Hypercoagulability (thrombin generation) in patients with cirrhosis is detected with ST-Genesia
© 2020 International Society on Thrombosis and Haemostasis..
BACKGROUND: Thrombin generation assays (TGAs) performed with calibrated automated thrombography (CAT) in the presence of thrombomodulin (TM) indicate plasma hypercoagulability in cirrhosis.
OBJECTIVE: To evaluate, in the presence of TM, the new ST-Genesia automated device developed for improving TGA vs the previously used CAT method, with plasma samples of patients with cirrhosis.
PATIENTS/METHODS: Platelet-poor plasma samples were prepared from citrated blood samples of 52 healthy controls and 85 patients with cirrhosis (severity evaluated using the Child-Pugh score [CP]). TGAs were performed using CAT with PPP-Reagent and ST-Genesia with the STG-ThromboScreen reagent, in the presence of TM. Endogenous thrombin potential (ETP) was chosen as the main parameter.
RESULTS: Whatever the method, ETP values were higher in patients than in healthy controls. All patients identified as hypercoagulable with ST-Genesia and STG-ThromboScreen were found hypercoagulable with CAT and PPP-Reagent. Conversely, eight and ten patients in the CP-A and CP-B classes respectively were identified as hypercoagulable only with CAT. The use of ST-Genesia with the STG-ThromboScreen reagent with TM led to a bias, with higher ETP values for healthy controls and lower for patients compared with CAT. Crossover analysis (CAT with the STG-ThromboScreen reagent) evidenced a substantial effect of the STG-ThromboScreen reagent; the analyzer (including calibration and data analysis) plays a lesser role.
CONCLUSION: ST-Genesia evidences hypercoagulability in patients with cirrhosis when TG is studied in the presence of TM, but the results are not interchangeable with those obtained with CAT.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:18 |
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| Enthalten in: |
Journal of thrombosis and haemostasis : JTH - 18(2020), 9 vom: 19. Sept., Seite 2177-2190 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Talon, Laurie [VerfasserIn] |
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| Links: |
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| Themen: |
Blood coagulation |
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| Anmerkungen: |
Date Completed 19.05.2021 Date Revised 29.08.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1111/jth.14963 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM311370322 |
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| 245 | 1 | 0 | |a Hypercoagulability (thrombin generation) in patients with cirrhosis is detected with ST-Genesia |
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| 500 | |a Date Revised 29.08.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2020 International Society on Thrombosis and Haemostasis. | ||
| 520 | |a BACKGROUND: Thrombin generation assays (TGAs) performed with calibrated automated thrombography (CAT) in the presence of thrombomodulin (TM) indicate plasma hypercoagulability in cirrhosis | ||
| 520 | |a OBJECTIVE: To evaluate, in the presence of TM, the new ST-Genesia automated device developed for improving TGA vs the previously used CAT method, with plasma samples of patients with cirrhosis | ||
| 520 | |a PATIENTS/METHODS: Platelet-poor plasma samples were prepared from citrated blood samples of 52 healthy controls and 85 patients with cirrhosis (severity evaluated using the Child-Pugh score [CP]). TGAs were performed using CAT with PPP-Reagent and ST-Genesia with the STG-ThromboScreen reagent, in the presence of TM. Endogenous thrombin potential (ETP) was chosen as the main parameter | ||
| 520 | |a RESULTS: Whatever the method, ETP values were higher in patients than in healthy controls. All patients identified as hypercoagulable with ST-Genesia and STG-ThromboScreen were found hypercoagulable with CAT and PPP-Reagent. Conversely, eight and ten patients in the CP-A and CP-B classes respectively were identified as hypercoagulable only with CAT. The use of ST-Genesia with the STG-ThromboScreen reagent with TM led to a bias, with higher ETP values for healthy controls and lower for patients compared with CAT. Crossover analysis (CAT with the STG-ThromboScreen reagent) evidenced a substantial effect of the STG-ThromboScreen reagent; the analyzer (including calibration and data analysis) plays a lesser role | ||
| 520 | |a CONCLUSION: ST-Genesia evidences hypercoagulability in patients with cirrhosis when TG is studied in the presence of TM, but the results are not interchangeable with those obtained with CAT | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a blood coagulation | |
| 650 | 4 | |a blood coagulation test | |
| 650 | 4 | |a clinical laboratory techniques | |
| 650 | 4 | |a liver cirrhosis | |
| 650 | 4 | |a thrombomodulin | |
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| 700 | 1 | |a Lecompte, Thomas |e verfasserin |4 aut | |
| 700 | 1 | |a Pereira, Bruno |e verfasserin |4 aut | |
| 700 | 1 | |a Massoulié, Sylvie |e verfasserin |4 aut | |
| 700 | 1 | |a Abergel, Armand |e verfasserin |4 aut | |
| 700 | 1 | |a Lebreton, Aurélien |e verfasserin |4 aut | |
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