Minimal cobalt metabolism in the marine cyanobacterium Prochlorococcus
Despite very low concentrations of cobalt in marine waters, cyanobacteria in the genus Prochlorococcus retain the genetic machinery for the synthesis and use of cobalt-bearing cofactors (cobalamins) in their genomes. We explore cobalt metabolism in a Prochlorococcus isolate from the equatorial Pacific Ocean (strain MIT9215) through a series of growth experiments under iron- and cobalt-limiting conditions. Metal uptake rates, quantitative proteomic measurements of cobalamin-dependent enzymes, and theoretical calculations all indicate that Prochlorococcus MIT9215 can sustain growth with less than 50 cobalt atoms per cell, ∼100-fold lower than minimum iron requirements for these cells (∼5,100 atoms per cell). Quantitative descriptions of Prochlorococcus cobalt limitation are used to interpret the cobalt distribution in the equatorial Pacific Ocean, where surface concentrations are among the lowest measured globally but Prochlorococcus biomass is high. A low minimum cobalt quota ensures that other nutrients, notably iron, will be exhausted before cobalt can be fully depleted, helping to explain the persistence of cobalt-dependent metabolism in marine cyanobacteria.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:117 |
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| Enthalten in: |
Proceedings of the National Academy of Sciences of the United States of America - 117(2020), 27 vom: 07. Juli, Seite 15740-15747 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Hawco, Nicholas J [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 04.09.2020 Date Revised 17.03.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1073/pnas.2001393117 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM311551637 |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Despite very low concentrations of cobalt in marine waters, cyanobacteria in the genus Prochlorococcus retain the genetic machinery for the synthesis and use of cobalt-bearing cofactors (cobalamins) in their genomes. We explore cobalt metabolism in a Prochlorococcus isolate from the equatorial Pacific Ocean (strain MIT9215) through a series of growth experiments under iron- and cobalt-limiting conditions. Metal uptake rates, quantitative proteomic measurements of cobalamin-dependent enzymes, and theoretical calculations all indicate that Prochlorococcus MIT9215 can sustain growth with less than 50 cobalt atoms per cell, ∼100-fold lower than minimum iron requirements for these cells (∼5,100 atoms per cell). Quantitative descriptions of Prochlorococcus cobalt limitation are used to interpret the cobalt distribution in the equatorial Pacific Ocean, where surface concentrations are among the lowest measured globally but Prochlorococcus biomass is high. A low minimum cobalt quota ensures that other nutrients, notably iron, will be exhausted before cobalt can be fully depleted, helping to explain the persistence of cobalt-dependent metabolism in marine cyanobacteria | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
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| 700 | 1 | |a McIlvin, Matthew M |e verfasserin |4 aut | |
| 700 | 1 | |a Bundy, Randelle M |e verfasserin |4 aut | |
| 700 | 1 | |a Tagliabue, Alessandro |e verfasserin |4 aut | |
| 700 | 1 | |a Goepfert, Tyler J |e verfasserin |4 aut | |
| 700 | 1 | |a Moran, Dawn M |e verfasserin |4 aut | |
| 700 | 1 | |a Valentin-Alvarado, Luis |e verfasserin |4 aut | |
| 700 | 1 | |a DiTullio, Giacomo R |e verfasserin |4 aut | |
| 700 | 1 | |a Saito, Mak A |e verfasserin |4 aut | |
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