Predominant differentiation of rat fetal neural stem cells into functional oligodendrocytes in vitro
Copyright © 2020 Elsevier B.V. All rights reserved..
Oligodendrocytes form myelin in the CNS. A fast method to produce large quantity of oligodendrocytes that have the capacity of myelinating CNS neurons would be very useful for treating CNS injuries or demyelinating diseases, or for research purposes. We developed a simple standard protocol for predominant differentiation of rat fetal neural stem cells (NSCs) into oligodendrocytes. We adopted a new method to purify the oligodendrocytes and co-cultured the newly differentiated oligodendrocytes with hippocampal neurons to confirm their myelination capability. NSCs from embryonic day 14 (E14) were propagated at the presence of basic fibroblast growth factor and platelet derived growth factor alpha, and then differentiated in the medium containing triiodothyronine. Four extracellular matrix (ECM), poly-d-lysine (PDL), PDL-laminin, fibronectin, and matrigel, were examined for NSC differentiation. About 90 % of NSCs differentiated into oligodendrocytes on matrigel compared to 32 % on PDL or PDL-laminin, and 26 % on fibronectin after 3 weeks of differentiation, demonstrating the significant influence of ECM. Further, newly differentiated oligodendrocytes were co-cultured with hippocampal neurons from E18 rat embryos resulting in robust myelination of neurites at three weeks. In summary, we present a simplified and efficient method to predominantly generate oligodendrocytes from NSCs that is potentially very useful for CNS demyelination diseases.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2020 |
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Erschienen: |
2020 |
Enthalten in: |
Zur Gesamtaufnahme - volume:736 |
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Enthalten in: |
Neuroscience letters - 736(2020) vom: 25. Sept., Seite 135264 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Sharma, Krishna Deo [VerfasserIn] |
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Links: |
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Themen: |
Differentiation |
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Anmerkungen: |
Date Completed 13.05.2021 Date Revised 13.05.2021 published: Print-Electronic Citation Status MEDLINE |
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doi: |
10.1016/j.neulet.2020.135264 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM312642695 |
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520 | |a Oligodendrocytes form myelin in the CNS. A fast method to produce large quantity of oligodendrocytes that have the capacity of myelinating CNS neurons would be very useful for treating CNS injuries or demyelinating diseases, or for research purposes. We developed a simple standard protocol for predominant differentiation of rat fetal neural stem cells (NSCs) into oligodendrocytes. We adopted a new method to purify the oligodendrocytes and co-cultured the newly differentiated oligodendrocytes with hippocampal neurons to confirm their myelination capability. NSCs from embryonic day 14 (E14) were propagated at the presence of basic fibroblast growth factor and platelet derived growth factor alpha, and then differentiated in the medium containing triiodothyronine. Four extracellular matrix (ECM), poly-d-lysine (PDL), PDL-laminin, fibronectin, and matrigel, were examined for NSC differentiation. About 90 % of NSCs differentiated into oligodendrocytes on matrigel compared to 32 % on PDL or PDL-laminin, and 26 % on fibronectin after 3 weeks of differentiation, demonstrating the significant influence of ECM. Further, newly differentiated oligodendrocytes were co-cultured with hippocampal neurons from E18 rat embryos resulting in robust myelination of neurites at three weeks. In summary, we present a simplified and efficient method to predominantly generate oligodendrocytes from NSCs that is potentially very useful for CNS demyelination diseases | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Research Support, U.S. Gov't, Non-P.H.S. | |
650 | 4 | |a Differentiation | |
650 | 4 | |a Extracellular matrix | |
650 | 4 | |a Myelination | |
650 | 4 | |a Oligodendrocytes | |
650 | 4 | |a Rat fetal neural stem cells | |
700 | 1 | |a Pandanaboina, Sahitya Chetan |e verfasserin |4 aut | |
700 | 1 | |a Srivatsan, Malathi |e verfasserin |4 aut | |
700 | 1 | |a Xie, Jennifer Yanhua |e verfasserin |4 aut | |
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