C1 Inhibitor Administration Reduces Local Inflammation and Capillary Leakage, Without Affecting Long-term Wound Healing Parameters, in a Pig Burn Wound Model
Copyright© Bentham Science Publishers; For any queries, please email at epubbenthamscience.net..
BACKGROUND: Burns induce a boost in local and systemic complement levels as well as immune cell infiltration in the burn wound, which may negatively affect wound healing.
OBJECTIVE: In this study, the effects of long-term treatment with complement inhibitor C1 esterase inhibitor (C1inh) on post-burn inflammation and wound healing parameters were analyzed in time up to 60 days post-burn.
METHODS: Burned pigs were treated either with or without C1inh up to 15 days post-burn. Burn wound biopsies and blood were collected at different time points up to 60 days post-burn. Thereafter, complement in blood as well as complement and immune cells in the wound, capillary leakage, necrosis, reepithelialization and wound contraction were quantified.
RESULTS: No significant differences in complement C3 blood levels were observed at any time point between C1inh-treated and control pigs. In the wound, complement C4 levels were significantly lower in the C1inh group than in controls at day 3-6 and 21-30 post-burn. Similarly, C3 levels, neutrophil and macrophage infiltration in the wound were, although not statistically significant, reduced in C1inh-treated pigs at day 9-14 post-burn. No differences in lymphocyte infiltration in the wound were found between C1inh and control pigs. C1inh-treated pigs also showed reduced capillary leakage. Despite these effects, no significant differences in the long-term wound healing parameters necrosis, reepithelialization and wound contraction were observed between C1inh and control pigs.
CONCLUSION: In pigs, 15 days of C1inh treatment after burn, leads to a reduction in local inflammation and capillary leakage in the burn wound without affecting long-term wound healing parameters.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:20 |
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| Enthalten in: |
Anti-inflammatory & anti-allergy agents in medicinal chemistry - 20(2021), 2 vom: 02., Seite 150-160 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Korkmaz, Halil Ibrahim [VerfasserIn] |
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| Links: |
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| Themen: |
Burn wound |
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| Anmerkungen: |
Date Completed 23.11.2021 Date Revised 23.11.2021 published: Print Citation Status MEDLINE |
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| doi: |
10.2174/1871523019666200702101513 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM311925545 |
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| 100 | 1 | |a Korkmaz, Halil Ibrahim |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a C1 Inhibitor Administration Reduces Local Inflammation and Capillary Leakage, Without Affecting Long-term Wound Healing Parameters, in a Pig Burn Wound Model |
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| 500 | |a Date Completed 23.11.2021 | ||
| 500 | |a Date Revised 23.11.2021 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright© Bentham Science Publishers; For any queries, please email at epubbenthamscience.net. | ||
| 520 | |a BACKGROUND: Burns induce a boost in local and systemic complement levels as well as immune cell infiltration in the burn wound, which may negatively affect wound healing | ||
| 520 | |a OBJECTIVE: In this study, the effects of long-term treatment with complement inhibitor C1 esterase inhibitor (C1inh) on post-burn inflammation and wound healing parameters were analyzed in time up to 60 days post-burn | ||
| 520 | |a METHODS: Burned pigs were treated either with or without C1inh up to 15 days post-burn. Burn wound biopsies and blood were collected at different time points up to 60 days post-burn. Thereafter, complement in blood as well as complement and immune cells in the wound, capillary leakage, necrosis, reepithelialization and wound contraction were quantified | ||
| 520 | |a RESULTS: No significant differences in complement C3 blood levels were observed at any time point between C1inh-treated and control pigs. In the wound, complement C4 levels were significantly lower in the C1inh group than in controls at day 3-6 and 21-30 post-burn. Similarly, C3 levels, neutrophil and macrophage infiltration in the wound were, although not statistically significant, reduced in C1inh-treated pigs at day 9-14 post-burn. No differences in lymphocyte infiltration in the wound were found between C1inh and control pigs. C1inh-treated pigs also showed reduced capillary leakage. Despite these effects, no significant differences in the long-term wound healing parameters necrosis, reepithelialization and wound contraction were observed between C1inh and control pigs | ||
| 520 | |a CONCLUSION: In pigs, 15 days of C1inh treatment after burn, leads to a reduction in local inflammation and capillary leakage in the burn wound without affecting long-term wound healing parameters | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a C1 inhibitor | |
| 650 | 4 | |a burn wound | |
| 650 | 4 | |a capillary leakage | |
| 650 | 4 | |a complement | |
| 650 | 4 | |a inflammation | |
| 650 | 4 | |a pig burn wound model | |
| 650 | 4 | |a wound healing | |
| 650 | 7 | |a Complement C1 Inhibitor Protein |2 NLM | |
| 700 | 1 | |a Ulrich, Magda M W |e verfasserin |4 aut | |
| 700 | 1 | |a Wieringen, Wessel N Van |e verfasserin |4 aut | |
| 700 | 1 | |a Doǧan, Hatice |e verfasserin |4 aut | |
| 700 | 1 | |a Vlig, Marcel |e verfasserin |4 aut | |
| 700 | 1 | |a Emmens, Reindert W |e verfasserin |4 aut | |
| 700 | 1 | |a Meyer, Klaas W |e verfasserin |4 aut | |
| 700 | 1 | |a Sinnige, Paul |e verfasserin |4 aut | |
| 700 | 1 | |a Zeerleder, Sacha |e verfasserin |4 aut | |
| 700 | 1 | |a Wouters, Diana |e verfasserin |4 aut | |
| 700 | 1 | |a Ham, Marieke S Van |e verfasserin |4 aut | |
| 700 | 1 | |a Zuijlen, Paul P M Van |e verfasserin |4 aut | |
| 700 | 1 | |a Krijnen, Paul A J |e verfasserin |4 aut | |
| 700 | 1 | |a Niessen, Hans W M |e verfasserin |4 aut | |
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