Integration of Tumor Mutation Burden and PD-L1 Testing in Routine Laboratory Diagnostics in Non-Small Cell Lung Cancer

In recent years, Non-small cell lung cancer (NSCLC) has evolved into a prime example for precision oncology with multiple FDA-approved "precision" drugs. For the majority of NSCLC lacking targetable genetic alterations, immune checkpoint inhibition (ICI) has become standard of care in first-line treatment or beyond. PD-L1 tumor expression represents the only approved predictive biomarker for PD-L1/PD-1 checkpoint inhibition by therapeutic antibodies. Since PD-L1-negative or low-expressing tumors may also respond to ICI, additional factors are likely to contribute in addition to PD-L1 expression. Tumor mutation burden (TMB) has emerged as a potential candidate; however, it is the most complex biomarker so far and might represent a challenge for routine diagnostics. We therefore established a hybrid capture (HC) next-generation sequencing (NGS) assay that covers all oncogenic driver alterations as well as TMB and validated TMB values by correlation with the assay (F1CDx) used for the CheckMate 227 study. Results of the first consecutive 417 patients analyzed in a routine clinical setting are presented. Data show that fast reliable comprehensive diagnostics including TMB and targetable alterations are obtained with a short turn-around time. Thus, even complex biomarkers can easily be implemented in routine practice to optimize treatment decisions for advanced NSCLC.

Medienart:

E-Artikel

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

Zur Gesamtaufnahme - volume:12

Enthalten in:

Cancers - 12(2020), 6 vom: 24. Juni

Sprache:

Englisch

Beteiligte Personen:

Schatz, Stefanie [VerfasserIn]
Falk, Markus [VerfasserIn]
Jóri, Balázs [VerfasserIn]
Ramdani, Hayat O [VerfasserIn]
Schmidt, Stefanie [VerfasserIn]
Willing, Eva-Maria [VerfasserIn]
Menon, Roopika [VerfasserIn]
Groen, Harry J M [VerfasserIn]
Diehl, Linda [VerfasserIn]
Kröger, Matthias [VerfasserIn]
Wesseler, Claas [VerfasserIn]
Griesinger, Frank [VerfasserIn]
Hoffknecht, Petra [VerfasserIn]
Tiemann, Markus [VerfasserIn]
Heukamp, Lukas C [VerfasserIn]

Links:

Volltext

Themen:

Driver mutation
Immuno-oncology
Journal Article
Lung cancer
PD-L1
Routine diagnostics
Tumor mutational burden

Anmerkungen:

Date Revised 28.09.2020

published: Electronic

Citation Status PubMed-not-MEDLINE

doi:

10.3390/cancers12061685

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM311780288