COVID-19 target : A specific target for novel coronavirus detection
© 2020 Elsevier Inc. All rights reserved..
An ongoing outbreak of pneumonia associated with a novel coronavirus has been reported worldwide and become a global health problem; hence, the diagnosis and differentiation of this virus from other types of coronavirus is essential to control of the disease. To this end, the analysis of genomics data plays a vital role in introducing a stronger target and consequently provides better results in laboratory examinations. The modified comparative genomics approach helps us to find novel specific targets by comparing two or more sequences on the nucleotide collection database. We, for the first time, detected ORF8 gene as a potential target for the detection of the novel coronavirus. Unlike previous reported genes (RdRP, E and N genes), ORF8 is entirely specific to the novel coronavirus (COVID-19) and has no cross-reactivity with other kinds of coronavirus. Accordingly, ORF8 gene can be used as an additional confirmatory assay.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:20 |
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| Enthalten in: |
Gene reports - 20(2020) vom: 27. Sept., Seite 100740 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Kakhki, Reza Kamali [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Revised 08.11.2022 published: Print-Electronic Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1016/j.genrep.2020.100740 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM310894948 |
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| 520 | |a © 2020 Elsevier Inc. All rights reserved. | ||
| 520 | |a An ongoing outbreak of pneumonia associated with a novel coronavirus has been reported worldwide and become a global health problem; hence, the diagnosis and differentiation of this virus from other types of coronavirus is essential to control of the disease. To this end, the analysis of genomics data plays a vital role in introducing a stronger target and consequently provides better results in laboratory examinations. The modified comparative genomics approach helps us to find novel specific targets by comparing two or more sequences on the nucleotide collection database. We, for the first time, detected ORF8 gene as a potential target for the detection of the novel coronavirus. Unlike previous reported genes (RdRP, E and N genes), ORF8 is entirely specific to the novel coronavirus (COVID-19) and has no cross-reactivity with other kinds of coronavirus. Accordingly, ORF8 gene can be used as an additional confirmatory assay | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a BLAST, basic local alignment search tool | |
| 650 | 4 | |a COVID-19 | |
| 650 | 4 | |a COVID-19, coronavirus disease 2019 | |
| 650 | 4 | |a Coronavirus | |
| 650 | 4 | |a Diagnosis | |
| 650 | 4 | |a MERS-CoV, Middle East respiratory syndrome coronavirus | |
| 650 | 4 | |a ORF-8 | |
| 650 | 4 | |a ORF-8, open reading frame 8 | |
| 650 | 4 | |a RT-PCR, reverse transcription polymerase chain reaction | |
| 650 | 4 | |a SARS-CoV, severe acute respiratory syndrome coronavirus | |
| 700 | 1 | |a Kakhki, Mohammad Kamali |e verfasserin |4 aut | |
| 700 | 1 | |a Neshani, Alireza |e verfasserin |4 aut | |
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