Heterogeneous T cell motility behaviors emerge from a coupling between speed and turning in vivo
© 2020, Jerison and Quake..
T cells in vivo migrate primarily via undirected random walks, but it remains unresolved how these random walks generate an efficient search. Here, we use light sheet microscopy of T cells in the larval zebrafish as a model system to study motility across large populations of cells over hours in their native context. We show that cells do not perform Levy flight; rather, there is substantial cell-to-cell variability in speed, which persists over timespans of a few hours. This variability is amplified by a correlation between speed and directional persistence, generating a characteristic cell behavioral manifold that is preserved under a perturbation to cell speeds, and seen in Mouse T cells and Dictyostelium. Together, these effects generate a broad range of length scales over which cells explore in vivo.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:9 |
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| Enthalten in: |
eLife - 9(2020) vom: 19. Mai |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Jerison, Elizabeth R [VerfasserIn] |
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| Links: |
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| Themen: |
Cell motility |
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| Anmerkungen: |
Date Completed 23.03.2021 Date Revised 23.03.2021 published: Electronic GEO: GSE137770 Citation Status MEDLINE |
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| doi: |
10.7554/eLife.53933 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a © 2020, Jerison and Quake. | ||
| 520 | |a T cells in vivo migrate primarily via undirected random walks, but it remains unresolved how these random walks generate an efficient search. Here, we use light sheet microscopy of T cells in the larval zebrafish as a model system to study motility across large populations of cells over hours in their native context. We show that cells do not perform Levy flight; rather, there is substantial cell-to-cell variability in speed, which persists over timespans of a few hours. This variability is amplified by a correlation between speed and directional persistence, generating a characteristic cell behavioral manifold that is preserved under a perturbation to cell speeds, and seen in Mouse T cells and Dictyostelium. Together, these effects generate a broad range of length scales over which cells explore in vivo | ||
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