Unusual mtDNA Control Region Length Heteroplasmy in the COS-7 Cell Line
The COS-7 cell line is a workhorse of virology research. To expand this cell line's utility and to enable studies on mitochondrial DNA (mtDNA) transcription and replication, we determined the complete nucleotide sequence of its mitochondrial genome by Sanger sequencing. In contrast to other available mtDNA sequences from Chlorocebus aethiops, the mtDNA of the COS-7 cell line was found to contain a variable number of perfect copies of a 108 bp unit tandemly repeated in the control region. We established that COS-7 cells are heteroplasmic with at least two variants being present: with four and five repeat units. The analysis of the mitochondrial genome sequences from other primates revealed that tandem repeats are absent from examined mtDNA control regions of humans and great apes, but appear in lower primates, where they are present in a homoplasmic state. To our knowledge, this is the first report of mtDNA length heteroplasmy in primates.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:11 |
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| Enthalten in: |
Genes - 11(2020), 6 vom: 30. Mai |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Kozhukhar, Nataliya [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 19.03.2021 Date Revised 19.03.2021 published: Electronic Citation Status MEDLINE |
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| doi: |
10.3390/genes11060607 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM310664500 |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a The COS-7 cell line is a workhorse of virology research. To expand this cell line's utility and to enable studies on mitochondrial DNA (mtDNA) transcription and replication, we determined the complete nucleotide sequence of its mitochondrial genome by Sanger sequencing. In contrast to other available mtDNA sequences from Chlorocebus aethiops, the mtDNA of the COS-7 cell line was found to contain a variable number of perfect copies of a 108 bp unit tandemly repeated in the control region. We established that COS-7 cells are heteroplasmic with at least two variants being present: with four and five repeat units. The analysis of the mitochondrial genome sequences from other primates revealed that tandem repeats are absent from examined mtDNA control regions of humans and great apes, but appear in lower primates, where they are present in a homoplasmic state. To our knowledge, this is the first report of mtDNA length heteroplasmy in primates | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a Chlorocebus aethiops | |
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| 650 | 4 | |a mitochondrial DNA | |
| 650 | 4 | |a mtDNA length heteroplasmy | |
| 650 | 4 | |a variable number tandem repeats | |
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| 700 | 1 | |a Alexeyev, Mikhail F |e verfasserin |4 aut | |
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