99.6%-99.8% aa) whilst strains 9AS/2005/ITA and 35/2016/ITA were distantly related (87.6%-89.3% nt and 93.9%-95.1% aa identities). Interestingly, genetic diversification occurred downstream conserved regions such as the VP1/VP2 splicing signals and/or the G-rich motif in the N terminus of the VP2, suggesting a potential recombination nature. Upon phylogenetic analysis, the two divergent CBuV strains formed a distinct cluster/genotype.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2021 |
|---|---|
| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:68 |
|---|---|
| Enthalten in: |
Transboundary and emerging diseases - 68(2021), 2 vom: 03. März, Seite 802-812 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Di Martino, Barbara [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Canine bufavirus |
|---|
| Anmerkungen: |
Date Completed 30.06.2021 Date Revised 30.06.2021 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1111/tbed.13746 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM312647611 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM312647611 | ||
| 003 | DE-627 | ||
| 005 | 20231225144851.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2021 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1111/tbed.13746 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1042.xml |
| 035 | |a (DE-627)NLM312647611 | ||
| 035 | |a (NLM)32688446 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Di Martino, Barbara |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Genetic heterogeneity of canine bufaviruses |
| 264 | 1 | |c 2021 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 30.06.2021 | ||
| 500 | |a Date Revised 30.06.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2020 Blackwell Verlag GmbH. | ||
| 520 | |a Canine bufavirus (CBuV) is a protoparvovirus, genetically related to human and non-human primate bufaviruses and distantly related to canine parvovirus type 2 (CPV-2). CBuV was initially identified from young dogs with respiratory signs but subsequent studies revealed that this virus is also a common component of the canine enteric virome. In this survey, by assessing archival and recent collections of dogs faecal samples, CBuV DNA was detected with a higher prevalence rate (8.8%) in animals with enteritis than in control animals (5.0%), although this difference was not statistically significant. The rate of co-infections with other enteric viruses in diarrhoeic dogs was high (84.6%), mostly in association with canine parvovirus CPV-2 (90.1%). The complete ORF2 gene was determined in five samples, and the nearly full-length genome was reconstructed for three strains, 62/2017/ITA, 9AS/2005/ITA and 35/2018/ITA. Upon sequence comparison, the viruses appeared highly conserved in the NS1 (97.2%-97.9% nt and 97.5%-98.1% aa identities). In the complete VP2 coding region, three strains were similar to the prototype viruses (99.7-99.8 nt and 99.6%-99.8% aa) whilst strains 9AS/2005/ITA and 35/2016/ITA were distantly related (87.6%-89.3% nt and 93.9%-95.1% aa identities). Interestingly, genetic diversification occurred downstream conserved regions such as the VP1/VP2 splicing signals and/or the G-rich motif in the N terminus of the VP2, suggesting a potential recombination nature. Upon phylogenetic analysis, the two divergent CBuV strains formed a distinct cluster/genotype | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a canine bufavirus | |
| 650 | 4 | |a faecal samples | |
| 650 | 4 | |a genetic heterogeneity | |
| 650 | 4 | |a protoparvovirus | |
| 700 | 1 | |a Sarchese, Vittorio |e verfasserin |4 aut | |
| 700 | 1 | |a Di Profio, Federica |e verfasserin |4 aut | |
| 700 | 1 | |a Palombieri, Andrea |e verfasserin |4 aut | |
| 700 | 1 | |a Melegari, Irene |e verfasserin |4 aut | |
| 700 | 1 | |a Fruci, Paola |e verfasserin |4 aut | |
| 700 | 1 | |a Aste, Giovanni |e verfasserin |4 aut | |
| 700 | 1 | |a Bányai, Krisztián |e verfasserin |4 aut | |
| 700 | 1 | |a Fulvio, Marsilio |e verfasserin |4 aut | |
| 700 | 1 | |a Martella, Vito |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Transboundary and emerging diseases |d 2008 |g 68(2021), 2 vom: 03. März, Seite 802-812 |w (DE-627)NLM178784648 |x 1865-1682 |7 nnns |
| 773 | 1 | 8 | |g volume:68 |g year:2021 |g number:2 |g day:03 |g month:03 |g pages:802-812 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1111/tbed.13746 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 68 |j 2021 |e 2 |b 03 |c 03 |h 802-812 | ||