Exosomal expression of RAB27A and its related lncRNA Lnc-RNA-RP11-510M2 in lung cancer
OBJECTIVES: Examine the diagnostic role of serum exosomal RAB27A mRNA in lung cancer and evaluate the relation of LncRNAs to lung cancer in association to RAB27A mRNA in Egyptian population.
METHODS: Exosomal RNA-based biomarkers RAB27A mRNA and Lnc-RNA-RP11-510M2.10 were selected based on bioinformatic methods, followed by RT-qPCR validation of their expression in serum of 20 patients with lung cancer, 10 patients with COPD and 10 healthy volunteers. we examined their expression in 10 bronchoalveolar lavage samples and assessed correlation with the serum levels.
RESULTS: There was an inverse relationship between expression of serum exosomal RAB27A mRNA and Lnc-RNA-RP11-510M2.10 (r = -0.62, p = .00). Both serum exosomal RAB27A mRNA and Lnc-RNA-RP11-510M2.10 showed a significant positive and negative association with lung cancer patients respectively in comparison to patients with COPD and healthy persons (p < .001).
CONCLUSION: RAB27A mRNA and Lnc-RNA-RP11-510M2.10 could be used as diagnostic and prognostic biomarker tools for lung cancer.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2022 |
|---|---|
| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:128 |
|---|---|
| Enthalten in: |
Archives of physiology and biochemistry - 128(2022), 6 vom: 02. Dez., Seite 1479-1485 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Mohamed Gamal El-Din, Ghada [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Biomarkers |
|---|
| Anmerkungen: |
Date Completed 24.10.2022 Date Revised 24.10.2022 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1080/13813455.2020.1778036 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM312338767 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM312338767 | ||
| 003 | DE-627 | ||
| 005 | 20231225144214.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2022 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1080/13813455.2020.1778036 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1041.xml |
| 035 | |a (DE-627)NLM312338767 | ||
| 035 | |a (NLM)32657170 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Mohamed Gamal El-Din, Ghada |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Exosomal expression of RAB27A and its related lncRNA Lnc-RNA-RP11-510M2 in lung cancer |
| 264 | 1 | |c 2022 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 24.10.2022 | ||
| 500 | |a Date Revised 24.10.2022 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a OBJECTIVES: Examine the diagnostic role of serum exosomal RAB27A mRNA in lung cancer and evaluate the relation of LncRNAs to lung cancer in association to RAB27A mRNA in Egyptian population | ||
| 520 | |a METHODS: Exosomal RNA-based biomarkers RAB27A mRNA and Lnc-RNA-RP11-510M2.10 were selected based on bioinformatic methods, followed by RT-qPCR validation of their expression in serum of 20 patients with lung cancer, 10 patients with COPD and 10 healthy volunteers. we examined their expression in 10 bronchoalveolar lavage samples and assessed correlation with the serum levels | ||
| 520 | |a RESULTS: There was an inverse relationship between expression of serum exosomal RAB27A mRNA and Lnc-RNA-RP11-510M2.10 (r = -0.62, p = .00). Both serum exosomal RAB27A mRNA and Lnc-RNA-RP11-510M2.10 showed a significant positive and negative association with lung cancer patients respectively in comparison to patients with COPD and healthy persons (p < .001) | ||
| 520 | |a CONCLUSION: RAB27A mRNA and Lnc-RNA-RP11-510M2.10 could be used as diagnostic and prognostic biomarker tools for lung cancer | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Lnc-RNA-RP11-510M2.10 | |
| 650 | 4 | |a RAB27A | |
| 650 | 4 | |a exosomal RNA extraction | |
| 650 | 4 | |a lung cancer | |
| 650 | 7 | |a RNA, Long Noncoding |2 NLM | |
| 650 | 7 | |a RNA, Messenger |2 NLM | |
| 650 | 7 | |a Biomarkers |2 NLM | |
| 650 | 7 | |a RAB27A protein, human |2 NLM | |
| 650 | 7 | |a EC 3.6.1.-. |2 NLM | |
| 650 | 7 | |a rab27 GTP-Binding Proteins |2 NLM | |
| 700 | 1 | |a Ibrahim, Fawzia Khalil |e verfasserin |4 aut | |
| 700 | 1 | |a Shehata, Hanan Hussein |e verfasserin |4 aut | |
| 700 | 1 | |a Osman, Nehad Mohammed |e verfasserin |4 aut | |
| 700 | 1 | |a Abdel-Rahman, Omar Mohammed |e verfasserin |4 aut | |
| 700 | 1 | |a Ali, Marwa |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Archives of physiology and biochemistry |d 1997 |g 128(2022), 6 vom: 02. Dez., Seite 1479-1485 |w (DE-627)NLM075599813 |x 1744-4160 |7 nnns |
| 773 | 1 | 8 | |g volume:128 |g year:2022 |g number:6 |g day:02 |g month:12 |g pages:1479-1485 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1080/13813455.2020.1778036 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 128 |j 2022 |e 6 |b 02 |c 12 |h 1479-1485 | ||