Recombinant SARS-CoV-2 spike S1-Fc fusion protein induced high levels of neutralizing responses in nonhuman primates
Copyright © 2020 The Authors. Published by Elsevier Ltd.. All rights reserved..
The COVID-19 outbreak has become a global pandemic responsible for over 2,000,000 confirmed cases and over 126,000 deaths worldwide. In this study, we examined the immunogenicity of CHO-expressed recombinant SARS-CoV-2 S1-Fc fusion protein in mice, rabbits, and monkeys as a potential candidate for a COVID-19 vaccine. We demonstrate that the S1-Fc fusion protein is extremely immunogenic, as evidenced by strong antibody titers observed by day 7. Strong virus neutralizing activity was observed on day 14 in rabbits immunized with the S1-Fc fusion protein using a pseudovirus neutralization assay. Most importantly, in <20 days and three injections of the S1-Fc fusion protein, two monkeys developed higher virus neutralizing titers than a recovered COVID-19 patient in a live SARS-CoV-2 infection assay. Our data strongly suggests that the CHO-expressed SARS-CoV-2 S1-Fc recombinant protein could be a strong candidate for vaccine development against COVID-19.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:38 |
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| Enthalten in: |
Vaccine - 38(2020), 35 vom: 31. Juli, Seite 5653-5658 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Ren, Wenlin [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 27.07.2020 Date Revised 28.04.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.vaccine.2020.06.066 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM312278616 |
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| 100 | 1 | |a Ren, Wenlin |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Recombinant SARS-CoV-2 spike S1-Fc fusion protein induced high levels of neutralizing responses in nonhuman primates |
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| 500 | |a Date Completed 27.07.2020 | ||
| 500 | |a Date Revised 28.04.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2020 The Authors. Published by Elsevier Ltd.. All rights reserved. | ||
| 520 | |a The COVID-19 outbreak has become a global pandemic responsible for over 2,000,000 confirmed cases and over 126,000 deaths worldwide. In this study, we examined the immunogenicity of CHO-expressed recombinant SARS-CoV-2 S1-Fc fusion protein in mice, rabbits, and monkeys as a potential candidate for a COVID-19 vaccine. We demonstrate that the S1-Fc fusion protein is extremely immunogenic, as evidenced by strong antibody titers observed by day 7. Strong virus neutralizing activity was observed on day 14 in rabbits immunized with the S1-Fc fusion protein using a pseudovirus neutralization assay. Most importantly, in <20 days and three injections of the S1-Fc fusion protein, two monkeys developed higher virus neutralizing titers than a recovered COVID-19 patient in a live SARS-CoV-2 infection assay. Our data strongly suggests that the CHO-expressed SARS-CoV-2 S1-Fc recombinant protein could be a strong candidate for vaccine development against COVID-19 | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a COVID-19 | |
| 650 | 4 | |a SARS-CoV-2 | |
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| 650 | 7 | |a Antibodies, Viral |2 NLM | |
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| 650 | 7 | |a Immunoglobulin M |2 NLM | |
| 650 | 7 | |a Spike Glycoprotein, Coronavirus |2 NLM | |
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| 700 | 1 | |a Sun, Hunter |e verfasserin |4 aut | |
| 700 | 1 | |a Gao, George F |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Jianxin |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Sean |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Rongqing |e verfasserin |4 aut | |
| 700 | 1 | |a Gao, Guang |e verfasserin |4 aut | |
| 700 | 1 | |a Hu, Yalin |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Gan |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Yuxin |e verfasserin |4 aut | |
| 700 | 1 | |a Jin, Xia |e verfasserin |4 aut | |
| 700 | 1 | |a Fang, Feng |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Jinggong |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Qi |e verfasserin |4 aut | |
| 700 | 1 | |a Gong, Sitao |e verfasserin |4 aut | |
| 700 | 1 | |a Gao, Wen |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Yufei |e verfasserin |4 aut | |
| 700 | 1 | |a Su, Junchi |e verfasserin |4 aut | |
| 700 | 1 | |a He, Ailiang |e verfasserin |4 aut | |
| 700 | 1 | |a Cheng, Xin |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Min |e verfasserin |4 aut | |
| 700 | 1 | |a Xia, Chenxi |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Maohua |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Le |e verfasserin |4 aut | |
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