Alteration in faecal bile acids, gut microbial composition and diversity after laparoscopic sleeve gastrectomy
© 2020 BJS Society Ltd Published by John Wiley & Sons Ltd..
BACKGROUND: Laparoscopic sleeve gastrectomy (LSG) is a well established treatment for severe obesity and type 2 diabetes. Although the gut microbiota is linked to the efficacy of LSG, the underlying mechanisms remain elusive. The effect of LSG for morbid obesity on the gut microbiota and bile acids was assessed here.
METHODS: Severely obese subjects who were candidates for LSG were included and followed until 6 months after surgery. The composition and abundance of the microbiota and bile acids in faeces were assessed by 16S ribosomal RNA sequencing, quantitative PCR and liquid chromatography-mass spectrometry.
RESULTS: In total, 28 patients with a mean(s.d.) BMI of 44·2(6·6) kg/m2 were enrolled. These patients had achieved excess weight loss of 53·2(19·0) per cent and showed improvement in metabolic diseases by 6 months after LSG, accompanied by an alteration in the faecal microbial community. The increase in α-diversity and abundance of specific taxa, such as Rikenellaceae and Christensenellaceae, was strongly associated with reduced faecal bile acid levels. These changes had a significant positive association with excess weight loss and metabolic alterations. However, the total number of faecal bacteria was lower in patients before (mean(s.d.) 10·26(0·36) log10 cells per g faeces) and after (10·39(0·29) log10 cells per g faeces) operation than in healthy subjects (10·83(0·27) log10 cells per g faeces).
CONCLUSION: LSG is associated with a reduction in faecal bile acids and greater abundance of specific bacterial taxa and α-diversity that may contribute to the metabolic changes.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:107 |
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| Enthalten in: |
The British journal of surgery - 107(2020), 12 vom: 20. Nov., Seite 1673-1685 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Ikeda, T [VerfasserIn] |
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| Links: |
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| Themen: |
Bile Acids and Salts |
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| Anmerkungen: |
Date Completed 02.03.2021 Date Revised 02.03.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1002/bjs.11654 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM310147557 |
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| 245 | 1 | 0 | |a Alteration in faecal bile acids, gut microbial composition and diversity after laparoscopic sleeve gastrectomy |
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| 500 | |a Date Revised 02.03.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2020 BJS Society Ltd Published by John Wiley & Sons Ltd. | ||
| 520 | |a BACKGROUND: Laparoscopic sleeve gastrectomy (LSG) is a well established treatment for severe obesity and type 2 diabetes. Although the gut microbiota is linked to the efficacy of LSG, the underlying mechanisms remain elusive. The effect of LSG for morbid obesity on the gut microbiota and bile acids was assessed here | ||
| 520 | |a METHODS: Severely obese subjects who were candidates for LSG were included and followed until 6 months after surgery. The composition and abundance of the microbiota and bile acids in faeces were assessed by 16S ribosomal RNA sequencing, quantitative PCR and liquid chromatography-mass spectrometry | ||
| 520 | |a RESULTS: In total, 28 patients with a mean(s.d.) BMI of 44·2(6·6) kg/m2 were enrolled. These patients had achieved excess weight loss of 53·2(19·0) per cent and showed improvement in metabolic diseases by 6 months after LSG, accompanied by an alteration in the faecal microbial community. The increase in α-diversity and abundance of specific taxa, such as Rikenellaceae and Christensenellaceae, was strongly associated with reduced faecal bile acid levels. These changes had a significant positive association with excess weight loss and metabolic alterations. However, the total number of faecal bacteria was lower in patients before (mean(s.d.) 10·26(0·36) log10 cells per g faeces) and after (10·39(0·29) log10 cells per g faeces) operation than in healthy subjects (10·83(0·27) log10 cells per g faeces) | ||
| 520 | |a CONCLUSION: LSG is associated with a reduction in faecal bile acids and greater abundance of specific bacterial taxa and α-diversity that may contribute to the metabolic changes | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
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| 650 | 7 | |a RNA, Ribosomal, 16S |2 NLM | |
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| 700 | 1 | |a Yoshida, Y |e verfasserin |4 aut | |
| 700 | 1 | |a Matsumoto, S |e verfasserin |4 aut | |
| 700 | 1 | |a Tanaka, M |e verfasserin |4 aut | |
| 700 | 1 | |a Nakayama, J |e verfasserin |4 aut | |
| 700 | 1 | |a Nagao, Y |e verfasserin |4 aut | |
| 700 | 1 | |a Nakata, R |e verfasserin |4 aut | |
| 700 | 1 | |a Oki, E |e verfasserin |4 aut | |
| 700 | 1 | |a Akahoshi, T |e verfasserin |4 aut | |
| 700 | 1 | |a Okano, S |e verfasserin |4 aut | |
| 700 | 1 | |a Nomura, M |e verfasserin |4 aut | |
| 700 | 1 | |a Hashizume, M |e verfasserin |4 aut | |
| 700 | 1 | |a Maehara, Y |e verfasserin |4 aut | |
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