Severely ill COVID-19 patients display augmented functional properties in SARS-CoV-2-reactive CD8 + T cells
The molecular properties of CD8 + T cells that respond to SARS-CoV-2 infection are not fully known. Here, we report on the single-cell transcriptomes of >80,000 virus-reactive CD8 + T cells from 39 COVID-19 patients and 10 healthy subjects. COVID-19 patients segregated into two groups based on whether the dominant CD8 + T cell response to SARS-CoV-2 was 'exhausted' or not. SARS-CoV-2-reactive cells in the exhausted subset were increased in frequency and displayed lesser cytotoxicity and inflammatory features in COVID-19 patients with mild compared to severe illness. In contrast, SARS-CoV-2-reactive cells in the non-exhausted subsets from patients with severe disease showed enrichment of transcripts linked to co-stimulation, pro-survival NF-κB signaling, and anti-apoptotic pathways, suggesting the generation of robust CD8 + T cell memory responses in patients with severe COVID-19 illness. CD8 + T cells reactive to influenza and respiratory syncytial virus from healthy subjects displayed polyfunctional features. Cells with such features were mostly absent in SARS-CoV-2 responsive cells from both COVID-19 patients and healthy controls non-exposed to SARS-CoV-2. Overall, our single-cell analysis revealed substantial diversity in the nature of CD8 + T cells responding to SARS-CoV-2.
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - year:2020 |
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| Enthalten in: |
bioRxiv : the preprint server for biology - (2020) vom: 10. Juli |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Kusnadi, Anthony [VerfasserIn] |
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Date Revised 29.03.2024 published: Electronic UpdateIn: Sci Immunol. 2021 Jan 21;6(55):. - PMID 33478949 Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1101/2020.07.09.194027 |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM312529686 |
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| 500 | |a UpdateIn: Sci Immunol. 2021 Jan 21;6(55):. - PMID 33478949 | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a The molecular properties of CD8 + T cells that respond to SARS-CoV-2 infection are not fully known. Here, we report on the single-cell transcriptomes of >80,000 virus-reactive CD8 + T cells from 39 COVID-19 patients and 10 healthy subjects. COVID-19 patients segregated into two groups based on whether the dominant CD8 + T cell response to SARS-CoV-2 was 'exhausted' or not. SARS-CoV-2-reactive cells in the exhausted subset were increased in frequency and displayed lesser cytotoxicity and inflammatory features in COVID-19 patients with mild compared to severe illness. In contrast, SARS-CoV-2-reactive cells in the non-exhausted subsets from patients with severe disease showed enrichment of transcripts linked to co-stimulation, pro-survival NF-κB signaling, and anti-apoptotic pathways, suggesting the generation of robust CD8 + T cell memory responses in patients with severe COVID-19 illness. CD8 + T cells reactive to influenza and respiratory syncytial virus from healthy subjects displayed polyfunctional features. Cells with such features were mostly absent in SARS-CoV-2 responsive cells from both COVID-19 patients and healthy controls non-exposed to SARS-CoV-2. Overall, our single-cell analysis revealed substantial diversity in the nature of CD8 + T cells responding to SARS-CoV-2 | ||
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| 700 | 1 | |a Fajardo, Vicente |e verfasserin |4 aut | |
| 700 | 1 | |a Chee, Serena J |e verfasserin |4 aut | |
| 700 | 1 | |a Meckiff, Benjamin J |e verfasserin |4 aut | |
| 700 | 1 | |a Simon, Hayley |e verfasserin |4 aut | |
| 700 | 1 | |a Pelosi, Emanuela |e verfasserin |4 aut | |
| 700 | 1 | |a Seumois, Grégory |e verfasserin |4 aut | |
| 700 | 1 | |a Ay, Ferhat |e verfasserin |4 aut | |
| 700 | 1 | |a Vijayanand, Pandurangan |e verfasserin |4 aut | |
| 700 | 1 | |a Ottensmeier, Christian H |e verfasserin |4 aut | |
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