New PAR1 Agonist Peptide Demonstrates Protective Action in a Mouse Model of Photothrombosis-Induced Brain Ischemia
Copyright © 2020 Galkov, Kiseleva, Gulyaev, Sidorova and Gorbacheva..
Protease-activated receptors (PARs) are involved not only in hemostasis but also in the development of ischemic brain injury. In the present work, we examined in vivo effects of a new peptide (AP9) composing Asn47-Phen55 of PAR1 "tethered ligand" generated by activated protein C. We chose a mouse model of photothrombosis (PT)-induced ischemia to assess AP9 effects in vivo. To reveal the molecular mechanism of AP9 action, mice lacking β-arrestin-2 were used. AP9 was injected intravenously once 10 min before PT at doses of 0.2, 2, or 20 mg/kg, or twice, that is, 10 min before and 1 h after PT at a dose of 20 mg/kg. Lesion volume was measured by magnetic resonance imaging and staining of brain sections with tetrazolium salt. Neurologic deficit was estimated using the cylinder and the grid-walk tests. Blood-brain barrier (BBB) disruption was assessed by Evans blue dye extraction. Eosin-hematoxylin staining and immunohistochemical staining were applied to evaluate the number of undamaged neurons and activated glial cells in the penumbra. A single administration of AP9 (20 mg/kg), as well as its two injections (20 mg/kg), decreased brain lesion volume. A double administration of AP9 also reduced BBB disruption and neurological deficit in mice. We did not observe the protective effect of AP9 in mice lacking β-arrestin-2 after PT. Thus, we demonstrated for the first time protective properties of a PAR1 agonist peptide, AP9, in vivo. β-Arrestin-2 was required for the protective action of AP9 in PT-induced brain ischemia.
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2020 |
---|---|
Erschienen: |
2020 |
Enthalten in: |
Zur Gesamtaufnahme - volume:14 |
---|---|
Enthalten in: |
Frontiers in neuroscience - 14(2020) vom: 01., Seite 335 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Galkov, Maksim [VerfasserIn] |
---|
Links: |
---|
Themen: |
β-arrestin-2 |
---|
Anmerkungen: |
Date Revised 28.09.2020 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
---|
doi: |
10.3389/fnins.2020.00335 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
NLM311261477 |
---|
LEADER | 01000naa a22002652 4500 | ||
---|---|---|---|
001 | NLM311261477 | ||
003 | DE-627 | ||
005 | 20231225141857.0 | ||
007 | cr uuu---uuuuu | ||
008 | 231225s2020 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.3389/fnins.2020.00335 |2 doi | |
028 | 5 | 2 | |a pubmed24n1037.xml |
035 | |a (DE-627)NLM311261477 | ||
035 | |a (NLM)32547356 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Galkov, Maksim |e verfasserin |4 aut | |
245 | 1 | 0 | |a New PAR1 Agonist Peptide Demonstrates Protective Action in a Mouse Model of Photothrombosis-Induced Brain Ischemia |
264 | 1 | |c 2020 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Revised 28.09.2020 | ||
500 | |a published: Electronic-eCollection | ||
500 | |a Citation Status PubMed-not-MEDLINE | ||
520 | |a Copyright © 2020 Galkov, Kiseleva, Gulyaev, Sidorova and Gorbacheva. | ||
520 | |a Protease-activated receptors (PARs) are involved not only in hemostasis but also in the development of ischemic brain injury. In the present work, we examined in vivo effects of a new peptide (AP9) composing Asn47-Phen55 of PAR1 "tethered ligand" generated by activated protein C. We chose a mouse model of photothrombosis (PT)-induced ischemia to assess AP9 effects in vivo. To reveal the molecular mechanism of AP9 action, mice lacking β-arrestin-2 were used. AP9 was injected intravenously once 10 min before PT at doses of 0.2, 2, or 20 mg/kg, or twice, that is, 10 min before and 1 h after PT at a dose of 20 mg/kg. Lesion volume was measured by magnetic resonance imaging and staining of brain sections with tetrazolium salt. Neurologic deficit was estimated using the cylinder and the grid-walk tests. Blood-brain barrier (BBB) disruption was assessed by Evans blue dye extraction. Eosin-hematoxylin staining and immunohistochemical staining were applied to evaluate the number of undamaged neurons and activated glial cells in the penumbra. A single administration of AP9 (20 mg/kg), as well as its two injections (20 mg/kg), decreased brain lesion volume. A double administration of AP9 also reduced BBB disruption and neurological deficit in mice. We did not observe the protective effect of AP9 in mice lacking β-arrestin-2 after PT. Thus, we demonstrated for the first time protective properties of a PAR1 agonist peptide, AP9, in vivo. β-Arrestin-2 was required for the protective action of AP9 in PT-induced brain ischemia | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a PAR1 agonist peptide | |
650 | 4 | |a photothrombosis-induced focal ischemia | |
650 | 4 | |a protease-activated receptor 1 (PAR1) | |
650 | 4 | |a protein C | |
650 | 4 | |a β-arrestin-2 | |
700 | 1 | |a Kiseleva, Ekaterina |e verfasserin |4 aut | |
700 | 1 | |a Gulyaev, Mikhail |e verfasserin |4 aut | |
700 | 1 | |a Sidorova, Maria |e verfasserin |4 aut | |
700 | 1 | |a Gorbacheva, Liubov |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Frontiers in neuroscience |d 2007 |g 14(2020) vom: 01., Seite 335 |w (DE-627)NLM184194563 |x 1662-4548 |7 nnns |
773 | 1 | 8 | |g volume:14 |g year:2020 |g day:01 |g pages:335 |
856 | 4 | 0 | |u http://dx.doi.org/10.3389/fnins.2020.00335 |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 14 |j 2020 |b 01 |h 335 |