False-positive staining of thyroglobulin distinguished from mixed medullary and follicular thyroid carcinoma by duplex in situ hybridization
Medullary thyroid carcinoma (MTC) may mimic mixed medullary and follicular thyroid carcinoma (MMFTC). MTC originates from para-follicular cells, while MMFTC is an uncommon tumor characterized by coexistence of follicular and para-follicular cell-derived tumor populations. A 35-year-old woman was diagnosed with MTC but showed a hot nodule in thyroid scintigraphy. The tumor included diffusely-spread follicular lesions within it, which were immunostained with thyroglobulin and calcitonin. Immunofluorescence showed the presence of several tumor cells that were double-stained with thyroglobulin and calcitonin. To clarify whether or not the tumor was MMFTC, we used duplex in situ hybridization (ISH). Thyroglobulin and calcitonin-related polypeptide alpha mRNA were not expressed together in a single cell, so we suspected false-positive staining of tumor cells with thyroglobulin. To make comparisons with other follicular lesions in MTC, we searched our hospital database. Five cases within a ten-year period had been pathologically diagnosed as MTC. All had follicular lesions in the tumor, but unlike the other case, they were peripherally localized. Dual differentiation into follicular or para-follicular tumor cells was not indicated by either immunofluorescence or duplex ISH. Compared with the case suspected to be MMFTC, there was only mild invasion of tumor cells into the follicular epithelium. The extent of follicular lesions and invasiveness of tumor cells may be associated with pseudo-staining of thyroglobulin in MTC. Duplex ISH can distinguish MTC that are stained with thyroglobulin from MMFTC.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2020 |
|---|---|
| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:67 |
|---|---|
| Enthalten in: |
Endocrine journal - 67(2020), 10 vom: 28. Okt., Seite 1007-1017 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Takeshima, Ken [VerfasserIn] |
|---|
| Links: |
|---|
| Anmerkungen: |
Date Completed 07.09.2021 Date Revised 07.09.2021 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1507/endocrj.EJ20-0169 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM311021891 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM311021891 | ||
| 003 | DE-627 | ||
| 005 | 20231225141346.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2020 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1507/endocrj.EJ20-0169 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1036.xml |
| 035 | |a (DE-627)NLM311021891 | ||
| 035 | |a (NLM)32522910 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Takeshima, Ken |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a False-positive staining of thyroglobulin distinguished from mixed medullary and follicular thyroid carcinoma by duplex in situ hybridization |
| 264 | 1 | |c 2020 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 07.09.2021 | ||
| 500 | |a Date Revised 07.09.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Medullary thyroid carcinoma (MTC) may mimic mixed medullary and follicular thyroid carcinoma (MMFTC). MTC originates from para-follicular cells, while MMFTC is an uncommon tumor characterized by coexistence of follicular and para-follicular cell-derived tumor populations. A 35-year-old woman was diagnosed with MTC but showed a hot nodule in thyroid scintigraphy. The tumor included diffusely-spread follicular lesions within it, which were immunostained with thyroglobulin and calcitonin. Immunofluorescence showed the presence of several tumor cells that were double-stained with thyroglobulin and calcitonin. To clarify whether or not the tumor was MMFTC, we used duplex in situ hybridization (ISH). Thyroglobulin and calcitonin-related polypeptide alpha mRNA were not expressed together in a single cell, so we suspected false-positive staining of tumor cells with thyroglobulin. To make comparisons with other follicular lesions in MTC, we searched our hospital database. Five cases within a ten-year period had been pathologically diagnosed as MTC. All had follicular lesions in the tumor, but unlike the other case, they were peripherally localized. Dual differentiation into follicular or para-follicular tumor cells was not indicated by either immunofluorescence or duplex ISH. Compared with the case suspected to be MMFTC, there was only mild invasion of tumor cells into the follicular epithelium. The extent of follicular lesions and invasiveness of tumor cells may be associated with pseudo-staining of thyroglobulin in MTC. Duplex ISH can distinguish MTC that are stained with thyroglobulin from MMFTC | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Calcitonin | |
| 650 | 4 | |a Follicular lesion | |
| 650 | 4 | |a In situ hybridization | |
| 650 | 4 | |a Medullary thyroid carcinoma | |
| 650 | 4 | |a Mixed medullary and follicular thyroid carcinoma | |
| 650 | 7 | |a Procalcitonin |2 NLM | |
| 650 | 7 | |a RNA, Messenger |2 NLM | |
| 650 | 7 | |a Calcitonin |2 NLM | |
| 650 | 7 | |a 9007-12-9 |2 NLM | |
| 650 | 7 | |a Thyroglobulin |2 NLM | |
| 650 | 7 | |a 9010-34-8 |2 NLM | |
| 700 | 1 | |a Ariyasu, Hiroyuki |e verfasserin |4 aut | |
| 700 | 1 | |a Uraki, Shinsuke |e verfasserin |4 aut | |
| 700 | 1 | |a Morita, Shuhei |e verfasserin |4 aut | |
| 700 | 1 | |a Furukawa, Yasushi |e verfasserin |4 aut | |
| 700 | 1 | |a Inaba, Hidefumi |e verfasserin |4 aut | |
| 700 | 1 | |a Iwakura, Hiroshi |e verfasserin |4 aut | |
| 700 | 1 | |a Doi, Asako |e verfasserin |4 aut | |
| 700 | 1 | |a Warigaya, Kenji |e verfasserin |4 aut | |
| 700 | 1 | |a Murata, Shin-Ichi |e verfasserin |4 aut | |
| 700 | 1 | |a Enomoto, Keisuke |e verfasserin |4 aut | |
| 700 | 1 | |a Hotomi, Muneki |e verfasserin |4 aut | |
| 700 | 1 | |a Akamizu, Takashi |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Endocrine journal |d 1993 |g 67(2020), 10 vom: 28. Okt., Seite 1007-1017 |w (DE-627)NLM074989081 |x 1348-4540 |7 nnns |
| 773 | 1 | 8 | |g volume:67 |g year:2020 |g number:10 |g day:28 |g month:10 |g pages:1007-1017 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1507/endocrj.EJ20-0169 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 67 |j 2020 |e 10 |b 28 |c 10 |h 1007-1017 | ||