Intelligent image-based deformation-assisted cell sorting with molecular specificity
Although label-free cell sorting is desirable for providing pristine cells for further analysis or use, current approaches lack molecular specificity and speed. Here, we combine real-time fluorescence and deformability cytometry with sorting based on standing surface acoustic waves and transfer molecular specificity to image-based sorting using an efficient deep neural network. In addition to general performance, we demonstrate the utility of this method by sorting neutrophils from whole blood without labels.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:17 |
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| Enthalten in: |
Nature methods - 17(2020), 6 vom: 25. Juni, Seite 595-599 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Nawaz, Ahmad Ahsan [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 10.09.2020 Date Revised 02.02.2021 published: Print-Electronic figshare: 10.6084/m9.figshare.11302595 Citation Status MEDLINE |
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| doi: |
10.1038/s41592-020-0831-y |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM310336260 |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Although label-free cell sorting is desirable for providing pristine cells for further analysis or use, current approaches lack molecular specificity and speed. Here, we combine real-time fluorescence and deformability cytometry with sorting based on standing surface acoustic waves and transfer molecular specificity to image-based sorting using an efficient deep neural network. In addition to general performance, we demonstrate the utility of this method by sorting neutrophils from whole blood without labels | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 700 | 1 | |a Urbanska, Marta |e verfasserin |4 aut | |
| 700 | 1 | |a Herbig, Maik |e verfasserin |4 aut | |
| 700 | 1 | |a Nötzel, Martin |e verfasserin |4 aut | |
| 700 | 1 | |a Kräter, Martin |e verfasserin |4 aut | |
| 700 | 1 | |a Rosendahl, Philipp |e verfasserin |4 aut | |
| 700 | 1 | |a Herold, Christoph |e verfasserin |4 aut | |
| 700 | 1 | |a Toepfner, Nicole |e verfasserin |4 aut | |
| 700 | 1 | |a Kubánková, Markéta |e verfasserin |4 aut | |
| 700 | 1 | |a Goswami, Ruchi |e verfasserin |4 aut | |
| 700 | 1 | |a Abuhattum, Shada |e verfasserin |4 aut | |
| 700 | 1 | |a Reichel, Felix |e verfasserin |4 aut | |
| 700 | 1 | |a Müller, Paul |e verfasserin |4 aut | |
| 700 | 1 | |a Taubenberger, Anna |e verfasserin |4 aut | |
| 700 | 1 | |a Girardo, Salvatore |e verfasserin |4 aut | |
| 700 | 1 | |a Jacobi, Angela |e verfasserin |4 aut | |
| 700 | 1 | |a Guck, Jochen |e verfasserin |4 aut | |
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