Evaluation of rapid antigen test for detection of SARS-CoV-2 virus
Copyright © 2020 Elsevier B.V. All rights reserved..
BACKGROUND: The rapid diagnosis of Coronavirus Disease 2019 (COVID-19) patients is essential to reduce the disease spread. Rapid antigen detection (RAD) tests are available, however, there is scanty data on the performance of RAD tests.
OBJECTIVE: To evaluate the performance of the commercially available BIOCREDIT COVID-19 Ag test and compare it with RT-PCR for detecting Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) virus. Analytical sensitivity for the detection of SARS-CoV-2 virus was determined for the RAD test using viral culture and RT-PCR as reference methods. The RAD test was further evaluated using respiratory samples collected from confirmed COVID-19 patients. The results were compared with RT-PCR test.
RESULTS: The detection limits between RAD test, viral culture and RT-PCR varied hugely. RAD was 103 fold less sensitive than viral culture while RAD was 105 fold less sensitive than RT-PCR. The RAD test detected between 11.1 % and 45.7 % of RT-PCR-positive samples from COVID-19 patients.
CONCLUSIONS: This study demonstrated that the RAD test serves only as adjunct to RT-PCR test because of potential for false-negative results.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:129 |
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| Enthalten in: |
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology - 129(2020) vom: 24. Aug., Seite 104500 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Mak, Gannon Ck [VerfasserIn] |
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| Links: |
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| Themen: |
2019 novel coronavirus |
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| Anmerkungen: |
Date Completed 11.08.2020 Date Revised 10.01.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.jcv.2020.104500 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM311639496 |
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| 500 | |a Date Completed 11.08.2020 | ||
| 500 | |a Date Revised 10.01.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2020 Elsevier B.V. All rights reserved. | ||
| 520 | |a BACKGROUND: The rapid diagnosis of Coronavirus Disease 2019 (COVID-19) patients is essential to reduce the disease spread. Rapid antigen detection (RAD) tests are available, however, there is scanty data on the performance of RAD tests | ||
| 520 | |a OBJECTIVE: To evaluate the performance of the commercially available BIOCREDIT COVID-19 Ag test and compare it with RT-PCR for detecting Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) virus. Analytical sensitivity for the detection of SARS-CoV-2 virus was determined for the RAD test using viral culture and RT-PCR as reference methods. The RAD test was further evaluated using respiratory samples collected from confirmed COVID-19 patients. The results were compared with RT-PCR test | ||
| 520 | |a RESULTS: The detection limits between RAD test, viral culture and RT-PCR varied hugely. RAD was 103 fold less sensitive than viral culture while RAD was 105 fold less sensitive than RT-PCR. The RAD test detected between 11.1 % and 45.7 % of RT-PCR-positive samples from COVID-19 patients | ||
| 520 | |a CONCLUSIONS: This study demonstrated that the RAD test serves only as adjunct to RT-PCR test because of potential for false-negative results | ||
| 650 | 4 | |a Evaluation Study | |
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a 2019 novel coronavirus | |
| 650 | 4 | |a COVID-19 | |
| 650 | 4 | |a RT-PCR | |
| 650 | 4 | |a Rapid antigen detection | |
| 650 | 4 | |a SARS-CoV-2 | |
| 650 | 4 | |a Viral culture | |
| 650 | 7 | |a Antigens, Viral |2 NLM | |
| 700 | 1 | |a Cheng, Peter Kc |e verfasserin |4 aut | |
| 700 | 1 | |a Lau, Stephen Sy |e verfasserin |4 aut | |
| 700 | 1 | |a Wong, Kitty Ky |e verfasserin |4 aut | |
| 700 | 1 | |a Lau, C S |e verfasserin |4 aut | |
| 700 | 1 | |a Lam, Edman Tk |e verfasserin |4 aut | |
| 700 | 1 | |a Chan, Rickjason Cw |e verfasserin |4 aut | |
| 700 | 1 | |a Tsang, Dominic Nc |e verfasserin |4 aut | |
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