Novel Medicinal Mushroom Blend as a Promising Supplement in Integrative Oncology : A Multi-Tiered Study using 4T1 Triple-Negative Mouse Breast Cancer Model
Although medicinal mushroom extracts have been proposed as promising anti-cancer agents, their precise impacts on metastatic breast cancer are still to be clarified. For this purpose, the present study exploited the effect of a novel medicinal mushroom blend, namely Micotherapy U-care, in a 4T1 triple-negative mouse breast cancer model. Mice were orally administered with Micotherapy U-care, consisting of a mixture of Agaricus blazei, Ophiocordyceps sinensis, Ganoderma lucidum, Grifola frondosa, and Lentinula edodes. The syngeneic tumor-bearing mice were generated by injecting 4T1 cells in both supplemented and non-supplemented mice. After sacrifice 25 days later, specific endpoints and pathological outcomes of the murine pulmonary tissue were evaluated. (i) Histopathological and ultrastructural analysis and (ii) immunohistochemical assessment of TGF-ß1, IL-6 and NOS2, COX2, SOD1 as markers of inflammation and oxidative stress were performed. The QoL was comparatively evaluated. Micotherapy U-care supplementation, starting before 4T1 injection and lasting until the end of the experiment, dramatically reduced the pulmonary metastases density, also triggering a decrease of fibrotic response, and reducing IL-6, NOS, and COX2 expression. SOD1 and TGF-ß1 results were also discussed. These findings support the valuable potential of Micotherapy U-care as adjuvant therapy in the critical management of triple-negative breast cancer.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:21 |
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| Enthalten in: |
International journal of molecular sciences - 21(2020), 10 vom: 14. Mai |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Roda, Elisa [VerfasserIn] |
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| Links: |
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| Themen: |
Agaricus blazei |
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| Anmerkungen: |
Date Completed 11.02.2021 Date Revised 11.02.2021 published: Electronic Citation Status MEDLINE |
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| doi: |
10.3390/ijms21103479 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM310057345 |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Although medicinal mushroom extracts have been proposed as promising anti-cancer agents, their precise impacts on metastatic breast cancer are still to be clarified. For this purpose, the present study exploited the effect of a novel medicinal mushroom blend, namely Micotherapy U-care, in a 4T1 triple-negative mouse breast cancer model. Mice were orally administered with Micotherapy U-care, consisting of a mixture of Agaricus blazei, Ophiocordyceps sinensis, Ganoderma lucidum, Grifola frondosa, and Lentinula edodes. The syngeneic tumor-bearing mice were generated by injecting 4T1 cells in both supplemented and non-supplemented mice. After sacrifice 25 days later, specific endpoints and pathological outcomes of the murine pulmonary tissue were evaluated. (i) Histopathological and ultrastructural analysis and (ii) immunohistochemical assessment of TGF-ß1, IL-6 and NOS2, COX2, SOD1 as markers of inflammation and oxidative stress were performed. The QoL was comparatively evaluated. Micotherapy U-care supplementation, starting before 4T1 injection and lasting until the end of the experiment, dramatically reduced the pulmonary metastases density, also triggering a decrease of fibrotic response, and reducing IL-6, NOS, and COX2 expression. SOD1 and TGF-ß1 results were also discussed. These findings support the valuable potential of Micotherapy U-care as adjuvant therapy in the critical management of triple-negative breast cancer | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Agaricus blazei | |
| 650 | 4 | |a Ganoderma lucidum | |
| 650 | 4 | |a Grifola frondosa | |
| 650 | 4 | |a Lentinula edodes | |
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| 650 | 4 | |a breast cancer | |
| 650 | 4 | |a complementary medicine | |
| 650 | 4 | |a in vivo | |
| 650 | 4 | |a lung metastasis | |
| 650 | 4 | |a mycotherapy | |
| 700 | 1 | |a Luca, Fabrizio De |e verfasserin |4 aut | |
| 700 | 1 | |a Iorio, Carmine Di |e verfasserin |4 aut | |
| 700 | 1 | |a Ratto, Daniela |e verfasserin |4 aut | |
| 700 | 1 | |a Siciliani, Stella |e verfasserin |4 aut | |
| 700 | 1 | |a Ferrari, Beatrice |e verfasserin |4 aut | |
| 700 | 1 | |a Cobelli, Filippo |e verfasserin |4 aut | |
| 700 | 1 | |a Borsci, Giuseppina |e verfasserin |4 aut | |
| 700 | 1 | |a Priori, Erica Cecilia |e verfasserin |4 aut | |
| 700 | 1 | |a Chinosi, Silvia |e verfasserin |4 aut | |
| 700 | 1 | |a Ronchi, Andrea |e verfasserin |4 aut | |
| 700 | 1 | |a Franco, Renato |e verfasserin |4 aut | |
| 700 | 1 | |a Di Francia, Raffaele |e verfasserin |4 aut | |
| 700 | 1 | |a Berretta, Massimiliano |e verfasserin |4 aut | |
| 700 | 1 | |a Locatelli, Carlo Alessandro |e verfasserin |4 aut | |
| 700 | 1 | |a Gregori, Andrej |e verfasserin |4 aut | |
| 700 | 1 | |a Savino, Elena |e verfasserin |4 aut | |
| 700 | 1 | |a Bottone, Maria Grazia |e verfasserin |4 aut | |
| 700 | 1 | |a Rossi, Paola |e verfasserin |4 aut | |
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