Identification of cross-reactive human single-chain variable fragments against phospholipases A2 from Lachesis muta and Bothrops spp venoms
Copyright © 2020 Elsevier Ltd. All rights reserved..
Bushmasters (Lachesis spp) and lancehead vipers (Bothrops spp) are two of the most dangerous snakes found in Latin America. Victims of envenoming by these snakes require urgent administration of antivenom. Here, we report the identification of a small set of broadly neutralizing human monoclonal single-chain variable fragment (scFv) antibodies targeting key phospholipases A2 from Lachesis and Bothrops spp using phage display technology and demonstrate their in vitro efficacy using a hemolysis assay.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2020 |
|---|---|
| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:184 |
|---|---|
| Enthalten in: |
Toxicon : official journal of the International Society on Toxinology - 184(2020) vom: 15. Sept., Seite 116-121 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Campos, Lucas B [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Antivenins |
|---|
| Anmerkungen: |
Date Completed 26.08.2020 Date Revised 26.08.2020 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.toxicon.2020.05.027 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM310851637 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM310851637 | ||
| 003 | DE-627 | ||
| 005 | 20231225141005.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2020 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.toxicon.2020.05.027 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1036.xml |
| 035 | |a (DE-627)NLM310851637 | ||
| 035 | |a (NLM)32505638 | ||
| 035 | |a (PII)S0041-0101(20)30264-6 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Campos, Lucas B |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Identification of cross-reactive human single-chain variable fragments against phospholipases A2 from Lachesis muta and Bothrops spp venoms |
| 264 | 1 | |c 2020 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 26.08.2020 | ||
| 500 | |a Date Revised 26.08.2020 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2020 Elsevier Ltd. All rights reserved. | ||
| 520 | |a Bushmasters (Lachesis spp) and lancehead vipers (Bothrops spp) are two of the most dangerous snakes found in Latin America. Victims of envenoming by these snakes require urgent administration of antivenom. Here, we report the identification of a small set of broadly neutralizing human monoclonal single-chain variable fragment (scFv) antibodies targeting key phospholipases A2 from Lachesis and Bothrops spp using phage display technology and demonstrate their in vitro efficacy using a hemolysis assay | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Antivenom | |
| 650 | 4 | |a Bothrops spp | |
| 650 | 4 | |a Cross-reactive antibodies | |
| 650 | 4 | |a Lachesis muta | |
| 650 | 4 | |a Monoclonal antibody | |
| 650 | 4 | |a Phage display | |
| 650 | 7 | |a Antivenins |2 NLM | |
| 650 | 7 | |a Crotalid Venoms |2 NLM | |
| 650 | 7 | |a Single-Chain Antibodies |2 NLM | |
| 700 | 1 | |a Pucca, Manuela B |e verfasserin |4 aut | |
| 700 | 1 | |a Silva, Luciano C |e verfasserin |4 aut | |
| 700 | 1 | |a Pessenda, Gabriela |e verfasserin |4 aut | |
| 700 | 1 | |a Filardi, Bruno A |e verfasserin |4 aut | |
| 700 | 1 | |a Cerni, Felipe A |e verfasserin |4 aut | |
| 700 | 1 | |a Oliveira, Isadora S |e verfasserin |4 aut | |
| 700 | 1 | |a Laustsen, Andreas H |e verfasserin |4 aut | |
| 700 | 1 | |a Arantes, Eliane C |e verfasserin |4 aut | |
| 700 | 1 | |a Barbosa, José E |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Toxicon : official journal of the International Society on Toxinology |d 1964 |g 184(2020) vom: 15. Sept., Seite 116-121 |w (DE-627)NLM00002371X |x 1879-3150 |7 nnns |
| 773 | 1 | 8 | |g volume:184 |g year:2020 |g day:15 |g month:09 |g pages:116-121 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.toxicon.2020.05.027 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 184 |j 2020 |b 15 |c 09 |h 116-121 | ||