From Electronic Sequence to Purified Protein Using Automated Gene Synthesis and In Vitro Transcription/Translation
De novo gene synthesis is the state-of-the-art method used to obtain genetic material adapted to the requirements of the host organism and a cornerstone for modern synthetic biology. Yet, little progress has been made regarding downstream processes of protein production from synthetic genetic material. The production of recombinant proteins traditionally requires extensive preparatory work including gene amplification, cloning, sequencing, transformation or transfection of the expression host, cultivation of living cells, and purification of the overexpressed protein. In this work we describe a fast and automated workflow for cell-free production of proteins starting from an electronic protein sequence or accession number. PRESTO (protein expression starting from oligonucleotides) seamlessly combines a tailored in silico sequence optimization with the assembly of short oligonucleotides into synthetic linear DNA expression cassettes, mammalian in vitro transcription/translation, and protein purification thereof. Integrated on a small liquid handling system it provides a hands-free high throughput source for functional synthetic proteins within 1 day.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:9 |
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| Enthalten in: |
ACS synthetic biology - 9(2020), 7 vom: 17. Juli, Seite 1714-1724 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Chiocchini, Claudia [VerfasserIn] |
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| Links: |
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| Themen: |
11096-26-7 |
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| Anmerkungen: |
Date Completed 19.07.2021 Date Revised 19.07.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1021/acssynbio.0c00060 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM310818931 |
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| 520 | |a De novo gene synthesis is the state-of-the-art method used to obtain genetic material adapted to the requirements of the host organism and a cornerstone for modern synthetic biology. Yet, little progress has been made regarding downstream processes of protein production from synthetic genetic material. The production of recombinant proteins traditionally requires extensive preparatory work including gene amplification, cloning, sequencing, transformation or transfection of the expression host, cultivation of living cells, and purification of the overexpressed protein. In this work we describe a fast and automated workflow for cell-free production of proteins starting from an electronic protein sequence or accession number. PRESTO (protein expression starting from oligonucleotides) seamlessly combines a tailored in silico sequence optimization with the assembly of short oligonucleotides into synthetic linear DNA expression cassettes, mammalian in vitro transcription/translation, and protein purification thereof. Integrated on a small liquid handling system it provides a hands-free high throughput source for functional synthetic proteins within 1 day | ||
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| 700 | 1 | |a Vattem, Krishna |e verfasserin |4 aut | |
| 700 | 1 | |a Liss, Michael |e verfasserin |4 aut | |
| 700 | 1 | |a Ludewig, Lisa |e verfasserin |4 aut | |
| 700 | 1 | |a Reusch, Tobias |e verfasserin |4 aut | |
| 700 | 1 | |a Rastogi, Ischwaku |e verfasserin |4 aut | |
| 700 | 1 | |a Webb, Brian |e verfasserin |4 aut | |
| 700 | 1 | |a Trefzer, Axel |e verfasserin |4 aut | |
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