NONO Is a Negative Regulator of SOX2 Promoter
Copyright© 2020, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved..
BACKGROUND/AIM: Sex determining region Y (SRY)-box 2 (SOX2) is a transcription factor essential for the maintenance of proliferation and self-renewal of cancer stem cells and is associated with breast cancer initiation. Regulation of cancer stem cell plasticity by SOX2 requires both positive and negative SOX2 transcription factors, but the negative regulator is still largely unknown.
MATERIALS AND METHODS: SOX2 promoter-binding proteins were identified by liquid chromatography-mass spectrometry/mass spectrometry, luciferase assay, and chromatin immunoprecipitation. The effects of one such transcription factor on SOX2 expression was investigated by knockdown and overexpression experiments.
RESULTS: Non-POU domain-containing octamer-binding protein (NONO) (also known as 54-kDa nuclear RNA-binding protein, P54NRB) was identified as a SOX2 promoter-binding protein and a negative regulator of SOX2 expression. Its activity was controlled by its coiled-coil domain and the C-terminal domain.
CONCLUSION: These results suggest that NONO acts as a key regulator of SOX2 transcription through the repression of SOX2 promoter activity in breast cancer cells.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:17 |
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| Enthalten in: |
Cancer genomics & proteomics - 17(2020), 4 vom: 23. Juli, Seite 359-367 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Liang, Shanshan [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 11.02.2021 Date Revised 11.02.2021 published: Print Citation Status MEDLINE |
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| doi: |
10.21873/cgp.20195 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM311550541 |
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| 245 | 1 | 0 | |a NONO Is a Negative Regulator of SOX2 Promoter |
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| 500 | |a Date Revised 11.02.2021 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright© 2020, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved. | ||
| 520 | |a BACKGROUND/AIM: Sex determining region Y (SRY)-box 2 (SOX2) is a transcription factor essential for the maintenance of proliferation and self-renewal of cancer stem cells and is associated with breast cancer initiation. Regulation of cancer stem cell plasticity by SOX2 requires both positive and negative SOX2 transcription factors, but the negative regulator is still largely unknown | ||
| 520 | |a MATERIALS AND METHODS: SOX2 promoter-binding proteins were identified by liquid chromatography-mass spectrometry/mass spectrometry, luciferase assay, and chromatin immunoprecipitation. The effects of one such transcription factor on SOX2 expression was investigated by knockdown and overexpression experiments | ||
| 520 | |a RESULTS: Non-POU domain-containing octamer-binding protein (NONO) (also known as 54-kDa nuclear RNA-binding protein, P54NRB) was identified as a SOX2 promoter-binding protein and a negative regulator of SOX2 expression. Its activity was controlled by its coiled-coil domain and the C-terminal domain | ||
| 520 | |a CONCLUSION: These results suggest that NONO acts as a key regulator of SOX2 transcription through the repression of SOX2 promoter activity in breast cancer cells | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a SOX2 | |
| 650 | 4 | |a breast cancer | |
| 650 | 4 | |a cancer stem cell | |
| 650 | 4 | |a negative regulator | |
| 650 | 4 | |a transcription factor | |
| 650 | 7 | |a Biomarkers, Tumor |2 NLM | |
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| 650 | 7 | |a SOX2 protein, human |2 NLM | |
| 650 | 7 | |a SOXB1 Transcription Factors |2 NLM | |
| 700 | 1 | |a Takahashi, Hidehisa |e verfasserin |4 aut | |
| 700 | 1 | |a Hirose, Tetsuro |e verfasserin |4 aut | |
| 700 | 1 | |a Kuramitsu, Yasuhiro |e verfasserin |4 aut | |
| 700 | 1 | |a Hatakeyama, Shigetsugu |e verfasserin |4 aut | |
| 700 | 1 | |a Yoshiyama, Hironori |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Ruoyu |e verfasserin |4 aut | |
| 700 | 1 | |a Hamada, Jun-Ichi |e verfasserin |4 aut | |
| 700 | 1 | |a Iizasa, Hisashi |e verfasserin |4 aut | |
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